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Anti-fibrotic function of Cu-bearing stainless steel for reducing recurrence of urethral stricture after stent implantation
Zhao, Jing; Ren, Ling; Liu, Meixia; Xi, Tong; Zhang, Bingchun; Yang, Ke
Corresponding AuthorRen, Ling(lren@imr.ac.cn) ; Yang, Ke(kyang@imr.ac.cn)
2018-07-01
Source PublicationJOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
ISSN1552-4973
Volume106Issue:5Pages:2019-2028
AbstractRecurrent stenosis is the main reason inducing the failure of urethral stricture treatment. Our previous study has found that the 316L type Cu bearing stainless steel (316L-Cu SS) showed antimicrobial activity and anti-encrustation performance when it was used for relieving urethral obstructer. However, whether it can reduce the occurrence of fibrosis or not, we need further investigation to compare the cellular and molecular responses of human urethral scar fibroblast cells (USFCs) on 316L-Cu SS and medical grade 316L stainless (316L SS, as a control). [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4- sulfophenyl)- 2H-tetrazolium (MTS) and Transwell were used to assess the cellular responses, which confirmed that 316L-Cu SS could inhibit proliferation and migration of USFCs. Molecular expressions of fibrosis were evaluated by western blot, real-time quantitative polymerase chain reaction (qPCR), and Cu/Zn superoxide dismutase (CuZnSOD) measurement. The results indicated that up-regulating of CuZnSOD attenuated the transforming growth factor-1 expression and phosphorylation of Smad3 after exposure to 316L-Cu SS. Besides, the content of collagen type I (COL1) and collagen type III (COL3) secreting into the culture medium measured by enzyme-linked immunosorbent assay were in accord with the results of messenger ribonucleic acids. Both of them exhibited lower levels of COL1/COL3 exposure to 316L-Cu SS, demonstrating the inhibitory performance of 316L-Cu SS against fibrosis. (c) 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2019-2028, 2018.
KeywordCu bearing stainless steel fibrosis CuZnSOD TGF-beta 1 Smad3 COL1 COL3
Funding OrganizationNational Basic Research Program of China ; National Natural Science Foundation of China ; Foundation of Jiangsu Collaborative Innovation Center of Biomedical Functional Materials
DOI10.1002/jbm.b.34005
Indexed BySCI
Language英语
Funding ProjectNational Basic Research Program of China[2012CB619101] ; National Natural Science Foundation of China[51171186] ; Foundation of Jiangsu Collaborative Innovation Center of Biomedical Functional Materials
WOS Research AreaEngineering ; Materials Science
WOS SubjectEngineering, Biomedical ; Materials Science, Biomaterials
WOS IDWOS:000435443500038
PublisherWILEY
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/128637
Collection中国科学院金属研究所
Corresponding AuthorRen, Ling; Yang, Ke
AffiliationChinese Acad Sci, Inst Met Res, Shenyang 110016, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Jing,Ren, Ling,Liu, Meixia,et al. Anti-fibrotic function of Cu-bearing stainless steel for reducing recurrence of urethral stricture after stent implantation[J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS,2018,106(5):2019-2028.
APA Zhao, Jing,Ren, Ling,Liu, Meixia,Xi, Tong,Zhang, Bingchun,&Yang, Ke.(2018).Anti-fibrotic function of Cu-bearing stainless steel for reducing recurrence of urethral stricture after stent implantation.JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS,106(5),2019-2028.
MLA Zhao, Jing,et al."Anti-fibrotic function of Cu-bearing stainless steel for reducing recurrence of urethral stricture after stent implantation".JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS 106.5(2018):2019-2028.
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