体外诱导成骨细胞在降解性镁合金表面的附着与增殖

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	体外诱导成骨细胞在降解性镁合金表面的附着与增殖
其他题名	Attachment and proliferation of induced osteoblasts from bone marrow stromal stem cells in vitro on degradable magnesium alloy
	潘锋 1; 柏树令 1; 张二林 2; 于国宁 3; 徐丽萍 2
	2010
发表期刊	解剖学报
ISSN	0529-1356
卷号	000 期号:003 页码:430-434
摘要	目的观察诱导后的成骨细胞在Mg-Mn-Zn表面附着与增殖情况. 方法分离培养兔骨髓基质干细胞（BMSCs）,将其诱导分化为成骨细胞后,以1×108/L的细胞浓度种植在Mg-Mn-Zn及对照组纯钛表面,联合培养1、3、5d后,分别用扫描电镜、激光扫描共焦显微镜观察两种金属表面的细胞形态学变化及生长情况.结果 BMSCs以球状体方式进行克隆生长,经诱导后的细胞表达成骨细胞特性,在与镁合金复合培养后, 细胞发生黏附并增殖连接成片. 结论诱导后的成骨细胞在Mg-Mn-Zn表面有较好的分裂增殖能力,提示镁合金有较好的骨诱导生成作用.
其他摘要	Objective To investigate the attachment and proliferation of osteoblasts from bone marrow stromal stem ceils （BMSCs） on the surface of Mg-Mn-Zn alloy. Methods Rabbit BMSCs were isolated by density gradient centrifugation method and amplified in the flasks, using the osteogenic inducing conditions （OGC） as the culture media. The osteogenic potential of BMSCs was investigated by means of Alizarin red staining, alkaline phosphatase （ALP） staining, collogen I immunohistochemisty technology , scanning electron microscope （SEM） and transmission electron microscope（TEM）. Besides, induced osteoblasts were seeded on the surface of Mg-Mn-Zn alloy and pure Ti by 1× 10^5/ ml. Scanning electron microscopy and laser scanning confocal microscopy were carried out to observe cell morphology and growth at 1, 3 and 5 days under co-culture condition. Results Rabbit BMSCs growed in a great number and in the form of cell spheroids and the cells outgrew in all directions firstly. These cells were then induced into osteoblasts which could adhere to the surface of Mg-Mn-Zn and connected with each other to form lamellar substances. Conclusion Osteoblasts from BMSCs have good adhesion and growth capability on the surface of Mg-Mn-Zn indicating that magnesium alloy posses good bone induction function.
关键词	Mg-Mn-Zn合金骨髓基质干细胞钛扫描电镜激光扫描共焦显微镜兔
收录类别	CSCD
语种	中文
CSCD记录号	CSCD:3940567
引用统计	被引频次：1[CSCD] [CSCD记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/145132
专题	中国科学院金属研究所
作者单位	1.中国医科大学 2.中国科学院金属研究所 3.辽宁省人民医院
推荐引用方式 GB/T 7714	潘锋,柏树令,张二林,等. 体外诱导成骨细胞在降解性镁合金表面的附着与增殖[J]. 解剖学报,2010,000(003):430-434.
APA	潘锋,柏树令,张二林,于国宁,&徐丽萍.(2010).体外诱导成骨细胞在降解性镁合金表面的附着与增殖.解剖学报,000(003),430-434.
MLA	潘锋,et al."体外诱导成骨细胞在降解性镁合金表面的附着与增殖".解剖学报 000.003(2010):430-434.