Biofunctional magnesium coated Ti6Al4V scaffold enhances osteogenesis and angiogenesis in vitro and in vivo for orthopedic application<bold> </bold>

doi:10.1016/j.bioactmat.2020.04.019

IMR OpenIR

	Biofunctional magnesium coated Ti6Al4V scaffold enhances osteogenesis and angiogenesis in vitro and in vivo for orthopedic application
	Gao, Peng 1,2,3; Fan, Bo 1; Yu, Xiaoming 4,5; Liu, Wenwen 1; Wu, Jie 1; Shi, Lei 1; Yang, Di 1; Tan, Lili 4; Wan, Peng 4; Hao, Yulin 4; Li, Shujun 4; Hou, Wentao 4; Yang, Ke 4; Li, Xiaokang 1; Guo, Zheng 1
Corresponding Author	Yang, Ke(kyang@imr.ac.cn) ; Li, Xiaokang(lxkfmmu@163.com) ; Guo, Zheng(guozheng@fmmu.edu.cn)
	2020-09-01
Source Publication	BIOACTIVE MATERIALS
Volume	5 Issue:3 Pages:680-693
Abstract	The insufficient osteogenesis and osseointegration of porous titanium based scaffold limit its further application. Early angiogenesis is important for scaffold survival. It is necessary to develop a multifunctional surface on titanium scaffold with both osteogenic and angiogenic properties. In this study, a biofunctional magnesium coating is deposited on porous Ti6Al4V scaffold. For osseointegration and osteogenesis analysis, in vitro studies reveal that magnesium-coated Ti6Al4V co-culture with MC3T3-E1 cells can improve cell proliferation, adhesion, extracellular matrix (ECM) mineralization and ALP activity compared with bare Ti6Al4V cocultivation. Additionally, MC3T3-E1 cells cultured with magnesium-coated Ti6Al4V show significantly higher osteogenesis-related genes expression. In vivo studies including fluorochrome labeling, micro-computerized tomography and histological examination of magnesium-coated Ti6Al4V scaffold reveal that new bone regeneration is significantly increased in rabbits after implantation. For angiogenesis studies, magnesium-coated Ti6Al4V improve HUVECs proliferation, adhesion, tube formation, wound-healing and Transwell abilities. HUVECs cultured with magnesium-coated Ti6Al4V display significantly higher angiogenesis-related genes (HIF-1 alpha and VEGF) expression. Microangiography analysis reveal that magnesium-coated Ti6Al4V scaffold can significantly enhance the blood vessel formation. This study enlarges the application scope of magnesium and provides an optional choice to the conventional porous Ti6Al4V scaffold with enhanced osteogenesis and angiogenesis for further orthopedic applications.
Keyword	Porous Ti6Al4V scafflod Surface modification Magnesium coating Osteogenesis Angiogenesis
Funding Organization	National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of Youth Fund
DOI	10.1016/j.bioactmat.2020.04.019
Indexed By	SCI
Language	英语
Funding Project	National Key Research and Development Program of China[2017YFC1104901] ; National Natural Science Foundation of China[51871239] ; National Natural Science Foundation of China[51771227] ; National Natural Science Foundation of Youth Fund[51501223]
WOS Research Area	Engineering ; Materials Science
WOS Subject	Engineering, Biomedical ; Materials Science, Biomaterials
WOS ID	WOS:000546576100023
Publisher	KEAI PUBLISHING LTD
Citation statistics	Cited Times:70[WOS] [WOS Record] [Related Records in WOS]
Document Type	期刊论文
Identifier	http://ir.imr.ac.cn/handle/321006/139590
Collection	中国科学院金属研究所
Corresponding Author	Yang, Ke; Li, Xiaokang; Guo, Zheng
Affiliation	1.Fourth Mil Med Univ, Xijing Hosp, Dept Orthoped, Xian 710032, Peoples R China 2.Hunan Normal Univ, Dept Joint Surg & Sports Med, Hunan Prov Peoples Hosp, Changsha 410016, Peoples R China 3.Hunan Normal Univ, Affiliated Hosp 1, Changsha 410016, Peoples R China 4.Chinese Acad Sci, Inst Met Res, Shenyang 110016, Peoples R China 5.Shenyang Ligong Univ, Sch Mat Sci & Engn, Shenyang 110159, Peoples R China
Recommended Citation GB/T 7714	Gao, Peng,Fan, Bo,Yu, Xiaoming,et al. Biofunctional magnesium coated Ti6Al4V scaffold enhances osteogenesis and angiogenesis in vitro and in vivo for orthopedic application [J]. BIOACTIVE MATERIALS,2020,5(3):680-693.
APA	Gao, Peng.,Fan, Bo.,Yu, Xiaoming.,Liu, Wenwen.,Wu, Jie.,...&Guo, Zheng.(2020).Biofunctional magnesium coated Ti6Al4V scaffold enhances osteogenesis and angiogenesis in vitro and in vivo for orthopedic application .BIOACTIVE MATERIALS,5(3),680-693.
MLA	Gao, Peng,et al."Biofunctional magnesium coated Ti6Al4V scaffold enhances osteogenesis and angiogenesis in vitro and in vivo for orthopedic application ".BIOACTIVE MATERIALS 5.3(2020):680-693.

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