| In vitro differentiation of adipose-derived stem cells and bone marrow-derived stromal stem cells into neuronal-like cells | |
| Alternative Title | In vitro differentiation of adipose-derived stem cells and bone marrow-derived stromal stem cells into neuronal-like cells |
| Zhou Jin; Tian Guoping; Wang Jinge; Cong Xuefeng; Wu Xingkai; Zhang Siyang; Li Li; Xu Bing; Zhu Feng; Luo Xuedan; Han Jian; Hu Fengjie | |
| 2011 | |
| Source Publication | NEURAL REGENERATION RESEARCH
 |
| ISSN | 1673-5374 |
| Volume | 6Issue:19Pages:1467-1472 |
| Abstract | Adipose-derived stem cells and bone marrow-derived stromal stem cells were co-cultured with untreated or A beta(1-40)-treated PC12 cells, or grown in supernatant derived from untreated or A beta(1-40)-treated PC12 cells. Analysis by western blot and quantitative real-time PCR showed that protein levels of Nanog, Oct4, and Sox2, and mRNA levels of miR/125a/3p were decreased, while expression of insulin-like growth factor-2 and neuron specific enolase was increased. In comparison, the generation of neuron specific enolase-positive cells was most successful when adipose-derived stem cells were co-cultured with A beta(1-40)-treated PC12 cells. Our results demonstrate that adipose-derived stem cells and bone marrow-derived stromal stem cells exhibit trends of neuronal-like cell differentiation after co-culture with A beta(1-40)-treated PC12 cells. This process may relate to a downregulation of miR-125a-3p mRNA expression and increased levels of insulin-like growth factor-2 expression. |
| Other Abstract | Adipose-derived stem cells and bone marrow-derived stromal stem cells were co-cultured with untreated or Aβ1-40-treated PC12 cells,or grown in supernatant derived from untreated or Aβ1-40-treated PC12 cells.Analysis by western blot and quantitative real-time PCR showed that protein levels of Nanog,Oct4,and Sox2,and mRNA levels of miR/125a/3p were decreased,while expression of insulin-like growth factor-2 and neuron specific enolase was increased.In comparison,the generation of neuron specific enolase-positive cells was most successful when adipose-derived stem cells were co-cultured with Aβ1-40-treated PC12 cells.Our results demonstrate that adipose-derived stem cells and bone marrow-derived stromal stem cells exhibit trends of neuronal-like cell differentiation after co-culture with Aβ1-40-treated PC12 cells.This process may relate to a downregulation of miR-125a-3p mRNA expression and increased levels of insulin-like growth factor-2 expression. |
| Keyword | SELF-RENEWAL EXPRESSION TISSUE NANOG SOX2 PLURIPOTENCY MICRORNAS THERAPY LINEAGE OCT4 adipose-derived stem cells bone marrow-derived stromal stem cells differentiation neuron miR-125a-3p neural regeneration |
| Indexed By | CSCD |
| Language | 英语 |
| Funding Project | [Shenyang Science and Technology Bureau] |
| CSCD ID | CSCD:4310629 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.imr.ac.cn/handle/321006/141721 |
| Collection | 中国科学院金属研究所 |
| Affiliation | 中国科学院金属研究所 |
| Recommended Citation GB/T 7714 | Zhou Jin,Tian Guoping,Wang Jinge,et al. In vitro differentiation of adipose-derived stem cells and bone marrow-derived stromal stem cells into neuronal-like cells[J]. NEURAL REGENERATION RESEARCH,2011,6(19):1467-1472. |
| APA | Zhou Jin.,Tian Guoping.,Wang Jinge.,Cong Xuefeng.,Wu Xingkai.,...&Hu Fengjie.(2011).In vitro differentiation of adipose-derived stem cells and bone marrow-derived stromal stem cells into neuronal-like cells.NEURAL REGENERATION RESEARCH,6(19),1467-1472. |
| MLA | Zhou Jin,et al."In vitro differentiation of adipose-derived stem cells and bone marrow-derived stromal stem cells into neuronal-like cells".NEURAL REGENERATION RESEARCH 6.19(2011):1467-1472. |
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