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Bioinformatic and Expression Analyses of Genes Mediating Zinc Homeostasis in Nostoc punctiforme
Hudek, Lee1; Rai, L. C.2; Freestone, David1; Michalczyk, Agnes1; Gibson, Maria1; Song, Y. F.3; Ackland, M. Leigh1
Corresponding AuthorAckland, M. Leigh(leigha@deakin.edu.au)
2009-02-01
Source PublicationAPPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN0099-2240
Volume75Issue:3Pages:784-791
AbstractZinc homeostasis was investigated in Nostoc punctiforme. Cell tolerance to Zn(2+) over 14 days showed that ZnCl(2) levels above 22 mu M significantly reduced cell viability. After 3 days in 22 mu M ZnCl(2), ca. 12% of the Zn(2+) was in an EDTA-resistant component, suggesting an intracellular localization. Zinquin fluorescence was detected within cells exposed to concentrations up to 37 mu M relative to 0 mu M treatment. Radiolabeled (65)Zn showed Zn(2+) uptake increased over a 3-day period, while efflux occurred more rapidly within a 3-h time period. Four putative genes involved in Zn(2+) uptake and efflux in N. punctiforme were identified: (i) the predicted Co/Zn/Cd cation transporter, putative CDF; (ii) the predicted divalent heavy-metal cation transporter, putative Zip; (iii) the ATPase component and Fe/Zn uptake regulation protein, putative Fur; and (iv) an ABC-type Mn/Zn transport system, putative zinc ZnuC, ZnuABC system component. Quantitative real-time PCR indicated the responsiveness of all four genes to 22 mu M ZnCl(2) within 3 h, followed by a reduction to below basal levels after 24 h by putative ZIP, ZnuC, and Fur and a reduction to below basal level after 72 h by putative CDF efflux gene. These results demonstrate differential regulation of zinc transporters over time, indicating a role for them in zinc homeostasis in N. punctiforme.
Funding OrganizationInternational Science Linkage Programme ; Australia-China Special Fund for Scientific and Technological Cooperation.
DOI10.1128/AEM.02481-08
Indexed BySCI
Language英语
Funding ProjectInternational Science Linkage Programme ; Australia-China Special Fund for Scientific and Technological Cooperation.
WOS Research AreaBiotechnology & Applied Microbiology ; Microbiology
WOS SubjectBiotechnology & Applied Microbiology ; Microbiology
WOS IDWOS:000262690100029
PublisherAMER SOC MICROBIOLOGY
Citation statistics
Cited Times:17[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/126314
Collection中国科学院金属研究所
Corresponding AuthorAckland, M. Leigh
Affiliation1.Deakin Univ, Ctr Cellular & Mol Biol, Sch Life & Environm Sci, Burwood, Vic 3125, Australia
2.Banaras Hindu Univ, CAS Bot, Algal Biol Lab, Mol Biol Unit, Varanasi 221005, Uttar Pradesh, India
3.Chinese Acad Sci, Inst Appl Ecol, Key Lab Terr Ecol Proc, Shenyang, Peoples R China
Recommended Citation
GB/T 7714
Hudek, Lee,Rai, L. C.,Freestone, David,et al. Bioinformatic and Expression Analyses of Genes Mediating Zinc Homeostasis in Nostoc punctiforme[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2009,75(3):784-791.
APA Hudek, Lee.,Rai, L. C..,Freestone, David.,Michalczyk, Agnes.,Gibson, Maria.,...&Ackland, M. Leigh.(2009).Bioinformatic and Expression Analyses of Genes Mediating Zinc Homeostasis in Nostoc punctiforme.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,75(3),784-791.
MLA Hudek, Lee,et al."Bioinformatic and Expression Analyses of Genes Mediating Zinc Homeostasis in Nostoc punctiforme".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 75.3(2009):784-791.
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