Microbial Community Structure and Functional Potential in Cultivated and Native Tallgrass Prairie Soils of the Midwestern United States

doi:10.3389/fmicb.2018.01775

IMR OpenIR

	Microbial Community Structure and Functional Potential in Cultivated and Native Tallgrass Prairie Soils of the Midwestern United States
	Mackelprang, Rachel 1; Grube, Alyssa M.2; Lamendella, Regina 2; Jesus, Ederson da C.3,4; Copeland, Alex 5; Liang, Chao 4,6; Jackson, Randall D.4,7; Rice, Charles W.8; Kapucija, Stefanie 1; Parsa, Bayan 1; Tringe, Susannah G.5; Tiedje, James M.3,4; Jansson, Janet K.9
通讯作者	Tringe, Susannah G.(sgtringe@lbl.gov) ; Tiedje, James M.(tiedjej@msu.edu) ; Jansson, Janet K.(janet.jansson@pnnl.gov)
	2018-08-15
发表期刊	FRONTIERS IN MICROBIOLOGY
ISSN	1664-302X
卷号	9 页码:15
摘要	The North American prairie covered about 3.6 million-km(2) of the continent prior to European contact. Only 1-2% of the original prairie remains, but the soils that developed under these prairies are some of the most productive and fertile in the world, containing over 35% of the soil carbon in the continental United States. Cultivation may alter microbial diversity and composition, influencing the metabolism of carbon, nitrogen, and other dements. Here, we explored the structure and functional potential of the soil microbiome in paired cultivated-corn (at the time of sampling) and never-cultivated native prairie soils across a three-states transect (Wisconsin, Iowa, and Kansas) using metagenomic and 16S rRNA gene sequencing and lipid analysis. At the Wisconsin site, we also sampled adjacent restored prairie and switchgrass plots. We found that agricultural practices drove differences in community composition and diversity across the transect. Microbial biomass in prairie samples was twice that of cultivated soils, but alpha diversity was higher with cultivation. Metagenome analyses revealed denitrification and starch degradation genes were abundant across all soils, as were core genes involved in response to osmotic stress, resource transport, and environmental sensing. Together, these data indicate that cultivation shifted the microbiome in consistent ways across different regions of the prairie, but also suggest that many functions are resilient to changes caused by land management practices - perhaps reflecting adaptations to conditions common to tallgrass prairie soils in the region (e.g., soil type, parent material, development under grasses, temperature and rainfall patterns, and annual freeze-thaw cycles). These findings are important for understanding the long-term consequences of land management practices to prairie soil microbial communities and their genetic potential to carry out key functions.
关键词	soil microbiome land management metagenomics native prairie climate change carbon cycle nitrogen cycle
资助者	Office of Science of the U.S. Department of Energy ; DOE Great Lakes Bioenergy Research Center (DOE Office of Science BER) ; Laboratory Directed Research and Development Program at PNNL ; U.S. Department of Energy ; Howard Hughes Medical Institute through the Precollege and Undergraduate Science Education Program ; National Science Foundation ; NSF ; Research Coordination Network grant
DOI	10.3389/fmicb.2018.01775
收录类别	SCI
语种	英语
资助项目	Office of Science of the U.S. Department of Energy[DE-AC02-05CH11231] ; DOE Great Lakes Bioenergy Research Center (DOE Office of Science BER)[DE-FC02-07ER64494] ; Laboratory Directed Research and Development Program at PNNL ; U.S. Department of Energy[DE-AC05-76RL01830] ; Howard Hughes Medical Institute through the Precollege and Undergraduate Science Education Program ; National Science Foundation ; NSF[DBI-1248096] ; Research Coordination Network grant[RCN 1051481]
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:000441566600001
出版者	FRONTIERS MEDIA SA
引用统计	被引频次：39[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/128745
专题	中国科学院金属研究所
通讯作者	Tringe, Susannah G.; Tiedje, James M.; Jansson, Janet K.
作者单位	1.Calif State Univ Northridge, Dept Biol, Northridge, CA 91330 USA 2.Juniata Coll, Dept Biol, Huntingdon, PA 16652 USA 3.Michigan State Univ, Ctr Microbial Ecol, E Lansing, MI 48824 USA 4.Univ Wisconsin, US DOE, Great Lakes Bioenergy Res Ctr, Madison, WI 53706 USA 5.US DOE, Joint Genome Inst, Walnut Creek, CA 94598 USA 6.Chinese Acad Sci, Inst Appl Ecol, Shenyang, Liaoning, Peoples R China 7.Univ Wisconsin, Dept Agron, 1575 Linden Dr, Madison, WI 53706 USA 8.Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA 9.Pacific Northwest Natl Lab, Earth & Biol Sci Directorate, Richland, WA 99352 USA
推荐引用方式 GB/T 7714	Mackelprang, Rachel,Grube, Alyssa M.,Lamendella, Regina,et al. Microbial Community Structure and Functional Potential in Cultivated and Native Tallgrass Prairie Soils of the Midwestern United States[J]. FRONTIERS IN MICROBIOLOGY,2018,9:15.
APA	Mackelprang, Rachel.,Grube, Alyssa M..,Lamendella, Regina.,Jesus, Ederson da C..,Copeland, Alex.,...&Jansson, Janet K..(2018).Microbial Community Structure and Functional Potential in Cultivated and Native Tallgrass Prairie Soils of the Midwestern United States.FRONTIERS IN MICROBIOLOGY,9,15.
MLA	Mackelprang, Rachel,et al."Microbial Community Structure and Functional Potential in Cultivated and Native Tallgrass Prairie Soils of the Midwestern United States".FRONTIERS IN MICROBIOLOGY 9(2018):15.