IMR OpenIR
Nano-copper-bearing stainless steel promotes fracture healing by accelerating the callus evolution process
Wang, Lei1; Li, Guoyuan1; Ren, Ling2; Kong, Xiangdong1; Wang, Yugang1; Han, Xiuguo1; Jiang, Wenbo3; Dai, Kerong1; Yang, Ke2; Hao, Yongqiang1
Corresponding AuthorYang, Ke(kyang@imr.ac.cn) ; Hao, Yongqiang(hyq_9hospital@hotmail.com)
2017
Source PublicationINTERNATIONAL JOURNAL OF NANOMEDICINE
ISSN1178-2013
Volume12Pages:8443-8457
AbstractTreatment for fractures requires internal fixation devices, which are mainly produced from stainless steel or titanium alloy without biological functions. Therefore, we developed a novel nano-copper-bearing stainless steel with nano-sized copper-precipitation (317L-Cu SS). Based on previous studies, this work explores the effect of 317L-Cu SS on fracture healing; that is, proliferation, osteogenic differentiation, osteogenesis-related gene expression, and lysyl oxidase activity of human bone mesenchymal stem cells were detected in vitro. Sprague Dawley rats were used to build an animal fracture model, and fracture healing and callus evolution were investigated by radiology (X-ray and micro-CT), histology (II&E, Masson, and safranin O/fast green staining), and histomorphometry. Further, the Cu2+ content and It unx2 level in the callus were determined, and local mechanical test of the fracture was performed to assess the healing quality. Our results revealed that 317L-Cu SS did not affect the proliferation of human bone mesenchymal stem cells, but promoted osteogenic differentiation and the expression of osteogenesis-related genes. In addition, 317L-Cu SS upregulated the lysyl oxidase activity. The X-ray and micro-CT results showed that the callus evolution efficiency and fracture healing speed were superior for 317L-Cu SS. Histological staining displayed large amounts of fibrous tissues at 3 weeks, and cartilage and new bone at 6 weeks. Further, histomorphometric analysis indicated that the callus possessed higher osteogenic efficiency at 6 weeks, and a high Cu2+ content and increased Runx2 expression were observed in the callus for 317L-Cu SS. Besides, the mechanical strength of the fracture site Was much hotter than that of the control group. Overall, we conclude that 317L-Cu SS possesses the ability to increase Cu2+ content and promote osteogenesis in the callus, which could accelerate the callus evolution process and bone formation to provide faster and better fracture healing.
Keywordnano-sized copper lysyl oxidise osteogenesis fracture healing callus evolution
Funding OrganizationNational Key Research and Development Program of China ; National Natural Science Foundation of China ; Key National Basic Research Program of China ; Technology Support Project of the Science and Technology Commission of Shanghai ; New Cutting-Edge Technology Project of ShenKang Hospital Development Center of Shanghai ; Shanghai Jiao Tong University Cross Research Fund of Medical Engineering ; Multicenter Clinical Research Project of Shanghai Jiao Tong University School of Medicine ; Doctoral Innovation Fund Projects from Shanghai Jiao Tong University School of Medicine
DOI10.2147/IJN.SI46866
Indexed BySCI
Language英语
Funding ProjectNational Key Research and Development Program of China[2016YFC1100600] ; National Natural Science Foundation of China[81071472] ; National Natural Science Foundation of China[81371960] ; National Natural Science Foundation of China[51631009] ; Key National Basic Research Program of China[2012CB619101] ; Technology Support Project of the Science and Technology Commission of Shanghai[13441901302] ; Technology Support Project of the Science and Technology Commission of Shanghai[14441901000] ; Technology Support Project of the Science and Technology Commission of Shanghai[15411951200] ; New Cutting-Edge Technology Project of ShenKang Hospital Development Center of Shanghai[SHDC12014124] ; Shanghai Jiao Tong University Cross Research Fund of Medical Engineering[YG2013MS57] ; Multicenter Clinical Research Project of Shanghai Jiao Tong University School of Medicine[DLY201506] ; Doctoral Innovation Fund Projects from Shanghai Jiao Tong University School of Medicine[BXJ201429]
WOS Research AreaScience & Technology - Other Topics ; Pharmacology & Pharmacy
WOS SubjectNanoscience & Nanotechnology ; Pharmacology & Pharmacy
WOS IDWOS:000416144200001
PublisherDOVE MEDICAL PRESS LTD
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/127096
Collection中国科学院金属研究所
Corresponding AuthorYang, Ke; Hao, Yongqiang
Affiliation1.Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Dept Orthopaed, Shanghai Key Lab Orthopaed Implants,Sch Med, 639 Zhizaoju Rd, Shanghai 200011, Peoples R China
2.Chinese Acad Sci, Inst Met Res, Special Mat & Device Res Dept, Shenyang, Peoples R China
3.Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Sch Med, Med Printing Innovat Res Ctr 3D, Shanghai, Peoples R China
Recommended Citation
GB/T 7714
Wang, Lei,Li, Guoyuan,Ren, Ling,et al. Nano-copper-bearing stainless steel promotes fracture healing by accelerating the callus evolution process[J]. INTERNATIONAL JOURNAL OF NANOMEDICINE,2017,12:8443-8457.
APA Wang, Lei.,Li, Guoyuan.,Ren, Ling.,Kong, Xiangdong.,Wang, Yugang.,...&Hao, Yongqiang.(2017).Nano-copper-bearing stainless steel promotes fracture healing by accelerating the callus evolution process.INTERNATIONAL JOURNAL OF NANOMEDICINE,12,8443-8457.
MLA Wang, Lei,et al."Nano-copper-bearing stainless steel promotes fracture healing by accelerating the callus evolution process".INTERNATIONAL JOURNAL OF NANOMEDICINE 12(2017):8443-8457.
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