Re-expression of methylation-induced tumor suppressor gene silencing is associated with the state of histone modification in gastric cancer cell lines

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	Re-expression of methylation-induced tumor suppressor gene silencing is associated with the state of histone modification in gastric cancer cell lines
其他题名	Re-expression of methylation-induced tumor suppressor gene silencing is associated with the state of histone modification in gastric cancer cell lines
	Meng ChunFeng; Zhu XinJiang; Peng Guo; Dai DongQiu
	2007
发表期刊	WORLD JOURNAL OF GASTROENTEROLOGY
ISSN	1007-9327
卷号	13 期号:46 页码:6166-6171
摘要	AIM: To identify the relationship between DNA hypermethylation and histone modification at a hypermethylated, silenced tumor suppressor gene promoter in human gastric cancer cell lines and to elucidate whether alteration of DNA methylation could affect histone modification.
其他摘要	AIM: To identify the relationship between DNA hypermethylation and histone modification at a hypermethylated, silenced tumor suppressor gene promoter in human gastric cancer cell lines and to elucidate whether alteration of DNA methylation could affect histone modification.METHODS: We used chromatin immunoprecipitation(ChIP) assay to assess the status of histone acetylation and methylation in promoter regions of the p16 and mutL homolog 1(MLH1) genes in 2 gastric cancer cell lines, SGC-7901 and MGC-803. We used methylationspecific PCR (MSP) to evaluate the effect of 5-Aza-2'-deoxycytidine (5-Aza-dC), trichostatin A (TSA) or their combination treatment on DNA methylation status.We used RT-PCR to determine whether alterations of histone modification status after 5-Aza-dC and TSA treatment are reflected in gene expression.RESULTS: For the p16 and MLH1 genes in two cell lines,silenced loci associated with DNA hypermethylation were characterized by histone H3-K9 hypoacetylation and hypermethylation and histone H3-K4 hypomethylation.Treatment with TSA resulted in moderately increased histone H3-K9 acetylation at the silenced loci with no effect on histone H3-K9 methylation and minimal effects on gene expression. In contrast, treatment with 5-Aza-dC rapidly reduced histone H3-K9 methylation at the silenced loci and resulted in reactivation of the two genes. Combined treatment with 5-Aza-dC and TSA was synergistic in reactivating gene expression at the loci showing DNA hypermethylation. Similarly, histone H3-K4 methylation was not affected after TSA treatment, and increased moderately at the silenced loci after 5-Aza-dC treatment.CONCLUSION: Hypermethylation of DNA in promoter CpG islands is related to transcriptional silencing of tumor suppressor genes. Histone H3-K9 methylation in different regions of the promoters studied correlates with DNA methylation status of each gene in gastric cancer cells. However, histone H3-K9 acetylation and H3-K4 methylation inversely correlate with DNA methylation status of each gene in gastric cancer cells. Alteration of DNA methylation affects histone modification.
关键词	MICROSATELLITE INSTABILITY LYSINE-9 METHYLATION DNA METHYLATION H3 LYSINE-9 HYPERMETHYLATION HETEROCHROMATIN PROMOTER EPIGENETICS DEACETYLASE PATTERNS gastric cancer DNA hypermethylation histone methylation histone acetylation p16 mutL homolog 1 5-Aza-2'-deoxycytidine trichostatin A
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:3193869
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/142235
专题	中国科学院金属研究所
作者单位	中国科学院金属研究所
推荐引用方式 GB/T 7714	Meng ChunFeng,Zhu XinJiang,Peng Guo,et al. Re-expression of methylation-induced tumor suppressor gene silencing is associated with the state of histone modification in gastric cancer cell lines[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2007,13(46):6166-6171.
APA	Meng ChunFeng,Zhu XinJiang,Peng Guo,&Dai DongQiu.(2007).Re-expression of methylation-induced tumor suppressor gene silencing is associated with the state of histone modification in gastric cancer cell lines.WORLD JOURNAL OF GASTROENTEROLOGY,13(46),6166-6171.
MLA	Meng ChunFeng,et al."Re-expression of methylation-induced tumor suppressor gene silencing is associated with the state of histone modification in gastric cancer cell lines".WORLD JOURNAL OF GASTROENTEROLOGY 13.46(2007):6166-6171.