Light-sheet microscopy in the near-infrared II window

doi:10.1038/s41592-019-0398-7

IMR OpenIR

	Light-sheet microscopy in the near-infrared II window
	Wang, Feifei 1; Wan, Hao 1; Ma, Zhuoran 1; Zhong, Yeteng 1; Sun, Qinchao 1; Tian, Ye 1; Qu, Liangqiong 2,3; Du, Haotian 1; Zhang, Mingxi 4; Li, Lulin 5; Ma, Huilong 6; Luo, Jian 5,7; Liang, Yongye 6; Li, Wen Jung 8,9; Hong, Guosong 10; Liu, Lianqing 8; Dai, Hongjie 1
通讯作者	Dai, Hongjie(hdai@stanford.edu)
	2019-06-01
发表期刊	NATURE METHODS
ISSN	1548-7091
卷号	16 期号:6 页码:545-+
摘要	Non-invasive deep-tissue three-dimensional optical imaging of live mammals with high spatiotemporal resolution is challenging owing to light scattering. We developed near-infrared II (1,000-1,700 nm) light-sheet microscopy with excitation and emission of up to approximately 1,320 nm and 1,700 nm, respectively, for optical sectioning at a penetration depth of approximately 750 mu m through live tissues without invasive surgery and at a depth of approximately 2 mm in glycerol-cleared brain tissues. Near-infrared II light-sheet microscopy in normal and oblique configurations enabled in vivo imaging of live mice through intact tissue, revealing abnormal blood flow and T-cell motion in tumor microcirculation and mapping out programmed-death ligand 1 and programmed cell death protein 1 in tumors with cellular resolution. Three-dimensional imaging through the intact mouse head resolved vascular channels between the skull and brain cortex, and allowed monitoring of recruitment of macrophages and microglia to the traumatic brain injury site.
资助者	National Institutes of Health
DOI	10.1038/s41592-019-0398-7
收录类别	SCI
语种	英语
资助项目	National Institutes of Health[DP1-NS-105737]
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemical Research Methods
WOS记录号	WOS:000469455200024
出版者	NATURE PUBLISHING GROUP
引用统计	被引频次：127[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/133743
专题	中国科学院金属研究所
通讯作者	Dai, Hongjie
作者单位	1.Stanford Univ, Dept Chem & BioX, Stanford, CA 94305 USA 2.Univ N Carolina, Dept Radiol, Chapel Hill, NC USA 3.Univ N Carolina, BRIC, Chapel Hill, NC USA 4.Wuhan Univ Technol, State Key Lab Adv Technol Mat Synth & Proc, Wuhan, Hubei, Peoples R China 5.VA Palo Alto Hlth Care Syst, Palo Alto Vet Inst Res, Palo Alto, CA USA 6.South Univ Sci & Technol China, Dept Mat Sci & Engn, Shenzhen, Peoples R China 7.Stanford Univ, Sch Med, Dept Neurol & Neurol Sci, Stanford, CA 94305 USA 8.Chinese Acad Sci, Shenyang Inst Automat, State Key Lab Robot, Shenyang, Liaoning, Peoples R China 9.City Univ Hong Kong, Dept Mech Engn, Hong Kong, Peoples R China 10.Stanford Univ, Dept Mat Sci & Engn, Stanford, CA 94305 USA
推荐引用方式 GB/T 7714	Wang, Feifei,Wan, Hao,Ma, Zhuoran,et al. Light-sheet microscopy in the near-infrared II window[J]. NATURE METHODS,2019,16(6):545-+.
APA	Wang, Feifei.,Wan, Hao.,Ma, Zhuoran.,Zhong, Yeteng.,Sun, Qinchao.,...&Dai, Hongjie.(2019).Light-sheet microscopy in the near-infrared II window.NATURE METHODS,16(6),545-+.
MLA	Wang, Feifei,et al."Light-sheet microscopy in the near-infrared II window".NATURE METHODS 16.6(2019):545-+.