Application of phage-displayed single chain antibodies in western blot

IMR OpenIR

	Application of phage-displayed single chain antibodies in western blot
其他题名	Application of Phage-displayed Single Chain Antibodies in Western Blot
	Ding YL 1; Liu MY 1; Han W 1; Yang SL 1; Liu H 1; Gong Y 1
	2005
发表期刊	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
ISSN	1672-9145
卷号	37 期号:3 页码:205-209
摘要	A phage display single chain fragment variable library constructed on pIII protein of M13 filamentous phage was screened using B-lymphocyte stimulator and FP248 as selective molecules. After four rounds of panning, there was a remarkable enrichment in the titer of bound phages. Twenty phage clones were selected from the last round and screened by means of phage-ELISA. With the antibody phages as primary antibodies in Western blot, we developed a method for detecting the specific antigen. The dilutions of antibody phages depend on the affinity between antibody-displayed phage particles and antigens.
其他摘要	A phage display single chain fragment variable library constructed on pIII protein of M13 filamentous phage was screened using B-lymphocyte stimulator and FP248 as selective molecules. After four rounds of panning, there was a remarkable enrichment in the titer of bound phages. Twenty phage clones were selected from the last round and screened by means of phage-ELISA. With the antibody phages as primary antibodies in Western blot, we developed a method for detecting the specific antigen. The dilutions of antibody phages depend on the affinity between antibody-displayed phage particles and antigens.
关键词	B-LYMPHOCYTE STIMULATOR FUSION PHAGE TUMOR-CELLS LIBRARY ANTIGENS PROTEIN IDENTIFICATION SELECTION PEPTIDE BLYS phage-display anitbody Western blot
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:1958580
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/143791
专题	中国科学院金属研究所
作者单位	1.中国科学院 2.中国科学院金属研究所
推荐引用方式 GB/T 7714	Ding YL,Liu MY,Han W,et al. Application of phage-displayed single chain antibodies in western blot[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2005,37(3):205-209.
APA	Ding YL,Liu MY,Han W,Yang SL,Liu H,&Gong Y.(2005).Application of phage-displayed single chain antibodies in western blot.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,37(3),205-209.
MLA	Ding YL,et al."Application of phage-displayed single chain antibodies in western blot".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 37.3(2005):205-209.