Bioinformatics analysis and identification of circular RNAs promoting the osteogenic differentiation of human bone marrow mesenchymal stem cells on titanium treated by surface mechanical attrition

doi:10.7717/peerj.9292

IMR OpenIR

	Bioinformatics analysis and identification of circular RNAs promoting the osteogenic differentiation of human bone marrow mesenchymal stem cells on titanium treated by surface mechanical attrition
	Zhu, Shanshan 1; Zhu, Yuhe 1; Wang, Zhenbo 2; Liang, Chen 2; Cao, Nanjue 3; Yan, Ming 1; Gao, Fei 1; Liu, Jie 4; Wang, Wei 1
通讯作者	Liu, Jie(lj6152003@163.com) ; Wang, Wei(wwang75@cmu.edu.cn)
	2020-07-13
发表期刊	PEERJ
ISSN	2167-8359
卷号	8 页码:22
摘要	Background: To analyze and identify the circular RNAs (circRNAs) involved in promoting the osteogenic differentiation of human bone mesenchymal stem cells (hBMSCs) on titanium by surface mechanical attrition treatment (SMAT). Methods: The experimental material was SMAT titanium and the control material was annealed titanium. Cell Counting Kits-8 (CCK-8) was used to detect the proliferation of hBMSCs, and alkaline phosphatase (ALP) activity and alizarin red staining were used to detect the osteogenic differentiation of hBMSCs on the sample surfaces. The bioinformatics prediction software miwalk3.0 was used to construct competing endogenous RNA (ceRNA) networks by predicting circRNAs with osteogenesis-related messenger RNAs (mRNAs) and microRNAs (miRNAs). The circRNAs located at the key positions in the networks were selected and analyzed by quantitative real-time PCR (QRT-PCR). Results: Compared with annealed titanium, SMAT titanium could promote the proliferation and osteogenic differentiation of hBMSCs. The total number of predicted circRNAs was 51. Among these, 30 circRNAs and 8 miRNAs constituted 6 ceRNA networks. Circ-LTBP2 was selected for verification. QRT-PCR results showed that the expression levels of hsa_circ_0032599, hsa_circ_0032600 and hsa_circ_0032601 were upregulated in the experimental group compared with those in the control group; the differential expression of hsa_circ_0032600 was the most obvious and statistically significant, with a fold change (FC) = 4.25 +/- 1.60, p-values (p) <0.05.
关键词	Surface mechanical attrition treatment Human bone marrow mesenchymal stem cells Biological information prediction Osteogenic differentiation circRNA
资助者	National Natural Science Foundation of China ; Liaoning Province, Colleges and Universities Basic Research Project ; Liaoning Provincial Key Research Plan Guidance Project ; Liaoning Provincial Natural Science Foundation Guidance Project ; Shenyang Major Scientific and Technological Innovation Research and Development Plan ; Shenyang Young and Middle-aged Technological Innovation Talent Plan ; Shenyang National Laboratory for Materials Science ; Second Batch of Medical Education Scientific Research Projects of the 13th Five-Year Plan of China Medical University
DOI	10.7717/peerj.9292
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[81970980] ; Liaoning Province, Colleges and Universities Basic Research Project[LFWK201717] ; Liaoning Provincial Key Research Plan Guidance Project[2018225078] ; Liaoning Provincial Natural Science Foundation Guidance Project[2019-ZD-0749] ; Shenyang Major Scientific and Technological Innovation Research and Development Plan[19-112-4-027] ; Shenyang Young and Middle-aged Technological Innovation Talent Plan[RC200060] ; Shenyang National Laboratory for Materials Science[2015RP04] ; Second Batch of Medical Education Scientific Research Projects of the 13th Five-Year Plan of China Medical University[YDJK2018017]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000547882300001
出版者	PEERJ INC
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/139657
专题	中国科学院金属研究所
通讯作者	Liu, Jie; Wang, Wei
作者单位	1.China Med Univ, Sch & Hosp Stomatol, Liaoning Prov Key Lab Oral Dis, Shenyang, Liaoning, Peoples R China 2.Chinese Acad Sci, Inst Met Res, Shenyang Natl Lab Mat Sci, Shenyang, Liaoning, Peoples R China 3.Zhejiang Univ, Affiliated Hosp 4, Sch Med, Yiwu, Zhejiang, Peoples R China 4.China Med Univ, Dept Sci Expt Ctr 1, Shenyang, Liaoning, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Shanshan,Zhu, Yuhe,Wang, Zhenbo,et al. Bioinformatics analysis and identification of circular RNAs promoting the osteogenic differentiation of human bone marrow mesenchymal stem cells on titanium treated by surface mechanical attrition[J]. PEERJ,2020,8:22.
APA	Zhu, Shanshan.,Zhu, Yuhe.,Wang, Zhenbo.,Liang, Chen.,Cao, Nanjue.,...&Wang, Wei.(2020).Bioinformatics analysis and identification of circular RNAs promoting the osteogenic differentiation of human bone marrow mesenchymal stem cells on titanium treated by surface mechanical attrition.PEERJ,8,22.
MLA	Zhu, Shanshan,et al."Bioinformatics analysis and identification of circular RNAs promoting the osteogenic differentiation of human bone marrow mesenchymal stem cells on titanium treated by surface mechanical attrition".PEERJ 8(2020):22.