Variation of Potential Nitrification and Ammonia-Oxidizing Bacterial Community with Plant-Growing Period in Apple Orchard Soil

IMR OpenIR

	Variation of Potential Nitrification and Ammonia-Oxidizing Bacterial Community with Plant-Growing Period in Apple Orchard Soil
其他题名	Variation of Potential Nitrification and Ammonia-Oxidizing Bacterial Community with Plant-Growing Period in Apple Orchard Soil
	Liu Lingzhi; Qin Sijun; Lu Deguo; Wang Bingying; Yang Zeyuan
	2014
发表期刊	JOURNAL OF INTEGRATIVE AGRICULTURE
ISSN	2095-3119
卷号	13 期号:2 页码:415-425
摘要	In this study, we investigated the potential nitrification and community structure of soil-based ammonia-oxidizing bacteria (AOB) in apple orchard soil during different growth periods and explored the effects of environmental factors on nitrification activity and AOB community composition in the soil of a Hanfu apple orchard, using a culture-dependent technique and denaturing gradient gel electrophoresis (DGGE). We observed that nitrification activity and AOB abundance were the highest in November, lower in May, and the lowest in July. The results of statistical analysis indicated that total nitrogen (N) content, NH4+-N content, NO3--N content, and pH showed significant correlations with AOB abundance and nitrification activity in soil, The Shannon-Winner diversity, as well as species richness and evenness indices (determined by PCR-DGGE banding patterns) in soil samples were the highest in September, but the lowest in July, when compared to additional sampled dates. The DGGE fingerprints of soil-based 16S rRNA genes in November were apparently distinct from those observed in May, July, and September, possessing the lowest species richness indices and the highest dominance indices among all four growth periods. Fourteen DGGE bands were excised for sequencing. The resulting analysis indicated that all AOB communities belonged to the beta-Proteobacteria phylum, with the dominant AOB showing high similarity to the Nitrosospira genus, Therefore, soil-based environmental factors, such as pH variation and content of NH4+-N and NO3--N, can substantially influence the abundance of AOB communities in soil, and play a critical role in soil-based nitrification kinetics.
其他摘要	In this study, we investigated the potential nitriifcation and community structure of soil-based ammonia-oxidizing bacteria (AOB) in apple orchard soil during different growth periods and explored the effects of environmental factors on nitriifcation activity and AOB community composition in the soil of a Hanfu apple orchard, using a culture-dependent technique and denaturing gradient gel electrophoresis (DGGE). We observed that nitrification activity and AOB abundance were the highest in November, lower in May, and the lowest in July. The results of statistical analysis indicated that total nitrogen (N) content, NH4+-N content, NO3--N content, and pH showed signiifcant correlations with AOB abundance and nitriifcation activity in soil. The Shannon-Winner diversity, as well as species richness and evenness indices (determined by PCR-DGGE banding patterns) in soil samples were the highest in September, but the lowest in July, when compared to additional sampled dates. The DGGE ifngerprints of soil-based 16S rRNA genes in November were apparently distinct from those observed in May, July, and September, possessing the lowest species richness indices and the highest dominance indices among all four growth periods. Fourteen DGGE bands were excised for sequencing. The resulting analysis indicated that all AOB communities belonged to the β-Proteobacteriaphylum, with the dominant AOB showing high similarity to theNitrosospira genus. Therefore, soil-based environmental factors, such as pH variation and content of NH4+-N and NO3--N, can substantially inlfuence the abundance of AOB communities in soil, and play a critical role in soil-based nitriifcation kinetics.
关键词	GRADIENT GEL-ELECTROPHORESIS CONIFEROUS FOREST SOILS 16S RIBOSOMAL-RNA NITRIFYING BACTERIA LOW PH PCR DIVERSITY ACID POPULATIONS ARCHAEA apple orchard soil ammonia-oxidizing bacteria potential nitrification community structure PCR-denaturing gradient gel electrophoresis
收录类别	CSCD
语种	英语
资助项目	[National Natural Science Foundation of China] ; [Postdoctoral Science Foundation of China] ; [China Agricultural Research System] ; [Shenyang Municipal Science and Technology Research Projects, China]
CSCD记录号	CSCD:5048474
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/142396
专题	中国科学院金属研究所
作者单位	中国科学院金属研究所
推荐引用方式 GB/T 7714	Liu Lingzhi,Qin Sijun,Lu Deguo,et al. Variation of Potential Nitrification and Ammonia-Oxidizing Bacterial Community with Plant-Growing Period in Apple Orchard Soil[J]. JOURNAL OF INTEGRATIVE AGRICULTURE,2014,13(2):415-425.
APA	Liu Lingzhi,Qin Sijun,Lu Deguo,Wang Bingying,&Yang Zeyuan.(2014).Variation of Potential Nitrification and Ammonia-Oxidizing Bacterial Community with Plant-Growing Period in Apple Orchard Soil.JOURNAL OF INTEGRATIVE AGRICULTURE,13(2),415-425.
MLA	Liu Lingzhi,et al."Variation of Potential Nitrification and Ammonia-Oxidizing Bacterial Community with Plant-Growing Period in Apple Orchard Soil".JOURNAL OF INTEGRATIVE AGRICULTURE 13.2(2014):415-425.