Dynamic Model for Characterizing Contractile Behaviors and Mechanical Properties of a Cardiomyocyte

doi:10.1016/j.bpj.2017.11.002

IMR OpenIR

	Dynamic Model for Characterizing Contractile Behaviors and Mechanical Properties of a Cardiomyocyte
	Zhang, Chuang 1,2; Wang, Wenxue 1; He, Wenhui 3; Xi, Ning 4; Wang, Yuechao 1; Liu, Lianqing 1
通讯作者	Wang, Wenxue(wangwenxue@sia.cn) ; Liu, Lianqing(lqliu@sia.cn)
	2018-01-09
发表期刊	BIOPHYSICAL JOURNAL
ISSN	0006-3495
卷号	114 期号:1 页码:188-200
摘要	Studies on the contractile dynamics of heart cells have attracted broad attention for the development of both heart disease therapies and cardiomyocyte-actuated micro-robotics. In this study, a linear dynamic model of a single cardiomyocyte cell was proposed at the subcellular scale to characterize the contractile behaviors of heart cells, with system parameters representing the mechanical properties of the subcellular components of living cardiomyocytes. The system parameters of the dynamic model were identified with the cellular beating pattern measured by a scanning ion conductance microscope. The experiments were implemented with cardiomyocytes in one control group and two experimental groups with the drugs cytochalasin-D or nocodazole, to identify the system parameters of the model based on scanning ion conductance microscope measurements, measurement of the cellular Young's modulus with atomic force microscopy indentation, measurement of cellular contraction forces using the micro-pillar technique, and immunofluorescence staining and imaging of the cytoskeleton. The proposed mathematical model was both indirectly and qualitatively verified by the variation in cytoskeleton, beating amplitude, and contractility of cardiomyocytes among the control and the experimental groups, as well as directly and quantitatively validated by the simulation and the significant consistency of 90.5% in the comparison between the ratios of the Young's modulus and the equivalent comprehensive cellular elasticities of cells in the experimental groups to those in the control group. Apart from mechanical properties (mass, elasticity, and viscosity) of subcellular structures, other properties of cardiomyocytes have also been studied, such as the properties of the relative action potential pattern and cellular beating frequency. This work has potential implications for research on cytobiology, drug screening, mechanisms of the heart, and cardiomyocyte-based bio-syncretic robotics.
资助者	National Natural Science Foundation of China ; Chinese Academy of Sciences/State Administration of Foreign Experts Affairs (CAS/SAFEA) International Partnership Program for Creative Research Teams
DOI	10.1016/j.bpj.2017.11.002
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[61673372] ; National Natural Science Foundation of China[61433017] ; National Natural Science Foundation of China[61327014] ; National Natural Science Foundation of China[61522312] ; Chinese Academy of Sciences/State Administration of Foreign Experts Affairs (CAS/SAFEA) International Partnership Program for Creative Research Teams
WOS研究方向	Biophysics
WOS类目	Biophysics
WOS记录号	WOS:000419595500020
出版者	CELL PRESS
引用统计	被引频次：22[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/127250
专题	中国科学院金属研究所
通讯作者	Wang, Wenxue; Liu, Lianqing
作者单位	1.Chinese Acad Sci, Shenyang Inst Automat, State Key Lab Robot, Shenyang, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Shenyang Pharmaceut Univ, Dept Pharmacol, Shenyang, Peoples R China 4.Univ Hong Kong Pokfulam, Emerging Technol Inst, Dept Ind & Manufacturing Syst Engn, Hong Kong, Hong Kong, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Chuang,Wang, Wenxue,He, Wenhui,et al. Dynamic Model for Characterizing Contractile Behaviors and Mechanical Properties of a Cardiomyocyte[J]. BIOPHYSICAL JOURNAL,2018,114(1):188-200.
APA	Zhang, Chuang,Wang, Wenxue,He, Wenhui,Xi, Ning,Wang, Yuechao,&Liu, Lianqing.(2018).Dynamic Model for Characterizing Contractile Behaviors and Mechanical Properties of a Cardiomyocyte.BIOPHYSICAL JOURNAL,114(1),188-200.
MLA	Zhang, Chuang,et al."Dynamic Model for Characterizing Contractile Behaviors and Mechanical Properties of a Cardiomyocyte".BIOPHYSICAL JOURNAL 114.1(2018):188-200.