IMR OpenIR
Effects of gentamicin on choline acetyltransferase expression in paraolivary nucleus neurons of guinea pigs
Alternative TitleEffects of gentamicin on choline acetyltransferase expression in paraolivary nucleus neurons of guinea pigs
Zhao Mingguang; Wang Xiaochen; Liang Yong; Xie Peng; Guo Xuejun; Li Jinjiang; Wang Wei
2008
Source PublicationNEURAL REGENERATION RESEARCH
ISSN1673-5374
Volume3Issue:11Pages:1229-1232
AbstractBACKGROUND: It is generally accepted that gentamicin can damage the cochlear nerve and acoustic nerve. In recent years, scholars have focused on neuronal changes and neurochemical information in the brainstem primary auditory center.
Other AbstractBACKGROUND: It is generally accepted that gentamicin can damage the cochlear nerve and acoustic nerve. In recent years,scholars have focused on neuronal changes and neurochemical information in the brainstem primary auditory center. OBJECTIVE: To explore morphological changes of choline acetyltransferase (ChAT)-positive neurons in the paraolivary nucleus (PON) of guinea pigs,and the effect on hearing following gentamicin injection. DESIGN,TIME AND SETTING: Randomized grouping and morphological observational study was performed at Animal Experimental Center of General Hospital of Shenyang Military Area Command of Chinese PLA from January to August 2007. MATERIALS: A total of 48 healthy guinea pigs were randomly divided into model (n=40) and control (n= 8) groups. The model group was divided into five subgroups at five time points of 1 and 3 days,1,2,and 3 weeks. METHODS: Guinea pigs in the model group were intraperitoneally injected with gentamicin,and those in the control group were in traperitoneally injected with the same volume of saline. MAIN OUTCOME MEASURES: Auditory brainstem-evoked potential was used to record auditory threshold; distribution and morphological changes of ChAT-positive neurons in the PON were observed with immunohistochemistry; section area and gray value of ChAT-positive neurons were measured with Quantimet 570 image-analyzing system. RESULTS: ChAT-positive neurons were diffusedly distributed in the PON. The majority was composed of large,round cells,with positive neurites that could be clearly observed. Following gentamicin injection,the positive neurons displayed an irregular outline,and their neurites began to shorten and disappear. The gray value increased with prolonged gentamicin administration (P<0.05). In addition,the somatic cross-sectional area was enlarged in the model group at 1 and 3 days after injection (P<0.05),whereas cell number significantly decreased at three weeks after injection (P<0.05). Starting at 3-4 days,behavioral features and auditory degrees became gradually aggravated with prolonged gentamicin administration (P<0.05). CONCLUSION: Gentamicin damaged ChAT-positive neurons in the PON,and long-term gentamicin treatment aggravated hearing impairment.
KeywordRAT COCHLEAR NUCLEUS OUTER HAIR-CELLS AUDITORY-SYSTEM ACETYLCHOLINE RECEPTOR INNERVATION ACTIVATION PLASTICITY MICE MAINTENANCE choline acetyltransferase gentamicin guinea pigs paraolivary nucleus
Indexed ByCSCD
Language英语
CSCD IDCSCD:3483438
Citation statistics
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/141704
Collection中国科学院金属研究所
Affiliation中国科学院金属研究所
Recommended Citation
GB/T 7714
Zhao Mingguang,Wang Xiaochen,Liang Yong,et al. Effects of gentamicin on choline acetyltransferase expression in paraolivary nucleus neurons of guinea pigs[J]. NEURAL REGENERATION RESEARCH,2008,3(11):1229-1232.
APA Zhao Mingguang.,Wang Xiaochen.,Liang Yong.,Xie Peng.,Guo Xuejun.,...&Wang Wei.(2008).Effects of gentamicin on choline acetyltransferase expression in paraolivary nucleus neurons of guinea pigs.NEURAL REGENERATION RESEARCH,3(11),1229-1232.
MLA Zhao Mingguang,et al."Effects of gentamicin on choline acetyltransferase expression in paraolivary nucleus neurons of guinea pigs".NEURAL REGENERATION RESEARCH 3.11(2008):1229-1232.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhao Mingguang]'s Articles
[Wang Xiaochen]'s Articles
[Liang Yong]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhao Mingguang]'s Articles
[Wang Xiaochen]'s Articles
[Liang Yong]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhao Mingguang]'s Articles
[Wang Xiaochen]'s Articles
[Liang Yong]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.