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IbeT, an Escherichia coli K1 Pathogenicity Island Gene, is Essential for Escape From The Lysosomes in Human Brain Microvascular Endothelial Cells
Alternative TitleIbeT, an Escherichia coli K1 Pathogenicity Island Gene, is Essential for Escape From The Lysosomes in Human Brain Microvascular Endothelial Cells
Zhang Ke; Zhao WeiDong; Li Qiang; Fang WenGang; Zhu Li; Chen YuHua
2009
Source PublicationPROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
ISSN1000-3282
Volume36Issue:4Pages:417-423
AbstractEscherichia coli is the most common gram-negative organism causing neonatal meningitis. In order to characterize the role of the pathogenicity island gene ibeT of Escherichia coli K1 in the pathogenesis of neonatal meningitis, an ibeT gene isogenic in-frame deletion mutant strain was constructed. Intracellular survival assay in human brain microvascular endothelial cells (HBMECs) showed that the deletion of ibeT inhibited the growth of Escherichia colt K1 within HBMECs. Confocal microscopy analysis showed that much more ibeT mutant remained in lysosomes of HBMECs compared with wild type Escherichia coli K1 after bacteria invasion into HBMECs. Transmission electron micrographs further showed that ibeT mutant strain was failed to disrupt the Escherichia coli containing vacuole (ECV) and escape into the cytoplasm. Furthermore, cytoplasmic buffering capacities of ibeT mutant were lower than wild type Escherichia coli K1 at pH 6.5 and pH 6.0. These results suggested that the expression of ibeT in Escherichia coli K1 contributed to ECV membrane degradation and subsequent escape from lysosomes into the cytoplasm for replication after Escherichia coli K1 invasion into HBMECs.
KeywordPH TRAFFICKING PENETRATION EXPRESSION PHAGOSOME BARRIER ibeT Escherichia coli K1 HBMEC lysosome escape
Indexed ByCSCD
Language英语
Funding Project[National Natural Science Foundation of China] ; [Doctoral Seeding Fund of Liaoning Province]
CSCD IDCSCD:3616629
Citation statistics
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/143015
Collection中国科学院金属研究所
Affiliation中国科学院金属研究所
Recommended Citation
GB/T 7714
Zhang Ke,Zhao WeiDong,Li Qiang,et al. IbeT, an Escherichia coli K1 Pathogenicity Island Gene, is Essential for Escape From The Lysosomes in Human Brain Microvascular Endothelial Cells[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2009,36(4):417-423.
APA Zhang Ke,Zhao WeiDong,Li Qiang,Fang WenGang,Zhu Li,&Chen YuHua.(2009).IbeT, an Escherichia coli K1 Pathogenicity Island Gene, is Essential for Escape From The Lysosomes in Human Brain Microvascular Endothelial Cells.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,36(4),417-423.
MLA Zhang Ke,et al."IbeT, an Escherichia coli K1 Pathogenicity Island Gene, is Essential for Escape From The Lysosomes in Human Brain Microvascular Endothelial Cells".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 36.4(2009):417-423.
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