| Application of phage-displayed single chain antibodies in western blot | |
| Alternative Title | Application of Phage-displayed Single Chain Antibodies in Western Blot |
| Ding YL1; Liu MY1; Han W1; Yang SL1; Liu H1; Gong Y1 | |
| 2005 | |
| Source Publication | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| ISSN | 1672-9145 |
| Volume | 37Issue:3Pages:205-209 |
| Abstract | A phage display single chain fragment variable library constructed on pIII protein of M13 filamentous phage was screened using B-lymphocyte stimulator and FP248 as selective molecules. After four rounds of panning, there was a remarkable enrichment in the titer of bound phages. Twenty phage clones were selected from the last round and screened by means of phage-ELISA. With the antibody phages as primary antibodies in Western blot, we developed a method for detecting the specific antigen. The dilutions of antibody phages depend on the affinity between antibody-displayed phage particles and antigens. |
| Other Abstract | A phage display single chain fragment variable library constructed on pIII protein of M13 filamentous phage was screened using B-lymphocyte stimulator and FP248 as selective molecules. After four rounds of panning, there was a remarkable enrichment in the titer of bound phages. Twenty phage clones were selected from the last round and screened by means of phage-ELISA. With the antibody phages as primary antibodies in Western blot, we developed a method for detecting the specific antigen. The dilutions of antibody phages depend on the affinity between antibody-displayed phage particles and antigens. |
| Keyword | B-LYMPHOCYTE STIMULATOR FUSION PHAGE TUMOR-CELLS LIBRARY ANTIGENS PROTEIN IDENTIFICATION SELECTION PEPTIDE BLYS phage-display anitbody Western blot |
| Indexed By | CSCD |
| Language | 英语 |
| CSCD ID | CSCD:1958580 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.imr.ac.cn/handle/321006/143791 |
| Collection | 中国科学院金属研究所 |
| Affiliation | 1.中国科学院 2.中国科学院金属研究所 |
| Recommended Citation GB/T 7714 | Ding YL,Liu MY,Han W,et al. Application of phage-displayed single chain antibodies in western blot[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2005,37(3):205-209. |
| APA | Ding YL,Liu MY,Han W,Yang SL,Liu H,&Gong Y.(2005).Application of phage-displayed single chain antibodies in western blot.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,37(3),205-209. |
| MLA | Ding YL,et al."Application of phage-displayed single chain antibodies in western blot".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 37.3(2005):205-209. |
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