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Regulation of osteogenesis and osteoclastogenesis by zoledronic acid loaded on biodegradable magnesium-strontium alloy
Li, Mei1,2; Wan, Peng3; Wang, Weidan4,5; Yang, Ke4; Zhang, Yu2; Han, Yong1
Corresponding AuthorWan, Peng(wanpeng@dgut.edu.cn) ; Zhang, Yu(luck_2001@126.com) ; Han, Yong(yonghan@mail.xjtu.edu.cn)
2019-01-30
Source PublicationSCIENTIFIC REPORTS
ISSN2045-2322
Volume9Pages:12
AbstractInhibiting osteoclasts and osteoclast precursors to reduce bone resorption is an important strategy to treat osteoclast-related diseases, such as peri-prosthetic osteolysis. In this study, our objective was to study the role of zoledronic acid (ZA), as a highly potent and nitrogen-containing bisphosphonate, in promoting osteogenesis and inhibiting osteoclastogenesis properties of magnesium (Mg)-based implants. ZA was chemically associated with calcium phosphate (CaP) deposited on magnesiumstrontium (Mg-Sr) alloy, which was confirmed by the morphological observation, phase composition and drug releasing via SEM, XRD spectrum and High Performance Liquid Chromatography (HPLC), respectively. The in vitro performances indicated that ZA-CaP bilayer coating Mg-Sr alloy could enhance the proliferation and the osteogenic differentiation as well as the mineralization of pre-osteoblasts, however, induce the apoptosis and inhibit the osteoclast differentiation. We further investigated the possible molecular mechanisms by using Quantitative real-time PCR (qRT-PCR) and Western Blotting, and the results showed that ZA-CaP bilayer coating Mg-Sr alloy could regulate the osteogenesis and osteoclastogenesis through the Estrogen Receptor alpha (ER alpha) and NF-kappa B signaling pathway. Moreover, ZA-CaP bilayer coating Mg-Sr alloy could regulate the cross talk of osteoblast-osteoclast and increase the ratio of OPG: RANKL in the co-culture system through OPG/RANKL/RANK signaling pathway, which promoting the balance of bone remodeling process. Therefore, these promising results suggest the potential clinical applications of ZA pretreated Mg-Sr alloys for bone defect repairs and periprosthetical osteolysis due to the excessive differentitation and maturation of osteoclasts.
Funding OrganizationNational Natural Science Foundation of China ; National Key Research and Development Program of China ; Natural Science Foundation of Guangdong Province, China
DOI10.1038/s41598-018-37091-8
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[51371137] ; National Natural Science Foundation of China[81501859] ; National Natural Science Foundation of China[51631007] ; National Natural Science Foundation of China[31500777] ; National Natural Science Foundation of China[81601884] ; National Key Research and Development Program of China[2017YFB0702604] ; National Key Research and Development Program of China[2016YFB0700803] ; Natural Science Foundation of Guangdong Province, China[2015A030312004]
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000457128700010
PublisherNATURE PUBLISHING GROUP
Citation statistics
Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/131160
Collection中国科学院金属研究所
Corresponding AuthorWan, Peng; Zhang, Yu; Han, Yong
Affiliation1.Xi An Jiao Tong Univ, State Key Lab Mech Behav Mat, Xian 710049, Shaanxi, Peoples R China
2.Guangdong Gen Hosp, Guangdong Acad Med Sci, Dept Orthoped, Guangzhou 510080, Guangdong, Peoples R China
3.Dongguan Univ Technol, Sch Mech Engn, Dongguan 523808, Peoples R China
4.Chinese Acad Sci, Inst Met Res, Shenyang 110016, Liaoning, Peoples R China
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Li, Mei,Wan, Peng,Wang, Weidan,et al. Regulation of osteogenesis and osteoclastogenesis by zoledronic acid loaded on biodegradable magnesium-strontium alloy[J]. SCIENTIFIC REPORTS,2019,9:12.
APA Li, Mei,Wan, Peng,Wang, Weidan,Yang, Ke,Zhang, Yu,&Han, Yong.(2019).Regulation of osteogenesis and osteoclastogenesis by zoledronic acid loaded on biodegradable magnesium-strontium alloy.SCIENTIFIC REPORTS,9,12.
MLA Li, Mei,et al."Regulation of osteogenesis and osteoclastogenesis by zoledronic acid loaded on biodegradable magnesium-strontium alloy".SCIENTIFIC REPORTS 9(2019):12.
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