| Echinacoside induces rat pulmonary artery vasorelaxation by opening the NO-cGMP-PKG-BKCa channels and reducing intracellular Ca2+ levels | |
| Alternative Title | Echinacoside induces rat pulmonary artery vasorelaxation by opening the NO-cGMP-PKG-BK Ca channels and reducing intracellular Ca 2+ levels |
| Gai Xiangyun1; Wei Yuhai3; Zhang Wei2; Wuren Tana2; Wang Yaping2; Li Zhanqiang2; Liu Shou2; Ma Lan2; Lu Dianxiang2; Zhou Yi1; Ge Rili1 | |
| 2015 | |
| Source Publication | ACTA PHARMACOLOGICA SINICA
 |
| ISSN | 1671-4083 |
| Volume | 36Issue:5Pages:587-596 |
| Abstract | Aim: Sustained pulmonary vasoconstriction as experienced at high altitude can lead to pulmonary hypertension (PH). The main purpose of this study is to investigate the vasorelaxant effect of echinacoside (ECH), a phenylethanoid glycoside from the Tibetan herb Lagotis brevituba Maxim and Cistanche tubulosa, on the pulmonary artery and its potential mechanism. |
| Other Abstract | Aim: Sustained pulmonary vasoconstriction as experienced at high altitude can lead to pulmonary hypertension (PH). The main purpose of this study is to investigate the vasorelaxant effect of echinacoside (ECH), a phenylethanoid glycoside from the Tibetan herb Lagotis brevituba Maxim and Cistanche tubulosa , on the pulmonary artery and its potential mechanism. Methods: Pulmonary arterial rings obtained from male Wistar rats were suspended in organ chambers filled with Krebs-Henseleit solution, and isometric tension was measured using a force transducer. Intracellular Ca 2+ levels were measured in cultured rat pulmonary arterial smooth muscle cells (PASMCs) using Fluo 4-AM. Results: ECH (30–300 μmol 50 in endothelium-denuded rings. This effect was significantly blocked by L-NAME, TEA, and BaCl 2 . However, IMT, 4-AP, and Gli did not inhibit ECH-induced relaxation. Under extracellular Ca 2+ -free conditions, the maximum contraction was reduced to 24.54%±2.97% and 10.60%±2.07% in rings treated with 100 and 300 μmol Conclusion: ECH suppresses NE-induced contraction of rat pulmonary artery via reducing intracellular Ca 2+ levels, and induces its relaxation through the NO-cGMP pathway and opening of K + channels (BK Ca and K IR ). |
| Keyword | ACTIVATED POTASSIUM CHANNELS K+ CHANNELS CALCIUM-CHANNEL Tibetan herb echinacoside pulmonary hypertension high altitude vasorelaxation NO BKCa K-IR artery ring pulmonary arterial smooth muscle cells |
| Indexed By | CSCD |
| Language | 英语 |
| Funding Project | [National Program on Key Basic Research Project of China] ; [Program of International Science and Technology Cooperation of China] ; [National Natural Science Foundation of China] ; [Key Laboratories Development Program of Qinghai Province] ; [Qinghai-Utah Joint Research Key Lab for High Altitude Medicine] ; [Ministry of Education New Century Outstanding Talents Support Program of China] ; [Natural Science Foundation of Qinghai] |
| CSCD ID | CSCD:5425432 |
| Citation statistics |
Cited Times:2[CSCD]
[CSCD Record]
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| Document Type | 期刊论文 |
| Identifier | http://ir.imr.ac.cn/handle/321006/143845 |
| Collection | 中国科学院金属研究所 |
| Affiliation | 1.中国科学院金属研究所 2.青海大学 3.Qinghai Entry Exit Inspect & Quarantine Bur, Xining 810001, Peoples R China |
| Recommended Citation GB/T 7714 | Gai Xiangyun,Wei Yuhai,Zhang Wei,et al. Echinacoside induces rat pulmonary artery vasorelaxation by opening the NO-cGMP-PKG-BKCa channels and reducing intracellular Ca2+ levels[J]. ACTA PHARMACOLOGICA SINICA,2015,36(5):587-596. |
| APA | Gai Xiangyun.,Wei Yuhai.,Zhang Wei.,Wuren Tana.,Wang Yaping.,...&Ge Rili.(2015).Echinacoside induces rat pulmonary artery vasorelaxation by opening the NO-cGMP-PKG-BKCa channels and reducing intracellular Ca2+ levels.ACTA PHARMACOLOGICA SINICA,36(5),587-596. |
| MLA | Gai Xiangyun,et al."Echinacoside induces rat pulmonary artery vasorelaxation by opening the NO-cGMP-PKG-BKCa channels and reducing intracellular Ca2+ levels".ACTA PHARMACOLOGICA SINICA 36.5(2015):587-596. |
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