Effect of Dy3+ on osteogenic and adipogenic differentiation of mouse primary bone marrow stromal cells and adipocytic trans-differentiation of mouse primary osteoblasts

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	Effect of Dy3+ on osteogenic and adipogenic differentiation of mouse primary bone marrow stromal cells and adipocytic trans-differentiation of mouse primary osteoblasts
其他题名	Effect of Dy~(3+) on osteogenic and adipogenic differentiation of mouse primary bone marrow stromal cells and adipocytic trans-differentiation of mouse primary osteoblasts
	Zhang JinChao 1; Liu DanDan 1; Sun Jing 1; Zhang DaWei 2; Shen ShiGang 1; Yang MengSu 3
	2009
发表期刊	CHINESE SCIENCE BULLETIN
ISSN	1001-6538
卷号	54 期号:1 页码:66-71
摘要	A series of experimental methods including 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) test, alkaline phosphatase ( ALP) activity measurement, mineralized function, Oil Red O stain and measurement were employed to assess the effect of Dy3+ on the osteogenic and adipogenic differentiation of mouse primary bone marrow stromal cells (BMSCs) and the adipogenic trans-differentiation of mouse primary osteoblasts (OBs). The results showed that Dy3+ had no effect on BMSC proliferation at concentrations of 1 x 10(-8) and 1 x 10(-5) mol/L, but inhibited BMSC proliferation at other concentrations. Dy3+ had no effect on OB proliferation at concentrations of 1 x 10(-10) and 1 x 10(-9) mol/L, but inhibited OB proliferation at other concentrations. Dy3+ had no effect on the osteogenic differentiation of BMSCs at concentrations of 1 x 10(-9) and 1 x 10(-7) mol/ L, and promoted osteogenic differentiation of BMSCs at other concentrations at the 7th day. The osteogenic differentiation of BMSCs was inhibited by Dy3+ at concentration of 1 x 10(-5) mol/ L at the 14th day, but promoted osteogenic differentiation of BMSCs at concentrations of 1 x 10(-9), 1 x 10(-8), 1 x 10(-7) and 1 x 10(-6) mol/ L with the maximal effect at concentration of 10(-6) mol/ L. Dy3+ promoted mineralized function of BMSCs at any concentration. Dy3+ had no effect on adipogenic differentiation of BMSCs at concentration of 1 x 10(-7) mol/ L, but inhibited adipogenic differentiation of BMSCs at other concentrations. Dy3+ inhibited adipocytic trans-differentiation of OBs at any concentration, suggesting that Dy3+ had protective effect on bone and the protective effect on bone may be mediated by modulating differentiation of BMSCs away from the adipocyte and inhibiting adipocytic trans-differentiation of OBs which may promote differentiation and mineralization of OBs. These results may be valuable for better understanding the mechanism of the effect of Dy3+ on pathogenesis of osteoporosis.
其他摘要	A series of experimental methods including 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) test, alkaline phosphatase (ALP) activity measurement, mineralized function, Oil Red O stain and measurement were employed to assess the effect of Dy~(3+) on the osteogenic and adipogenic differentiation of mouse primary bone marrow stromal cells (BMSCs) and the adipogenic trans-differentiation of mouse primary osteoblasts (OBs).The results showed that Dy~(3+) had no effect on BMSC proliferation at concentrations of 1×10~(-8) and 1×10~(-5) mol/L,but inhibited BMSC proliferation at other concentrations.Dy~(3+) had no effect on OB proliferation at concentrations of 1×10~(-10) and 1×10~(-9) mol/L,but inhibited OB proliferation at other concentrations.Dy~(3+) had no effect on the osteogenic differentiation of BMSCs at concentrations of 1×10~(-9) and 1×10~(-7) mol/L,and promoted osteogenic differentiation of BMSCs at other concentrations at the 7th day.The osteogenic differentiation of BMSCs was inhibited by Dy~(3+) at concentration of 1×10~(-5) mol/L at the 14th day,but promoted osteogenic differentiation of BMSCs at concentrations of 1×10~(-9),1×10~(-8),1×10~(-7) and 1×10~(-6) mol/L with the maximal effect at concentration of 10~(-6) mol/L.Dy~(3+) promoted mineralized function of BMSCs at any concentration.Dy~(3+) had no effect on adipogenic differentiation of BMSCs at concentration of 1×10~(-7) mol/L,but inhibited adipogenic differentiation of BMSCs at other concentrations.Dy~(3+) inhibited adipocytic trans-differentiation of OBs at any concentration,suggesting that Dy~(3+) had protective effect on bone and the protective effect on bone may be mediated by modulating differentiation of BMSCs away from the adipocyte and inhibiting adipocytic trans-differentiation of OBs which may promote differentiation and mineralization of OBs.These results may be valuable for better understanding the mechanism of the effect of Dy~(3+) on pathogenesis of osteoporosis.
关键词	OSTEOCLAST-LIKE CELLS IN-VITRO SUPPORT DIFFERENTIATION PROLIFERATION OSTEOPOROSIS RESORPTION CULTURES PROTEIN GROWTH IONS rare earth ion bone marrow stromal cells osteogenic differentiation adipogenic differentiation adipocytic trans-differentiation
收录类别	CSCD
语种	英语
资助项目	[Ministry of Education of China]
CSCD记录号	CSCD:3565991
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/143143
专题	中国科学院金属研究所
作者单位	1.河北大学 2.中国科学院金属研究所 3.City University Hong Kong, Dept Biol & Chem, Hong Kong, Hong Kong, Peoples R China
推荐引用方式 GB/T 7714	Zhang JinChao,Liu DanDan,Sun Jing,et al. Effect of Dy3+ on osteogenic and adipogenic differentiation of mouse primary bone marrow stromal cells and adipocytic trans-differentiation of mouse primary osteoblasts[J]. CHINESE SCIENCE BULLETIN,2009,54(1):66-71.
APA	Zhang JinChao,Liu DanDan,Sun Jing,Zhang DaWei,Shen ShiGang,&Yang MengSu.(2009).Effect of Dy3+ on osteogenic and adipogenic differentiation of mouse primary bone marrow stromal cells and adipocytic trans-differentiation of mouse primary osteoblasts.CHINESE SCIENCE BULLETIN,54(1),66-71.
MLA	Zhang JinChao,et al."Effect of Dy3+ on osteogenic and adipogenic differentiation of mouse primary bone marrow stromal cells and adipocytic trans-differentiation of mouse primary osteoblasts".CHINESE SCIENCE BULLETIN 54.1(2009):66-71.