Alpha-actinin expression at different differentiating time points from temporal lobe cerebral cortex neural stem cells to neuron-like cells using energy dispersive X-ray analysis

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	Alpha-actinin expression at different differentiating time points from temporal lobe cerebral cortex neural stem cells to neuron-like cells using energy dispersive X-ray analysis
其他题名	Alpha-actinin expression at different differentiating time points from temporal lobe cerebral cortex neural stem cells to neuron-like cells using energy dispersive X-ray analysis
	Yu Bo; Li Hua; Du Zhe; Hong Yang; Sang Meng; Shi Yuxiu
	2009
发表期刊	NEURAL REGENERATION RESEARCH
ISSN	1673-5374
卷号	4 期号:4 页码:265-270
摘要	BACKGROUND: Alpha-actinin (alpha-actinin) plays a key role in neuronal growth cone migration during directional differentiation from neural stem cells (NSCs) to neurons.
其他摘要	BACKGROUND: Alpha-actinin (α-actinin) plays a key role in neuronal growth cone migration during directional differentiation from neural stem cells (NSCs) to neurons.OBJECTIVE: To detect in situ microdistribution and quantitative expression of α-actinin during directional differentiation of NSCs to neurons in the temporal lobe cerebral cortex of neonatal rats.DESIGN, TIME AND SETTING: Between January 2006 and December 2008, culture and directional differentiation of NSCs were performed at Department of Histology and Embryology, Preclinical Medical College, China Medical University. Immune electron microscopy was performed at Department of Histology and Embryology and Department of Electron Micrology, Preclinical Medical College, China Medical University. Spectrum analysis was performed at Laboratory of Electron Microscopy, Mental Research Institute, Chinese Academy of Sciences.MATERIALS: Basic fibroblast growth factor, epidermal growth factor, brain-derived nerve growth factor, type-1 insulin like growth factor, and α-actinin antibody were provided by Gibco BRL, USA; rabbit-anti-rat nestin monoclonal antibody, rabbit-anti-rat neuron specific enolase polyclonal antibody, and EDAX-9100 energy dispersive X-ray analysis were provided by PHILIPS Company, Netherlands.METHODS: NSCs, following primary and passage culture, were differentiated with serum culture medium (DMEM/F_(12)+10% fetal bovine serum+2ng/mL brain-derived nerve growth factor+2 ng/mL type-1 insulin like growth factor).MAIN OUTCOME MEASURES: Expression of α-actinin in neuron-like cells was quantitatively and qualitatively detected with immunocytochemistry using energy dispersive X-ray analysis. RESULTS: Immunocytochemistry, combined with electron microscopy, indicated that positive α-actinin expression was like a spheroid particle with high electron density. In addition, the expression was gradually concentrated from the nuclear edge to the cytoplasm and expanded into developing neurites, during differentiation of neural stem cells to neurons. Conversely, energy dispersive X-ray analysis indicated that the more mature the neural differentiation was, and the greater the expression of α-actinin.CONCLUSION: The gradual increase of α-actinin expression is related to growth, development, and maturity of differentiated neuron-like cells, in neonatal rat frontal lobe cortex, at different differentiating time points of NSCs to neurons.
关键词	energy dispersive X-ray analysis alpha-actinin neural stem cells directional differentiation neurons
收录类别	CSCD
语种	英语
资助项目	[National Natural Science Foundation of china] ; [Science and Technology from Educational Committee of Liaoning Provence] ; [Technological Program for Colleges and Universities of Liaoning Educational Committee]
CSCD记录号	CSCD:3635383
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/142111
专题	中国科学院金属研究所
作者单位	中国科学院金属研究所
推荐引用方式 GB/T 7714	Yu Bo,Li Hua,Du Zhe,et al. Alpha-actinin expression at different differentiating time points from temporal lobe cerebral cortex neural stem cells to neuron-like cells using energy dispersive X-ray analysis[J]. NEURAL REGENERATION RESEARCH,2009,4(4):265-270.
APA	Yu Bo,Li Hua,Du Zhe,Hong Yang,Sang Meng,&Shi Yuxiu.(2009).Alpha-actinin expression at different differentiating time points from temporal lobe cerebral cortex neural stem cells to neuron-like cells using energy dispersive X-ray analysis.NEURAL REGENERATION RESEARCH,4(4),265-270.
MLA	Yu Bo,et al."Alpha-actinin expression at different differentiating time points from temporal lobe cerebral cortex neural stem cells to neuron-like cells using energy dispersive X-ray analysis".NEURAL REGENERATION RESEARCH 4.4(2009):265-270.