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Effect of Microarc Oxidation-Treated Ti6Al4V Scaffold Following Low-Intensity Pulsed Ultrasound Stimulation on Osteogenic Cells in Vitro
Chen, Jie1,2; Li, Jiongjiong1,2; Hu, Fu1,2; Zou, Qin1,2; Mei, Quanjing1,2; Li, Shujun3; Hao, Yulin3; Hou, Wentao3; Li, Jidong1,2; Li, Yubao1,2; Zuo, Yi1,2
Corresponding AuthorZuo, Yi(zoae@vip.sina.com)
2019-02-01
Source PublicationACS BIOMATERIALS SCIENCE & ENGINEERING
ISSN2373-9878
Volume5Issue:2Pages:572-581
AbstractThe porous Ti6Al4V alloy has emerged to solve the biomechanical mismatch between implant and bone as its tunable mechanical properties. Cell-surface interaction is related to numerous factors-the surface's chemical composition, morphological structure, and external effect. The microarc oxidation (MAO) method was employed in this study to improve the surface properties of scaffolds produced by Electron Beam Melting (EBM), and low-intensity pulse ultrasound (LIPUS) provides physical stimulation for cells in vitro. Although MAO-treated and untreated scaffolds shared the same three-dimensional (3D) structures, the former recreated a more affinity surface than the latter in the 3D room, which could stimulate cell adhesion, proliferation, and differentiation. Therefore, MG63 cells were represented with a stereoscopic cytoskeleton structure on the MAO-treated scaffold as numerous cellular filopodia/lamellipodia with rich extracellular matrix secretion, while flat and sheetlike cells were observed on the untreated scaffold. The expression of ALP, OCN, BMP2, Bmpr1a, and Runx2 were up-regulated by the MAO-treated scaffold; in addition, LIPUS stimulation effectively promoted cell proliferation and osteogenesis differentiation. In the future, the EBM-MAO strategy can be applied to prepare 3D hierarchical macro-/microstructure titanium implants for bone grafts, and LIPUS stimulation can be used as a therapeutic method simultaneously.
Keywordtitanium alloy scaffold EBM-MAO strategy affinity surface stereoscopic cytoskeleton structure LIPUS stimulation cell mineralization
Funding OrganizationChina NSFC Project ; Key Research Program of Frontier Sciences, CAS
DOI10.1021/acsbiomaterials.8b01000
Indexed BySCI
Language英语
Funding ProjectChina NSFC Project[31370971] ; Key Research Program of Frontier Sciences, CAS[QYZDJ-SSW-JSC031-02]
WOS Research AreaMaterials Science
WOS SubjectMaterials Science, Biomaterials
WOS IDWOS:000458937900019
PublisherAMER CHEMICAL SOC
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/131903
Collection中国科学院金属研究所
Corresponding AuthorZuo, Yi
Affiliation1.Sichuan Univ, Res Ctr Nano Biomat, Chengdu 610064, Sichuan, Peoples R China
2.Sichuan Univ, Analyt & Testing Ctr, Chengdu 610064, Sichuan, Peoples R China
3.Chinese Acad Sci, Shenyang Natl Lab Mat Sci, Inst Met Res, 72 Wenhua Rd, Shenyang 110016, Liaoning, Peoples R China
Recommended Citation
GB/T 7714
Chen, Jie,Li, Jiongjiong,Hu, Fu,et al. Effect of Microarc Oxidation-Treated Ti6Al4V Scaffold Following Low-Intensity Pulsed Ultrasound Stimulation on Osteogenic Cells in Vitro[J]. ACS BIOMATERIALS SCIENCE & ENGINEERING,2019,5(2):572-581.
APA Chen, Jie.,Li, Jiongjiong.,Hu, Fu.,Zou, Qin.,Mei, Quanjing.,...&Zuo, Yi.(2019).Effect of Microarc Oxidation-Treated Ti6Al4V Scaffold Following Low-Intensity Pulsed Ultrasound Stimulation on Osteogenic Cells in Vitro.ACS BIOMATERIALS SCIENCE & ENGINEERING,5(2),572-581.
MLA Chen, Jie,et al."Effect of Microarc Oxidation-Treated Ti6Al4V Scaffold Following Low-Intensity Pulsed Ultrasound Stimulation on Osteogenic Cells in Vitro".ACS BIOMATERIALS SCIENCE & ENGINEERING 5.2(2019):572-581.
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