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Construction of EGFP-tagged rBCG of E.tenella and distribution in chickens
Alternative TitleConstruction of EGFP-tagged rBCG of E.tenella and distribution in chickens
Wang QiuYue1; Li JianHua1; Zhang XiChen1; Liu ChengWu2; Cao LiLi1; Ren KeYan3; Gong PengTao1; Cai YaNan1
2009
Source PublicationSCIENCE IN CHINA SERIES C-LIFE SCIENCES
ISSN1006-9305
Volume52Issue:3Pages:278-283
AbstractChicken coccidiosis is a major parasitic disease with substantial economic burden to the poultry industry. Enhanced green fluorescent protein (EGFP) tagged recombinant Bacille Calmette-Guerin (rBCG), as a fusion protein with coccidian rhomboid antigen was constructed to track rBCG in vivo in chickens in this study. Immunization of chickens with one dose of rBCG pMV361-Rho/EGFP induced humoral immune response. The colonization of rBCG in liver, spleen, lung, kidney and caecum was observed by laser confocal microscopy. Real-time quantitative RT-PCR showed a rise expression level of rhomboid protein on the 7th day and a peak on the 14th day and disappearance on the 28th day after immunization. These results have significant implications for the development of rBCG vaccines against avian coccidiosis.
Other AbstractChicken coccidiosis is a major parasitic disease with substantial economic burden to the poultry in-dustry. Enhanced green fluorescent protein (EGFP) tagged recombinant Bacille Calmette-Guerin (rBCG), as a fusion protein with coccidian rhomboid antigen was constructed to track rBCG in vivo in chickens in this study. Immunization of chickens with one dose of rBCG pMV361-Rho/EGFP induced humoral immune response. The colonization of rBCG in liver, spleen, lung, kidney and caecum was observed by laser confocal microscopy. Real-time quantitative RT-PCR showed s rise expression level of rhomboid protein on the 7th day and a peak on the 14th day and disappearance on the 28th day after immunization. These results have significant implications for the development of rBCG vaccines against avian coccidiosis.
KeywordIMMUNE-RESPONSES ANTIGEN DELIVERY EIMERIA-TENELLA VACCINE VECTORS BCG COCCIDIOSIS PROTECTION CHALLENGE IMMUNIZATION TOXOPLASMA coccidiosis rBCG EGFP rhomboid laser confocal microscopy real-time RT-PCR
Indexed ByCSCD
Language英语
CSCD IDCSCD:3590941
Citation statistics
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/141857
Collection中国科学院金属研究所
Affiliation1.吉林大学
2.中国科学院金属研究所
3.中山大学
Recommended Citation
GB/T 7714
Wang QiuYue,Li JianHua,Zhang XiChen,et al. Construction of EGFP-tagged rBCG of E.tenella and distribution in chickens[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,2009,52(3):278-283.
APA Wang QiuYue.,Li JianHua.,Zhang XiChen.,Liu ChengWu.,Cao LiLi.,...&Cai YaNan.(2009).Construction of EGFP-tagged rBCG of E.tenella and distribution in chickens.SCIENCE IN CHINA SERIES C-LIFE SCIENCES,52(3),278-283.
MLA Wang QiuYue,et al."Construction of EGFP-tagged rBCG of E.tenella and distribution in chickens".SCIENCE IN CHINA SERIES C-LIFE SCIENCES 52.3(2009):278-283.
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