Improvement of Endothelial Cell Migration on Nickel-Free Stainless Steel by the Involvement of Epithelial-Mesenchymal Transition

doi:10.1166/jbt.2018.1877

IMR OpenIR

	Improvement of Endothelial Cell Migration on Nickel-Free Stainless Steel by the Involvement of Epithelial-Mesenchymal Transition
	Li, Liming 1; Liu, Xiaqing 1; Guo, Peng 1; Ren, Yibin 2; Yang, Ke 2
通讯作者	Li, Liming()
	2018-09-01
发表期刊	JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING
ISSN	2157-9083
卷号	8 期号:9 页码:1320-1326
摘要	To understand the impact of high-nitrogen nickel-free austenitic stainless steel (HNNFSS) on the migration and epithelial-mesenchymal transition (EMT) of human umbilical vein endothelial cells (HUVECs) and the involved mechanisms, we conducted a comparative study of cellular and molecular responses of HUVECs to HNNFSS and 317L (austenitic 317L stainless steel). Cell Counting Kit-8 assay was used to analyze cell viability. The migration capabilities of HUVECs were analyzed using the wound healing and Transwell assays. Fluorescence staining was performed to observe the cell cytoskeleton. Quantitative real-time polymerase chain reaction and Western blot were conducted to analyze the gene expression and protein levels associated with migration and EMT-related markers. Our results showed that HNNFSS promoted HUVEC migration and increased matrix metalloproteinase (MMP)-2 and MMP-9 expression and activities. In addition, HNNFSS treatment induced cytoskeletal remodeling and increased the expression of related genes. The treatment also stimulated EMT as characterized by the decrease in E-cadherin and the increase in vimentin. Our results suggest that compared with 317L, HNNFSS enhances the migration capabilities of HUVECs and promotes EMT. The findings of this study provide valuable information fot developing new biomedical materials for stent implantation.
关键词	In-Stent Restenosis Nickel-Free Stainless Steel Migration Epithelial-Mesenchymal Transition
资助者	Fundamental Research Funds for the Central Universities
DOI	10.1166/jbt.2018.1877
收录类别	SCI
语种	英语
资助项目	Fundamental Research Funds for the Central Universities[N152004001] ; Fundamental Research Funds for the Central Universities[N172004007]
WOS研究方向	Cell Biology
WOS类目	Cell & Tissue Engineering
WOS记录号	WOS:000448343200013
出版者	AMER SCIENTIFIC PUBLISHERS
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/130054
专题	中国科学院金属研究所
通讯作者	Li, Liming
作者单位	1.Northeastern Univ, Coll Life & Hlth Sci, Inst Biotechnol, Shenyang 110819, Liaoning, Peoples R China 2.Chinese Acad Sci, Inst Met Res, Shenyang 110016, Liaoning, Peoples R China
推荐引用方式 GB/T 7714	Li, Liming,Liu, Xiaqing,Guo, Peng,et al. Improvement of Endothelial Cell Migration on Nickel-Free Stainless Steel by the Involvement of Epithelial-Mesenchymal Transition[J]. JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING,2018,8(9):1320-1326.
APA	Li, Liming,Liu, Xiaqing,Guo, Peng,Ren, Yibin,&Yang, Ke.(2018).Improvement of Endothelial Cell Migration on Nickel-Free Stainless Steel by the Involvement of Epithelial-Mesenchymal Transition.JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING,8(9),1320-1326.
MLA	Li, Liming,et al."Improvement of Endothelial Cell Migration on Nickel-Free Stainless Steel by the Involvement of Epithelial-Mesenchymal Transition".JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING 8.9(2018):1320-1326.