| GlnR positive transcriptional regulation of the phosphate-specific transport system pstSCAB in Amycolatopsis mediterranei U32 | |
| Alternative Title | GlnR positive transcriptional regulation of the phosphate-specific transport system pstSCAB in Amycolatopsis mediterranei U32 |
| Zhang Yuhui1; Zhang Yixuan1; Li Peng3; Wang Ying4; Wang Jin3; Shao Zhihui3; Zhao Guoping1 | |
| 2018 | |
| Source Publication | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| ISSN | 1672-9145 |
| Volume | 50Issue:8Pages:757-765 |
| Abstract | Amycolatopsis mediterranei U32 is an important industrial strain for the production of rifamycin SV. Rifampicin, a derivative of rifamycin SV, is commonly used to treat mycobacterial infections. Although phosphate has long been known to affect rifamycin biosynthesis, phosphate transport, metabolism, and regulation are poorly understood in A. mediterranei. In this study, the functional phosphate transport system pstSCAB was isolated by RNA sequencing and inactivated by insertion mutation in A. mediterranei U32. The mycelium morphology changed from a filamentous shape in the wild-type and pstS1+ strains to irregular swollen shape at the end of filamentous in the Delta pstS1 strain. RT-PCR assay revealed that pstSCAB genes are co-transcribed as a polycistronic messenger. The pstSCAB transcription was significantly activated by nitrate supplementation and positively regulated by GlnR which is a global regulator of nitrogen metabolism in actinomycetes. At the same time, the yield of rifamycin SV decreased after mutation (Delta pstS1) compared with wild-type U32, which indicated a strong connection among phosphate metabolism, nitrogen metabolism, and rifamycin production in actinomycetes. |
| Other Abstract | Amycolatopsis mediterranei U32 is an important industrial strain for the production of rifamycin SV. Rifampicin, a derivative of rifamycin SV, is commonly used to treat mycobacterial infections. Although phosphate has long been known to affect rifamycin biosynthesis, phosphate transport,metabolism, and regulation are poorly understood in A. mediterranei. In this study, the functional phosphate transport system pstSCAB was isolated by RNA sequencing and inactivated by insertion mutation in A. mediterranei U32. The mycelium morphology changed from a filamentous shape in the wild-type and pstS1+ strains to irregular swollen shape at the end of filamentous in the ΔpstS1 strain. RT-PCR assay revealed that pstSCAB genes are co-transcribed as a polycistronic messenger. The pstSCAB transcription was significantly activated by nitrate supplementation and positively regulated by GlnR which is a global regulator of nitrogen metabolism in actinomycetes. At the same time, the yield of rifamycin SV decreased after mutation (ΔpstS1) compared with wild-type U32, which indicated a strong connection among phosphate metabolism, nitrogen metabolism,and rifamycin production in actinomycetes. |
| Keyword | SCANNING-ELECTRON-MICROSCOPY SV PRODUCING ACTINOMYCETE STREPTOMYCES-COELICOLOR ESCHERICHIA-COLI CORYNEBACTERIUM-GLUTAMICUM NITROGEN-METABOLISM BACILLUS-SUBTILIS SIGNAL-TRANSDUCTION BINDING PROTEIN CLONING VECTORS Amycolatopsis mediterranei pstSCAB GlnR phosphate nitrogen |
| Indexed By | CSCD |
| Language | 英语 |
| Funding Project | [National Natural Science Foundation of China] ; [Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences] |
| CSCD ID | CSCD:6325939 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.imr.ac.cn/handle/321006/142127 |
| Collection | 中国科学院金属研究所 |
| Affiliation | 1.中国科学院金属研究所 2.Henan Institute Sci & Technol, Dept Life Sci, Xinxiang 453000, Peoples R China 3.中国科学院 4.天津大学 |
| Recommended Citation GB/T 7714 | Zhang Yuhui,Zhang Yixuan,Li Peng,et al. GlnR positive transcriptional regulation of the phosphate-specific transport system pstSCAB in Amycolatopsis mediterranei U32[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2018,50(8):757-765. |
| APA | Zhang Yuhui.,Zhang Yixuan.,Li Peng.,Wang Ying.,Wang Jin.,...&Zhao Guoping.(2018).GlnR positive transcriptional regulation of the phosphate-specific transport system pstSCAB in Amycolatopsis mediterranei U32.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,50(8),757-765. |
| MLA | Zhang Yuhui,et al."GlnR positive transcriptional regulation of the phosphate-specific transport system pstSCAB in Amycolatopsis mediterranei U32".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 50.8(2018):757-765. |
| Files in This Item: | There are no files associated with this item. | |||||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment