LC-MS/MS Method for the Quantitation of Cefotetan in Human Plasma and Its Application to Pharmacokinetic Study

IMR OpenIR

	LC-MS/MS Method for the Quantitation of Cefotetan in Human Plasma and Its Application to Pharmacokinetic Study
其他题名	LC-MS/MS Method for the Quantitation of Cefotetan in Human Plasma and Its Application to Pharmacokinetic Study
	Shi Meiyun 1; Yin Lei 1; Cai Lanlan 1; Wang Can 1; Liu Xidong 1; Zhao Sen 1; Sun Yantong 1; Fawcett J Paul 3; Zhao Limei 4; Yang Yan 1; Gu Jingkai 1
	2014
发表期刊	CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
ISSN	1005-9040
卷号	30 期号:6 页码:900-904
摘要	A rapid and sensitive liquid chromatography-tandem mass spectrometry(LC-MS/MS) method for the determination of cefotetan in human plasma was developed and validated. After the protein precipitation of sample with acetonitrile, the analyte and internal standard(IS), tramadol, were separated on a Zorbax XDB C-8 column using acetonitrile/1%(volume fraction) formic acid(volume ratio 35:65, pH=2.5) as mobile phase at a flow rate of 1.0 mL/min with a 1:1 split. The detection was performed by electrospray ionization with positive ion mode, followed by multiple reaction monitoring of the transitions for cefotetan at m/z 576.3 -> 460.2(quantifier) and m/z 576.3 -> 432.2(qualifier) and for IS at m/z 264.1 -> 58.1. Cefotetan and IS were eluted at 1.86 and 1.87 min, respectively. The assay was linear over the concentration range of 0.1 -> 100 mu g/mL for 20 mu L of human plasma only with intra-and inter-day precisions(expressed as the relative standard deviation) of less than 6.62% and accuracies(as relative error) of +/- 1.31%. The method was applied to the pharmacokinetic study of a 1-h intravenous infusion of 1.0 g of cefotetan disodium for human volunteers(n=6).
其他摘要	A rapid and sensitive liquid chromatography-tandem mass spectrometry(LC-MS/MS) method for the determination of cefotetan in human plasma was developed and validated. After the protein precipitation of sample with acetonitrile, the analyte and internal standard(IS), tramadol, were separated on a Zorbax XDB C_8 column using acetonitrile/1%(volume fraction) formic acid(volume ratio 35:65, pH=2.5) as mobile phase at a flow rate of 1.0 mL/min with a 1:1 split. The detection was performed by electrospray ionization with positive ion mode, followed by multiple reaction monitoring of the transitions for cefotetan at m/z 576.3→460.2(quantifier) and m/z 576.3→432.2(qualifier) and for IS at m/z 264.1→58.1. Cefotetan and IS were eluted at 1.86 and 1.87 min, respectively. The assay was linear over the concentration range of 0.1―100 μg/mL for 20 μL of human plasma only with intra- and inter-day precisions(expressed as the relative standard deviation) of less than 6.62% and accuracies(as relative error) of ±1.31%. The method was applied to the pharmacokinetic study of a 1-h intravenous infusion of 1.0 g of cefotetan disodium for human volunteers(n=6).
关键词	HEALTHY CHINESE VOLUNTEERS DISODIUM SINGLE INJECTION Cefotetan Liquid chromatography-tandem mass spectrometry(LC-MS/MS) Pharmacokinetics Human plasma
收录类别	CSCD
语种	英语
资助项目	[National Natural Science Foundation of China] ; [Preject of China Equipment and Education Resources System(CERS)]
CSCD记录号	CSCD:5301737
引用统计	被引频次：2[CSCD] [CSCD记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/145676
专题	中国科学院金属研究所
作者单位	1.吉林大学 2.中山大学 3.University Otago, Sch Pharm, Dunedin 9054, New Zealand 4.中国科学院金属研究所
推荐引用方式 GB/T 7714	Shi Meiyun,Yin Lei,Cai Lanlan,et al. LC-MS/MS Method for the Quantitation of Cefotetan in Human Plasma and Its Application to Pharmacokinetic Study[J]. CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,2014,30(6):900-904.
APA	Shi Meiyun.,Yin Lei.,Cai Lanlan.,Wang Can.,Liu Xidong.,...&Gu Jingkai.(2014).LC-MS/MS Method for the Quantitation of Cefotetan in Human Plasma and Its Application to Pharmacokinetic Study.CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,30(6),900-904.
MLA	Shi Meiyun,et al."LC-MS/MS Method for the Quantitation of Cefotetan in Human Plasma and Its Application to Pharmacokinetic Study".CHEMICAL RESEARCH IN CHINESE UNIVERSITIES 30.6(2014):900-904.