Expression of recombinant tryptophan decarboxylase in different subcellular compartments in tobacco plant

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	Expression of recombinant tryptophan decarboxylase in different subcellular compartments in tobacco plant
其他题名	Expression of recombinant tryptophan decarboxylase in different subcellular compartments in tobacco plant
	Wang M 1; Li QR 1; Di Fiore S 1; Fischer R 1
	2002
发表期刊	ACTA BOTANICA SINICA
ISSN	0577-7496
卷号	44 期号:3 页码:314-317
摘要	The gene encoded for tryptophan decarboxylase (TDC), which is the key enzyme in terpenoil indole alkaloids pathway, was targeted to different subcellular compartments and stably expressed in transgenic tobacco (Nicotiana tabacum L.) plants at the levels detected by Western blot and tryptamine accumulation analysis. It was shown that the TDC was located in subcellular compartments, the chloroplasts and cytosol. The recombinant TDC targeted to chloroplasts and cytosol in tobacco plants was effectively expressed as soluble protein by Western blot analysis and enzymatic assay. The level of tryptamine accumulation in chloroplast was higher than that in cytosol and very low in vacuole and endoplasmic reticulum (ER) to be hardly detected by Western blot analysis. It was indicated that the highest amount of tryptamine was in chloroplasts, lower in endoplasmic reticula and the lowest in vacuoles as compared to those in wild type plants. The TDC targeted to different subcellular compartments of tobacco plants and its expression level were studied by different nucleotide sequences coding signal peptides at 5'-end of tdc gene in order to know the effects of the TDC in compartmentation on its functionality.
其他摘要	The gene encoded for tryptophan decarboxylase (TDC), which is the key enzyme in terpenoil indole alkaloids pathway, was targeted to different subcellular compartments and stably expressed in transgenic tobacco (Nicotiana tabacum L.) plants at the levels detected by Western blot and tryptamine accumulation analysis. It was shown that the TDC was located in subcellular compartments, the chloroplasts and cytosol. The recombinant TDC targeted to chloroplasts and cytosol in tobacco plants was effectively expressed as soluble protein by Western blot analysis and enzymatic assay. The level of tryptamine accumulation in chloroplast was higher than that in cytosol and very low in vacuole and endoplasmic reticulum (ER) to be hardly detected by Western blot analysis. It was indicated that the highest amount of tryptamine was in chloroplasts, lower in endoplasmic reticula and the lowest in vacuoles as compared to those in wild type plants. The TDC targeted to different subcellular compartments of tobacco plants and its expression level were studied by different nucleotide sequences coding signal peptides at 5'-end of tdc gene in order to know the effects of the TDC in compartmentation on its functionality.
关键词	CATHARANTHUS-ROSEUS ALKALOIDS PROTEINS ER tryptophan decarboxylase alkaloids biosynthetic pathway Nicotiana tabacum subcellular compartment
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:1365022
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/145597
专题	中国科学院金属研究所
作者单位	1.中国科学院 2.中国科学院金属研究所 3.Rhein Westfal TH Aachen
推荐引用方式 GB/T 7714	Wang M,Li QR,Di Fiore S,et al. Expression of recombinant tryptophan decarboxylase in different subcellular compartments in tobacco plant[J]. ACTA BOTANICA SINICA,2002,44(3):314-317.
APA	Wang M,Li QR,Di Fiore S,&Fischer R.(2002).Expression of recombinant tryptophan decarboxylase in different subcellular compartments in tobacco plant.ACTA BOTANICA SINICA,44(3),314-317.
MLA	Wang M,et al."Expression of recombinant tryptophan decarboxylase in different subcellular compartments in tobacco plant".ACTA BOTANICA SINICA 44.3(2002):314-317.