GlnR positive transcriptional regulation of the phosphate-specific transport system pstSCAB in Amycolatopsis mediterranei U32

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	GlnR positive transcriptional regulation of the phosphate-specific transport system pstSCAB in Amycolatopsis mediterranei U32
其他题名	GlnR positive transcriptional regulation of the phosphate-specific transport system pstSCAB in Amycolatopsis mediterranei U32
	Zhang Yuhui 1; Zhang Yixuan 1; Li Peng 3; Wang Ying 4; Wang Jin 3; Shao Zhihui 3; Zhao Guoping 1
	2018
发表期刊	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
ISSN	1672-9145
卷号	50 期号:8 页码:757-765
摘要	Amycolatopsis mediterranei U32 is an important industrial strain for the production of rifamycin SV. Rifampicin, a derivative of rifamycin SV, is commonly used to treat mycobacterial infections. Although phosphate has long been known to affect rifamycin biosynthesis, phosphate transport, metabolism, and regulation are poorly understood in A. mediterranei. In this study, the functional phosphate transport system pstSCAB was isolated by RNA sequencing and inactivated by insertion mutation in A. mediterranei U32. The mycelium morphology changed from a filamentous shape in the wild-type and pstS1+ strains to irregular swollen shape at the end of filamentous in the Delta pstS1 strain. RT-PCR assay revealed that pstSCAB genes are co-transcribed as a polycistronic messenger. The pstSCAB transcription was significantly activated by nitrate supplementation and positively regulated by GlnR which is a global regulator of nitrogen metabolism in actinomycetes. At the same time, the yield of rifamycin SV decreased after mutation (Delta pstS1) compared with wild-type U32, which indicated a strong connection among phosphate metabolism, nitrogen metabolism, and rifamycin production in actinomycetes.
其他摘要	Amycolatopsis mediterranei U32 is an important industrial strain for the production of rifamycin SV. Rifampicin, a derivative of rifamycin SV, is commonly used to treat mycobacterial infections. Although phosphate has long been known to affect rifamycin biosynthesis, phosphate transport,metabolism, and regulation are poorly understood in A. mediterranei. In this study, the functional phosphate transport system pstSCAB was isolated by RNA sequencing and inactivated by insertion mutation in A. mediterranei U32. The mycelium morphology changed from a filamentous shape in the wild-type and pstS1+ strains to irregular swollen shape at the end of filamentous in the ΔpstS1 strain. RT-PCR assay revealed that pstSCAB genes are co-transcribed as a polycistronic messenger. The pstSCAB transcription was significantly activated by nitrate supplementation and positively regulated by GlnR which is a global regulator of nitrogen metabolism in actinomycetes. At the same time, the yield of rifamycin SV decreased after mutation (ΔpstS1) compared with wild-type U32, which indicated a strong connection among phosphate metabolism, nitrogen metabolism,and rifamycin production in actinomycetes.
关键词	SCANNING-ELECTRON-MICROSCOPY SV PRODUCING ACTINOMYCETE STREPTOMYCES-COELICOLOR ESCHERICHIA-COLI CORYNEBACTERIUM-GLUTAMICUM NITROGEN-METABOLISM BACILLUS-SUBTILIS SIGNAL-TRANSDUCTION BINDING PROTEIN CLONING VECTORS Amycolatopsis mediterranei pstSCAB GlnR phosphate nitrogen
收录类别	CSCD
语种	英语
资助项目	[National Natural Science Foundation of China] ; [Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences]
CSCD记录号	CSCD:6325939
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/142127
专题	中国科学院金属研究所
作者单位	1.中国科学院金属研究所 2.Henan Institute Sci & Technol, Dept Life Sci, Xinxiang 453000, Peoples R China 3.中国科学院 4.天津大学
推荐引用方式 GB/T 7714	Zhang Yuhui,Zhang Yixuan,Li Peng,et al. GlnR positive transcriptional regulation of the phosphate-specific transport system pstSCAB in Amycolatopsis mediterranei U32[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2018,50(8):757-765.
APA	Zhang Yuhui.,Zhang Yixuan.,Li Peng.,Wang Ying.,Wang Jin.,...&Zhao Guoping.(2018).GlnR positive transcriptional regulation of the phosphate-specific transport system pstSCAB in Amycolatopsis mediterranei U32.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,50(8),757-765.
MLA	Zhang Yuhui,et al."GlnR positive transcriptional regulation of the phosphate-specific transport system pstSCAB in Amycolatopsis mediterranei U32".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 50.8(2018):757-765.