Ligustrazine facilitates hair cell regeneration in the cochlea following gentamicin ototoxicity

IMR OpenIR

	Ligustrazine facilitates hair cell regeneration in the cochlea following gentamicin ototoxicity
其他题名	Ligustrazine facilitates hair cell regeneration in the cochlea following gentamicin ototoxicity
	Zhang Liang; Ni Yueqiu; Li Yufang; Fu Wenshuang
	2010
发表期刊	NEURAL REGENERATION RESEARCH
ISSN	1673-5374
卷号	5 期号:10 页码:735-740
摘要	BACKGROUND: Ligustrazine (tetramethylpyrazine) decreases ototoxicity induced by gentamicin and facilitates hair cell regeneration and repair, but the precise mechanisms remain controversial
其他摘要	BACKGROUND: Ligustrazine (tetramethylpyrazine) decreases ototoxicity induced by gentamicin and facilitates hair cell regeneration and repair, but the precise mechanisms remain controversial. OBJECTIVE: To explore the protective effects of ligustrazine on gentamicin ototoxicity by determining heat shock protein 70 mRNA and protein expression in the cochlear stria vascularis of guinea pigs at different time points. DESIGN, TIME AND SETTING: The randomized, controlled study was performed at the Laboratory of Physiology, Shenyang Medical College of China in 2007. MATERIALS: Ligustrazine parenteral solution (Qiqihar Pharmaceutical Factory, China) and gentamicin sulfate (Shenyang First Pharmaceutical Factory, China) were used in this experiment. METHODS: White guinea pigs with red eyes were randomly intraperitoneally administered gentamicin sulfate injection + saline, gentamicin sulfate injection + ligustrazine, and ligustrazine + saline, respectively. MAIN OUTCOME MEASURES: Auditory brainstem response threshold was measured. Immunohistochemistry, in situ hybridization, and image analyzing techniques were utilized to determine heat shock protein 70 mRNA and protein expression in cochlear stria vascularis of guinea pigs. RESULTS: Following gentamicin ototoxicity, the auditory brainstem response threshold increased, peaked on day 3, and then decreased with increased time after drug withdrawal. The auditory brainstem response threshold was significantly diminished following ligustrazine intervention, but recovered to normal on day 30 (P < 0.05). Heat shock protein 70 expression also increased, peaked on day 3, and then decreased in the cochlear stria vascularis of guinea pigs following gentamicin ototoxicity. Ligustrazine intervention resulted in decreased heat shock protein 70 expression in the cochlear stria vascularis, which recovered to normal on day 14. Heat shock protein 70 mRNA expression increased in the cochlear stria vascularis following gentamicin ototoxicity, but ligustrazine intervention resulted in decreased levels. CONCLUSION: Ligustrazine significantly ameliorated gentamicin ototoxicity by reducing heat shock protein 70mRNA and protein expression in the cochlear stria vascularis.
关键词	HEAT-SHOCK-PROTEIN RAT COCHLEA EXPRESSION RNA ligustrazine, ototoxicity, gentamicin, heat shock protein 70 cochlea neural regeneration
收录类别	CSCD
语种	英语
资助项目	[National Natural Science Foundation of China] ; [Scientific Research Program of Education Department of Liaoning Province] ; [Science and Technology Foundation of.Liaoning Province]
CSCD记录号	CSCD:3893823
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/146258
专题	中国科学院金属研究所
作者单位	中国科学院金属研究所
推荐引用方式 GB/T 7714	Zhang Liang,Ni Yueqiu,Li Yufang,et al. Ligustrazine facilitates hair cell regeneration in the cochlea following gentamicin ototoxicity[J]. NEURAL REGENERATION RESEARCH,2010,5(10):735-740.
APA	Zhang Liang,Ni Yueqiu,Li Yufang,&Fu Wenshuang.(2010).Ligustrazine facilitates hair cell regeneration in the cochlea following gentamicin ototoxicity.NEURAL REGENERATION RESEARCH,5(10),735-740.
MLA	Zhang Liang,et al."Ligustrazine facilitates hair cell regeneration in the cochlea following gentamicin ototoxicity".NEURAL REGENERATION RESEARCH 5.10(2010):735-740.