Cytotoxicity of Ti-6Al-4V-5Cu Alloy to MC3T3-E1 Cells

doi:10.1007/s40195-020-01158-1

IMR OpenIR

	Cytotoxicity of Ti-6Al-4V-5Cu Alloy to MC3T3-E1 Cells
	Zhang, Ji; Ren, Ling; Yang, Ke
通讯作者	Yang, Ke(kyang@imr.ac.cn)
	2020-11-11
发表期刊	ACTA METALLURGICA SINICA-ENGLISH LETTERS
ISSN	1006-7191
页码	7
摘要	The aim of this work was to study the cytotoxicity of Ti-6Al-4V-5Cu (TC4-Cu) alloy for dental applications and evaluate the cell viability of different fabricated TC4-Cu alloys in contact with MC3T3-E1 cells in vitro. Ti-6Al-4V (TC4) alloy was used as a negative control to evaluate the cytotoxicity level of TC4-Cu alloy so as to provide basic support for the dental clinical application. Control group TC4 and experimental group TC4-Cu with different fabrications were incubated in the cell culture medium. The absorbance value of mouse osteoblast MC3T3-E1 cells was detected by CCK-8 assay after 1, 3 and 7 days, respectively. The relative proliferation rate of cells was calculated, and then the toxicity level was valued for each group. Cell morphology on the surface was also studied by observing the cytoskeleton through F-actin filament staining. The experimental results showed that the absorbance values for the experimental and the negative control groups were not same at different time points. Compared with different fabricated TC4-Cu alloys, the annealing-TC4-Cu alloy showed much better biocompatibility. The mouse osteoblast MC3T3-E1 cells cultured with annealing-TC4-Cu, rolling-TC4-Cu and solution + aging-TC4-Cu have no toxic effects, and these alloys could promote the proliferation of mouse osteoblasts.
关键词	Cytotoxicity Ti– 6Al– 4V– 5Cu alloy Fabrication Biocompatibility
资助者	National Natural Science Foundation of China
DOI	10.1007/s40195-020-01158-1
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[51631009]
WOS研究方向	Metallurgy & Metallurgical Engineering
WOS类目	Metallurgy & Metallurgical Engineering
WOS记录号	WOS:000588588000003
出版者	CHINESE ACAD SCIENCES, INST METAL RESEARCH
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/141316
专题	中国科学院金属研究所
通讯作者	Yang, Ke
作者单位	Chinese Acad Sci, Inst Met Res, Shenyang 110016, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Ji,Ren, Ling,Yang, Ke. Cytotoxicity of Ti-6Al-4V-5Cu Alloy to MC3T3-E1 Cells[J]. ACTA METALLURGICA SINICA-ENGLISH LETTERS,2020:7.
APA	Zhang, Ji,Ren, Ling,&Yang, Ke.(2020).Cytotoxicity of Ti-6Al-4V-5Cu Alloy to MC3T3-E1 Cells.ACTA METALLURGICA SINICA-ENGLISH LETTERS,7.
MLA	Zhang, Ji,et al."Cytotoxicity of Ti-6Al-4V-5Cu Alloy to MC3T3-E1 Cells".ACTA METALLURGICA SINICA-ENGLISH LETTERS (2020):7.