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Transplantation of olfactory ensheathing cells promotes axonal regeneration in a rat model of spinal cord injury
Alternative TitleTransplantation of olfactory ensheathing cells promotes axonal regeneration in a rat model of spinal cord injury
Teng Xichuan1; Yoshioka Nozomu1; KimuraKuroda Junko1; Kawamura Koki1; Kawano Hitoshi1; Li Hongpeng1
2010
Source PublicationNEURAL REGENERATION RESEARCH
ISSN1673-5374
Volume5Issue:9Pages:651-656
AbstractBACKGROUND: Transplantation of olfactory ensheathing cells (OECs) into the injured spinal cord has been shown to promote axonal regeneration and functional recovery However, the mechanisms underlying the effects of OEC transplantation remain controversial
Other AbstractBACKGROUND: Transplantation of olfactory ensheathing cells (OECs) into the injured spinal cord has been shown to promote axonal regeneration and functional recovery. However, the mechanisms underlying the effects of OEC transplantation remain controversial. OBJECTIVE: To observe fibrotic scar formation and axonal regeneration in the damaged spinal cord following OEC transplantation, and to determine whether OEC transplantation promotes neural regeneration by attenuating fibrotic scar formation. DESIGN, TIME AND SETTING: A randomized, controlled animal experiment was performed at the Department of Developmental Morphology, Tokyo Metropolitan Institute for Neuroscience, Fuchu, Japan and at the Department of Human Anatomy, College of Basic Medical Sciences, China Medical University, China between April 2007 and May 2009. MATERIALS: OECs were obtained from olfactory nerves and olfactory bulbs of male, 4-week-old, Sprague Dawley rats. Rabbit anti-serotonin polyclonal antibody, rabbit anti-calcitonin gene-related peptide polyclonal antibody, rabbit anti-glial fibrillary acidic protein polyclonal antibody, rabbit anti-type IV collagen polyclonal antibody, and mouse anti-rat endothelial cell antigen-1 monoclonal antibody were used. METHODS: Male, Sprague Dawley rats aged 8 weeks were randomly divided into three groups: sham-surgery (n = 3), surgery (n = 9), and OEC transplantation (n = 11). Spinal cord transection at the T_(9-10) level was performed and the rats were transplanted with a 2-μL (1 × 10~5 cells) cell suspension. MAIN OUTCOME MEASURES: Formation of glial and fibrotic scars was examined using immunohistochemistry for glial fibrillary acidic protein and type IV collagen. Serotonin-positive and calcitonin gene-related peptide-positive axons were visualized by immunohistochemistry, respectively. Double immunofluorescence for type IV collagen and rat endothelial cell antigen-1 was also performed to determine co-localization of type IV collagen deposition and blood vessels. RESULTS: At 1 week after spinal cord injury, numerous glial cells were observed around the lesion site. Formation of fibrotic scar was determined by a large amount of type IV collagen deposition in the lesion center, and descending serotonin- or ascending calcitonin gene-related peptide- containing axons stopped at the fibrotic scar that was formed in the lesion site. At 1 week after transplantation, the formation of fibrotic scar was significantly inhibited. In addition, the fibrotic structure was partly formed and centralized in the blood vessel, and serotonergic and calcitonin gene-related peptide-containing axons were regenerated across the lesion site. CONCLUSION: OEC transplantation into the injured spinal cord attenuated fibrotic scar formation and promoted axon regeneration.
KeywordNIGROSTRIATAL DOPAMINERGIC AXONS FUNCTIONAL RECOVERY SCHWANN-CELLS GLIA TRANSPLANTS FIBROTIC SCAR LESION SITE ADULT RATS INHIBITION fibrotic scar, olfactory ensheathing cell transplantation, spinal cord injury, neural regeneration rats
Indexed ByCSCD
Language英语
Funding Project[National Natural Science Foundation of China] ; [Japan-China Medical Association] ; [Scientific Research Foundation of Technology Department of Liaoning Province in China]
CSCD IDCSCD:3893807
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Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/146392
Collection中国科学院金属研究所
Affiliation1.Tokyo Metropolitan Institute Neurosci, Dept Dev Morphol, Fuchu, Tokyo 1838526, Japan
2.辽宁大学
3.中国科学院金属研究所
Recommended Citation
GB/T 7714
Teng Xichuan,Yoshioka Nozomu,KimuraKuroda Junko,et al. Transplantation of olfactory ensheathing cells promotes axonal regeneration in a rat model of spinal cord injury[J]. NEURAL REGENERATION RESEARCH,2010,5(9):651-656.
APA Teng Xichuan,Yoshioka Nozomu,KimuraKuroda Junko,Kawamura Koki,Kawano Hitoshi,&Li Hongpeng.(2010).Transplantation of olfactory ensheathing cells promotes axonal regeneration in a rat model of spinal cord injury.NEURAL REGENERATION RESEARCH,5(9),651-656.
MLA Teng Xichuan,et al."Transplantation of olfactory ensheathing cells promotes axonal regeneration in a rat model of spinal cord injury".NEURAL REGENERATION RESEARCH 5.9(2010):651-656.
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