Influence of chitosan oligosaccharide on the gelling and wound healing properties of injectable hydrogels based on carboxymethyl chitosan/alginate polyelectrolyte complexes

doi:10.1016/j.carbpol.2018.10.067

IMR OpenIR

	Influence of chitosan oligosaccharide on the gelling and wound healing properties of injectable hydrogels based on carboxymethyl chitosan/alginate polyelectrolyte complexes
	Lv, Xiaojie 1,2; Liu, Yunen 3; Song, Subin 1; Tong, Changci 3; Shi, Xiuyun 3; Zhao, Yan 1; Zhang, Jinsong 1; Hou, Mingxiao 3
通讯作者	Zhao, Yan(yanzhao@imr.ac.cn) ; Zhang, Jinsong(jshzhang@imr.ac.cn)
	2019-02-01
发表期刊	CARBOHYDRATE POLYMERS
ISSN	0144-8617
卷号	205 页码:312-321
摘要	In situ injectable hydrogels for wound healing based on carboxymethyl chitosan (CMCS) and alginate were developed in this work. The liquid mixture of CMCS and alginate solutions formed a gel by polyelectrolyte complexation after addition of D-glucono-delta-lactone (GDL), which slowly hydrolyzed and donated protons. When chitosan oligosaccharide (COS) was added into the mixture, a two-stage gelling process occurred. The primary gelling process was similar to that of the hydrogel without COS, while the secondary gelling process appeared about 20 min later, and much stronger hydrogels with storage modulus G' about 1 MPa, 10(4) times higher, were obtained. COS also significantly influenced the microstructure of hydrogels as well as their biological activities. The hydrogels with 0.5% of COS significantly promoted proliferation of human umbilical cord mesenchymal stem cells (HUMSCs). These injectable hydrogels, especially when COS was added, remarkably accelerated the wound healing process in a mouse skin defect model. Microscopic wound analysis showed an increase of the thickness and integrity of epidermal tissue, increased formation of collagen fibers, and enhanced expression of vascular endothelial growth factor as compared to the control group.
关键词	Injectable hydrogels Chitosan oligosaccharide Electrostatic interactions Storage modulus Wound healing
资助者	National Key Research and Development Plan ; National Natural Science Foundation of China
DOI	10.1016/j.carbpol.2018.10.067
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Plan[2016YFB0301502] ; National Natural Science Foundation of China[U1608255]
WOS研究方向	Chemistry ; Polymer Science
WOS类目	Chemistry, Applied ; Chemistry, Organic ; Polymer Science
WOS记录号	WOS:000450093200036
出版者	ELSEVIER SCI LTD
引用统计	被引频次：102[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/130441
专题	中国科学院金属研究所
通讯作者	Zhao, Yan; Zhang, Jinsong
作者单位	1.Chinese Acad Sci, Shenyang Natl Lab Mat Sci, Inst Met Res, Shenyang 110016, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Gen Hosp Shenyang Mil Command, Dept Emergency Med, Shenyang 110016, Peoples R China
推荐引用方式 GB/T 7714	Lv, Xiaojie,Liu, Yunen,Song, Subin,et al. Influence of chitosan oligosaccharide on the gelling and wound healing properties of injectable hydrogels based on carboxymethyl chitosan/alginate polyelectrolyte complexes[J]. CARBOHYDRATE POLYMERS,2019,205:312-321.
APA	Lv, Xiaojie.,Liu, Yunen.,Song, Subin.,Tong, Changci.,Shi, Xiuyun.,...&Hou, Mingxiao.(2019).Influence of chitosan oligosaccharide on the gelling and wound healing properties of injectable hydrogels based on carboxymethyl chitosan/alginate polyelectrolyte complexes.CARBOHYDRATE POLYMERS,205,312-321.
MLA	Lv, Xiaojie,et al."Influence of chitosan oligosaccharide on the gelling and wound healing properties of injectable hydrogels based on carboxymethyl chitosan/alginate polyelectrolyte complexes".CARBOHYDRATE POLYMERS 205(2019):312-321.