In vitro differentiation of adipose-derived stem cells and bone marrow-derived stromal stem cells into neuronal-like cells

IMR OpenIR

	In vitro differentiation of adipose-derived stem cells and bone marrow-derived stromal stem cells into neuronal-like cells
其他题名	In vitro differentiation of adipose-derived stem cells and bone marrow-derived stromal stem cells into neuronal-like cells
	Zhou Jin; Tian Guoping; Wang Jinge; Cong Xuefeng; Wu Xingkai; Zhang Siyang; Li Li; Xu Bing; Zhu Feng; Luo Xuedan; Han Jian; Hu Fengjie
	2011
发表期刊	NEURAL REGENERATION RESEARCH
ISSN	1673-5374
卷号	6 期号:19 页码:1467-1472
摘要	Adipose-derived stem cells and bone marrow-derived stromal stem cells were co-cultured with untreated or A beta(1-40)-treated PC12 cells, or grown in supernatant derived from untreated or A beta(1-40)-treated PC12 cells. Analysis by western blot and quantitative real-time PCR showed that protein levels of Nanog, Oct4, and Sox2, and mRNA levels of miR/125a/3p were decreased, while expression of insulin-like growth factor-2 and neuron specific enolase was increased. In comparison, the generation of neuron specific enolase-positive cells was most successful when adipose-derived stem cells were co-cultured with A beta(1-40)-treated PC12 cells. Our results demonstrate that adipose-derived stem cells and bone marrow-derived stromal stem cells exhibit trends of neuronal-like cell differentiation after co-culture with A beta(1-40)-treated PC12 cells. This process may relate to a downregulation of miR-125a-3p mRNA expression and increased levels of insulin-like growth factor-2 expression.
其他摘要	Adipose-derived stem cells and bone marrow-derived stromal stem cells were co-cultured with untreated or Aβ1-40-treated PC12 cells,or grown in supernatant derived from untreated or Aβ1-40-treated PC12 cells.Analysis by western blot and quantitative real-time PCR showed that protein levels of Nanog,Oct4,and Sox2,and mRNA levels of miR/125a/3p were decreased,while expression of insulin-like growth factor-2 and neuron specific enolase was increased.In comparison,the generation of neuron specific enolase-positive cells was most successful when adipose-derived stem cells were co-cultured with Aβ1-40-treated PC12 cells.Our results demonstrate that adipose-derived stem cells and bone marrow-derived stromal stem cells exhibit trends of neuronal-like cell differentiation after co-culture with Aβ1-40-treated PC12 cells.This process may relate to a downregulation of miR-125a-3p mRNA expression and increased levels of insulin-like growth factor-2 expression.
关键词	SELF-RENEWAL EXPRESSION TISSUE NANOG SOX2 PLURIPOTENCY MICRORNAS THERAPY LINEAGE OCT4 adipose-derived stem cells bone marrow-derived stromal stem cells differentiation neuron miR-125a-3p neural regeneration
收录类别	CSCD
语种	英语
资助项目	[Shenyang Science and Technology Bureau]
CSCD记录号	CSCD:4310629
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/141721
专题	中国科学院金属研究所
作者单位	中国科学院金属研究所
推荐引用方式 GB/T 7714	Zhou Jin,Tian Guoping,Wang Jinge,et al. In vitro differentiation of adipose-derived stem cells and bone marrow-derived stromal stem cells into neuronal-like cells[J]. NEURAL REGENERATION RESEARCH,2011,6(19):1467-1472.
APA	Zhou Jin.,Tian Guoping.,Wang Jinge.,Cong Xuefeng.,Wu Xingkai.,...&Hu Fengjie.(2011).In vitro differentiation of adipose-derived stem cells and bone marrow-derived stromal stem cells into neuronal-like cells.NEURAL REGENERATION RESEARCH,6(19),1467-1472.
MLA	Zhou Jin,et al."In vitro differentiation of adipose-derived stem cells and bone marrow-derived stromal stem cells into neuronal-like cells".NEURAL REGENERATION RESEARCH 6.19(2011):1467-1472.