Effects of gentamicin on choline acetyltransferase expression in paraolivary nucleus neurons of guinea pigs

IMR OpenIR

	Effects of gentamicin on choline acetyltransferase expression in paraolivary nucleus neurons of guinea pigs
其他题名	Effects of gentamicin on choline acetyltransferase expression in paraolivary nucleus neurons of guinea pigs
	Zhao Mingguang; Wang Xiaochen; Liang Yong; Xie Peng; Guo Xuejun; Li Jinjiang; Wang Wei
	2008
发表期刊	NEURAL REGENERATION RESEARCH
ISSN	1673-5374
卷号	3 期号:11 页码:1229-1232
摘要	BACKGROUND: It is generally accepted that gentamicin can damage the cochlear nerve and acoustic nerve. In recent years, scholars have focused on neuronal changes and neurochemical information in the brainstem primary auditory center.
其他摘要	BACKGROUND: It is generally accepted that gentamicin can damage the cochlear nerve and acoustic nerve. In recent years,scholars have focused on neuronal changes and neurochemical information in the brainstem primary auditory center. OBJECTIVE: To explore morphological changes of choline acetyltransferase (ChAT)-positive neurons in the paraolivary nucleus (PON) of guinea pigs,and the effect on hearing following gentamicin injection. DESIGN,TIME AND SETTING: Randomized grouping and morphological observational study was performed at Animal Experimental Center of General Hospital of Shenyang Military Area Command of Chinese PLA from January to August 2007. MATERIALS: A total of 48 healthy guinea pigs were randomly divided into model (n=40) and control (n= 8) groups. The model group was divided into five subgroups at five time points of 1 and 3 days,1,2,and 3 weeks. METHODS: Guinea pigs in the model group were intraperitoneally injected with gentamicin,and those in the control group were in traperitoneally injected with the same volume of saline. MAIN OUTCOME MEASURES: Auditory brainstem-evoked potential was used to record auditory threshold; distribution and morphological changes of ChAT-positive neurons in the PON were observed with immunohistochemistry; section area and gray value of ChAT-positive neurons were measured with Quantimet 570 image-analyzing system. RESULTS: ChAT-positive neurons were diffusedly distributed in the PON. The majority was composed of large,round cells,with positive neurites that could be clearly observed. Following gentamicin injection,the positive neurons displayed an irregular outline,and their neurites began to shorten and disappear. The gray value increased with prolonged gentamicin administration (P<0.05). In addition,the somatic cross-sectional area was enlarged in the model group at 1 and 3 days after injection (P<0.05),whereas cell number significantly decreased at three weeks after injection (P<0.05). Starting at 3-4 days,behavioral features and auditory degrees became gradually aggravated with prolonged gentamicin administration (P<0.05). CONCLUSION: Gentamicin damaged ChAT-positive neurons in the PON,and long-term gentamicin treatment aggravated hearing impairment.
关键词	RAT COCHLEAR NUCLEUS OUTER HAIR-CELLS AUDITORY-SYSTEM ACETYLCHOLINE RECEPTOR INNERVATION ACTIVATION PLASTICITY MICE MAINTENANCE choline acetyltransferase gentamicin guinea pigs paraolivary nucleus
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:3483438
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/141701
专题	中国科学院金属研究所
作者单位	中国科学院金属研究所
推荐引用方式 GB/T 7714	Zhao Mingguang,Wang Xiaochen,Liang Yong,et al. Effects of gentamicin on choline acetyltransferase expression in paraolivary nucleus neurons of guinea pigs[J]. NEURAL REGENERATION RESEARCH,2008,3(11):1229-1232.
APA	Zhao Mingguang.,Wang Xiaochen.,Liang Yong.,Xie Peng.,Guo Xuejun.,...&Wang Wei.(2008).Effects of gentamicin on choline acetyltransferase expression in paraolivary nucleus neurons of guinea pigs.NEURAL REGENERATION RESEARCH,3(11),1229-1232.
MLA	Zhao Mingguang,et al."Effects of gentamicin on choline acetyltransferase expression in paraolivary nucleus neurons of guinea pigs".NEURAL REGENERATION RESEARCH 3.11(2008):1229-1232.