Study of engineered low-modulus Mg/PLLA composites as potential orthopaedic implants: An in vitro and in vivo study

doi:10.1016/j.colsurfb.2018.10.054

IMR OpenIR

	Study of engineered low-modulus Mg/PLLA composites as potential orthopaedic implants: An in vitro and in vivo study
	Yu, Xiaobing 1,2,3; Huang, Wei 1; Zhao, Dewei 1,2; Yang, Ke 3; Tan, Lili 3; Zhang, Xiuzhi 1; Li, Junlei 1; Zhang, Meng 1; Zhang, Shuo 1; Liu, Tao 1; Wu, Baolin 1; Qu, Mingjia 1; Duan, Ruimeng 1; Yuan, Yashuai 1
通讯作者	Zhao, Dewei(zhaodewei2016@163.com) ; Yang, Ke()
	2019-02-01
发表期刊	COLLOIDS AND SURFACES B-BIOINTERFACES
ISSN	0927-7765
卷号	174 页码:280-290
摘要	Low molecular weight poly-lactic acid (PLLA) is a polymer matrix of orthopaedic implants. The PLLA matrix incorporating bioactive magnesium ion (Mg2+) enhances bone regeneration. But the optimal ratio of Mg2+ to PLLA matrix has not been well reported and is worthy of study. We synthesized silane-coated Mg/PLLA composites containing 1%, 2%, 3%, 4% and 5% Mg micro-particles. The mechanical properties, in vitro cytocompatibility, cell viability and osteogenesis differentiation and in vivo performance of silane-coated Mg/PLLA composites were evaluated. These results showed that the bending and tensile strength of PLLA matrix was reduced by incorporation of Mg micro-particles. Mg/PLLA composites with higher Mg micro-particles ratio showed higher Mg2+ leaching rate and pH value in immersion solutions. MC3T3-E1 pre-osteoblasts incubated with Mg/PLLA composites containing higher ratio of Mg micro-particles showed higher cytocompatibility, cell viability, osteogenesis differentiation and migration. In vitro cellular responses showed that MC3T3-E1 preosteoblasts had the highest cell viability at 50 ppm Mg2+. In vivo animal studies showed there was no change in serum Mg 2+ concentration after implanting Mg/PLLA composites comparing with control and the implants of silane-coated Mg/PLLA composites accelerated bone formation. In summary, our study revealed the feasibility of silane-coated Mg/PLLA composites as orthopaedic implants. Silane-coated Mg/PLLA composites with Mg micro-particles ratio of 3% similar to 5% were optimal substitutes for bone regeneration.
关键词	Poly-lactic acid Magnesium Mg/PLLA composite Bone formation Osteogenesis differentiation
资助者	National Natural Science Foundation of China
DOI	10.1016/j.colsurfb.2018.10.054
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[81672139]
WOS研究方向	Biophysics ; Chemistry ; Materials Science
WOS类目	Biophysics ; Chemistry, Physical ; Materials Science, Biomaterials
WOS记录号	WOS:000459840800033
出版者	ELSEVIER SCIENCE BV
引用统计	被引频次：20[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/132125
专题	中国科学院金属研究所
通讯作者	Zhao, Dewei; Yang, Ke
作者单位	1.Dalian Univ, Zhongshan Hosp, Dept Orthopaed, Dalian 116001, Peoples R China 2.Dalian Univ, Zhongshan Hosp, Postdoctoral Workstn, Dalian 116001, Peoples R China 3.Chinese Acad Sci, Inst Met Res, Shenyang 110016, Liaoning, Peoples R China
推荐引用方式 GB/T 7714	Yu, Xiaobing,Huang, Wei,Zhao, Dewei,et al. Study of engineered low-modulus Mg/PLLA composites as potential orthopaedic implants: An in vitro and in vivo study[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2019,174:280-290.
APA	Yu, Xiaobing.,Huang, Wei.,Zhao, Dewei.,Yang, Ke.,Tan, Lili.,...&Yuan, Yashuai.(2019).Study of engineered low-modulus Mg/PLLA composites as potential orthopaedic implants: An in vitro and in vivo study.COLLOIDS AND SURFACES B-BIOINTERFACES,174,280-290.
MLA	Yu, Xiaobing,et al."Study of engineered low-modulus Mg/PLLA composites as potential orthopaedic implants: An in vitro and in vivo study".COLLOIDS AND SURFACES B-BIOINTERFACES 174(2019):280-290.