Intestinal microflora molecular markers of spleen-deficient rats and evaluation of traditional Chinese drugs

IMR OpenIR

	Intestinal microflora molecular markers of spleen-deficient rats and evaluation of traditional Chinese drugs
其他题名	Intestinal microflora molecular markers of spleen-deficient rats and evaluation of traditional Chinese drugs
	Peng Ying 1; Wang Zhuo 1; Lu Yuan 3; Wu ChunFu 2; Yang JingYu 2; Li XiaoBo 1
	2009
发表期刊	WORLD JOURNAL OF GASTROENTEROLOGY
ISSN	1007-9327
卷号	15 期号:18 页码:2220-2227
摘要	AIM: To find a rapid and efficient analysis method of gastrointestinal microflora in Pi-deficient (spleen-deficient) rats and to evaluate traditional Chinese drugs.
其他摘要	AIM: To find a rapid and efficient analysis method of gastrointestinal microflora in Pi-deficient (spleendeficient) rats and to evaluate traditional Chinese drugs. METHODS: Enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) based assay was performed to examine changes of intestinal microflora in two Pi-deficienct animal models and to evaluate the efficacy of four traditional Chinese drugs as well as a probiotic recipe and another therapy in Pi-deficient rats. RESULTS: A molecular marker was identified for Pideficiency in rats. The pharmacodynamic evaluation system, including identified molecular markers (net integral area and abundance of DNA bands), Shannon's index for diversity of intestinal microflora, and Sorenson's pairwise similarity coefficient, was established. The four major clinical recipes of traditional Chinese drugs for Pi-deficiency in rats, especially at their medium dose (equivalence to the clinical dose), produced more pronounced recovery activities in Pi-deficient rats, while higher doses of these recipes did not show a better therapeutic effect but some toxic effects such as perturbation deterioration of intestinal microflora. CONCLUSION: Both fingerprint analysis and identified marker can show Pi-deficiency in rats and its difference after treatment. The identified molecular marker may be applied in screening for the active compounds both in relative traditional Chinese drugs and in pharmacodynamic study of Pi-deficiency in rats.
关键词	ERIC-PCR MICROBIOTA Pi-deficiency Enterobacterial repetitive intergenic consensus-PCR Traditional Chinese medicine
收录类别	CSCD
语种	英语
资助项目	[National Natural Science Foundation of China]
CSCD记录号	CSCD:3614421
引用统计	被引频次：1[CSCD] [CSCD记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/143183
专题	中国科学院金属研究所
作者单位	1.上海交通大学 2.中国科学院金属研究所 3.Newcastle Univ, No Institute Canc Res, Newcastle Upon Tyne NE2 4AA, Tyne & Wear, England
推荐引用方式 GB/T 7714	Peng Ying,Wang Zhuo,Lu Yuan,et al. Intestinal microflora molecular markers of spleen-deficient rats and evaluation of traditional Chinese drugs[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2009,15(18):2220-2227.
APA	Peng Ying,Wang Zhuo,Lu Yuan,Wu ChunFu,Yang JingYu,&Li XiaoBo.(2009).Intestinal microflora molecular markers of spleen-deficient rats and evaluation of traditional Chinese drugs.WORLD JOURNAL OF GASTROENTEROLOGY,15(18),2220-2227.
MLA	Peng Ying,et al."Intestinal microflora molecular markers of spleen-deficient rats and evaluation of traditional Chinese drugs".WORLD JOURNAL OF GASTROENTEROLOGY 15.18(2009):2220-2227.