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Intestinal microflora molecular markers of spleen-deficient rats and evaluation of traditional Chinese drugs
Alternative TitleIntestinal microflora molecular markers of spleen-deficient rats and evaluation of traditional Chinese drugs
Peng Ying1; Wang Zhuo1; Lu Yuan3; Wu ChunFu2; Yang JingYu2; Li XiaoBo1
2009
Source PublicationWORLD JOURNAL OF GASTROENTEROLOGY
ISSN1007-9327
Volume15Issue:18Pages:2220-2227
AbstractAIM: To find a rapid and efficient analysis method of gastrointestinal microflora in Pi-deficient (spleen-deficient) rats and to evaluate traditional Chinese drugs.
Other AbstractAIM: To find a rapid and efficient analysis method of gastrointestinal microflora in Pi-deficient (spleendeficient) rats and to evaluate traditional Chinese drugs. METHODS: Enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) based assay was performed to examine changes of intestinal microflora in two Pi-deficienct animal models and to evaluate the efficacy of four traditional Chinese drugs as well as a probiotic recipe and another therapy in Pi-deficient rats. RESULTS: A molecular marker was identified for Pideficiency in rats. The pharmacodynamic evaluation system, including identified molecular markers (net integral area and abundance of DNA bands), Shannon's index for diversity of intestinal microflora, and Sorenson's pairwise similarity coefficient, was established. The four major clinical recipes of traditional Chinese drugs for Pi-deficiency in rats, especially at their medium dose (equivalence to the clinical dose), produced more pronounced recovery activities in Pi-deficient rats, while higher doses of these recipes did not show a better therapeutic effect but some toxic effects such as perturbation deterioration of intestinal microflora. CONCLUSION: Both fingerprint analysis and identified marker can show Pi-deficiency in rats and its difference after treatment. The identified molecular marker may be applied in screening for the active compounds both in relative traditional Chinese drugs and in pharmacodynamic study of Pi-deficiency in rats.
KeywordERIC-PCR MICROBIOTA Pi-deficiency Enterobacterial repetitive intergenic consensus-PCR Traditional Chinese medicine
Indexed ByCSCD
Language英语
Funding Project[National Natural Science Foundation of China]
CSCD IDCSCD:3614421
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Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/143180
Collection中国科学院金属研究所
Affiliation1.上海交通大学
2.中国科学院金属研究所
3.Newcastle Univ, No Institute Canc Res, Newcastle Upon Tyne NE2 4AA, Tyne & Wear, England
Recommended Citation
GB/T 7714
Peng Ying,Wang Zhuo,Lu Yuan,et al. Intestinal microflora molecular markers of spleen-deficient rats and evaluation of traditional Chinese drugs[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2009,15(18):2220-2227.
APA Peng Ying,Wang Zhuo,Lu Yuan,Wu ChunFu,Yang JingYu,&Li XiaoBo.(2009).Intestinal microflora molecular markers of spleen-deficient rats and evaluation of traditional Chinese drugs.WORLD JOURNAL OF GASTROENTEROLOGY,15(18),2220-2227.
MLA Peng Ying,et al."Intestinal microflora molecular markers of spleen-deficient rats and evaluation of traditional Chinese drugs".WORLD JOURNAL OF GASTROENTEROLOGY 15.18(2009):2220-2227.
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