A novel nano-copper-bearing stainless steel with reduced Cu2+ release only inducing transient foreign body reaction via affecting the activity of NF-kappa B and Caspase 3

doi:10.2147/IJN.S90249

IMR OpenIR

	A novel nano-copper-bearing stainless steel with reduced Cu2+ release only inducing transient foreign body reaction via affecting the activity of NF-kappa B and Caspase 3
	Wang, Lei 1; Ren, Ling 2; Tang, Tingting 1; Dai, Kerong 1; Yang, Ke 2; Hao, Yongqiang 1
通讯作者	Hao, Yongqiang(hyq_9hospital@hotmail.com)
	2015
发表期刊	INTERNATIONAL JOURNAL OF NANOMEDICINE
ISSN	1178-2013
卷号	10 页码:6725-6739
摘要	Foreign body reaction induced by biomaterials is a serious problem in clinical applications. Although 317L-Cu stainless steel (317L-Cu SS) is a new type of implant material with antibacterial ability and osteogenic property, the foreign body reaction level still needs to be assessed due to its Cu2+ releasing property. For this purpose, two macrophage cell lines were selected to detect cellular proliferation, apoptosis, mobility, and the secretions of inflammatory cytokines with the influence of 317L-Cu SS. Our results indicated that 317L-Cu SS had no obvious effect on the proliferation and apoptosis of macrophages; however, it significantly increased cellular migration and TNF-alpha secretion. Then, C57 mice were used to assess foreign body reaction induced by 317L-Cu SS. We observed significantly enhanced recruitment of inflammatory cells (primarily macrophages) with increased TNF-alpha secretion and apoptosis level in tissues around the materials in the early stage of implantation. With tissue healing, both inflammation and apoptosis significantly decreased. Further, we discovered that NF-kappa B pathway and Caspase 3 played important roles in 317L-Cu SS induced inflammation and apoptosis. We concluded that 317L-Cu SS could briefly promote the inflammation and apoptosis of surrounding tissues by regulating the activity of NF-kappa B pathway and Caspase 3. All these discoveries demonstrated that 317L-Cu SS has a great potential for clinical application.
关键词	nano-copper foreign body reaction inflammation apoptosis NF-kappa B Caspase 3
资助者	Key National Basic Research Program of China ; National Natural Science Foundation of China ; Industry-University-Research-Medical Cooperation Project of the Science and Technology Commission of Shanghai ; New Cutting-Edge Technology Project of ShenKang Hospital Development Center of Shanghai ; Doctoral Innovation Fund Projects from Shanghai Jiao Tong University School of Medicine
DOI	10.2147/IJN.S90249
收录类别	SCI
语种	英语
资助项目	Key National Basic Research Program of China[2012CB619101] ; National Natural Science Foundation of China[81071472] ; National Natural Science Foundation of China[81371960] ; National Natural Science Foundation of China[81301329] ; Industry-University-Research-Medical Cooperation Project of the Science and Technology Commission of Shanghai[12DZ1940203] ; New Cutting-Edge Technology Project of ShenKang Hospital Development Center of Shanghai[SHDC12014124] ; Doctoral Innovation Fund Projects from Shanghai Jiao Tong University School of Medicine[BXJ201429]
WOS研究方向	Science & Technology - Other Topics ; Pharmacology & Pharmacy
WOS类目	Nanoscience & Nanotechnology ; Pharmacology & Pharmacy
WOS记录号	WOS:000363780300001
出版者	DOVE MEDICAL PRESS LTD
引用统计	被引频次：21[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/121020
专题	中国科学院金属研究所
通讯作者	Hao, Yongqiang
作者单位	1.Shanghai Jiao Tong Univ, Sch Med, Shanghai Peoples Hosp 9, Shanghai Key Lab Orthopaed Implants,Dept Orthopae, Shanghai 200011, Peoples R China 2.Chinese Acad Sci, Met Res Inst, Shenyang 110016, Peoples R China
推荐引用方式 GB/T 7714	Wang, Lei,Ren, Ling,Tang, Tingting,et al. A novel nano-copper-bearing stainless steel with reduced Cu2+ release only inducing transient foreign body reaction via affecting the activity of NF-kappa B and Caspase 3[J]. INTERNATIONAL JOURNAL OF NANOMEDICINE,2015,10:6725-6739.
APA	Wang, Lei,Ren, Ling,Tang, Tingting,Dai, Kerong,Yang, Ke,&Hao, Yongqiang.(2015).A novel nano-copper-bearing stainless steel with reduced Cu2+ release only inducing transient foreign body reaction via affecting the activity of NF-kappa B and Caspase 3.INTERNATIONAL JOURNAL OF NANOMEDICINE,10,6725-6739.
MLA	Wang, Lei,et al."A novel nano-copper-bearing stainless steel with reduced Cu2+ release only inducing transient foreign body reaction via affecting the activity of NF-kappa B and Caspase 3".INTERNATIONAL JOURNAL OF NANOMEDICINE 10(2015):6725-6739.