IMR OpenIR
Application of phage-displayed single chain antibodies in western blot
Alternative TitleApplication of Phage-displayed Single Chain Antibodies in Western Blot
Ding YL1; Liu MY1; Han W1; Yang SL1; Liu H1; Gong Y1
2005
Source PublicationACTA BIOCHIMICA ET BIOPHYSICA SINICA
ISSN1672-9145
Volume37Issue:3Pages:205-209
AbstractA phage display single chain fragment variable library constructed on pIII protein of M13 filamentous phage was screened using B-lymphocyte stimulator and FP248 as selective molecules. After four rounds of panning, there was a remarkable enrichment in the titer of bound phages. Twenty phage clones were selected from the last round and screened by means of phage-ELISA. With the antibody phages as primary antibodies in Western blot, we developed a method for detecting the specific antigen. The dilutions of antibody phages depend on the affinity between antibody-displayed phage particles and antigens.
Other AbstractA phage display single chain fragment variable library constructed on pIII protein of M13 filamentous phage was screened using B-lymphocyte stimulator and FP248 as selective molecules. After four rounds of panning, there was a remarkable enrichment in the titer of bound phages. Twenty phage clones were selected from the last round and screened by means of phage-ELISA. With the antibody phages as primary antibodies in Western blot, we developed a method for detecting the specific antigen. The dilutions of antibody phages depend on the affinity between antibody-displayed phage particles and antigens.
KeywordB-LYMPHOCYTE STIMULATOR FUSION PHAGE TUMOR-CELLS LIBRARY ANTIGENS PROTEIN IDENTIFICATION SELECTION PEPTIDE BLYS phage-display anitbody Western blot
Indexed ByCSCD
Language英语
CSCD IDCSCD:1958580
Citation statistics
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/143791
Collection中国科学院金属研究所
Affiliation1.中国科学院
2.中国科学院金属研究所
Recommended Citation
GB/T 7714
Ding YL,Liu MY,Han W,et al. Application of phage-displayed single chain antibodies in western blot[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2005,37(3):205-209.
APA Ding YL,Liu MY,Han W,Yang SL,Liu H,&Gong Y.(2005).Application of phage-displayed single chain antibodies in western blot.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,37(3),205-209.
MLA Ding YL,et al."Application of phage-displayed single chain antibodies in western blot".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 37.3(2005):205-209.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Ding YL]'s Articles
[Liu MY]'s Articles
[Han W]'s Articles
Baidu academic
Similar articles in Baidu academic
[Ding YL]'s Articles
[Liu MY]'s Articles
[Han W]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Ding YL]'s Articles
[Liu MY]'s Articles
[Han W]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.