Promoting role of an endophyte on the growth and contents of kinsenosides and flavonoids of Anoectochilus formosanus Hayata, a rare and threatened medicinal Orchidaceae plant

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	Promoting role of an endophyte on the growth and contents of kinsenosides and flavonoids of Anoectochilus formosanus Hayata, a rare and threatened medicinal Orchidaceae plant
其他题名	Promoting role of an endophyte on the growth and contents of kinsenosides and flavonoids of Anoectochilus formosanus Hayata, a rare and threatened medicinal Orchidaceae plant *
	Zhang Fusheng 1; Lv Yali 1; Zhao Yue 1; Guo Shunxing 1
	2013
发表期刊	JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
ISSN	1673-1581
卷号	14 期号:9 页码:785-792
摘要	Anoectochilus formosanus, commonly known as "Jewel Orchid", is a Chinese folk medicine used to treat hypertension, diabetes, and heart disease. The existence of A. formosanus is currently threatened by habitat loss, human and animal consumption, etc. The highly potent medicinal activity of A. formosanus is due to its secondary metabolites, especially kinsenosides and flavonoids. This orchid also has a unique mycorrhizal relationship. Most adult orchids rely on endophytes for mineral nutrition and have complex interactions with them, which are related to plant growth, yield and changes in secondary metabolites. This study investigated the promoting role of F-23 fungus (genus Mycena) on the biomass and contents of kinsenosides and flavonoids of A. formosanus in pot culture. The following were observed after 10 weeks of symbiotic cultivation: increased shoot height, shoot dry weight, and leaf numbers by 16.6%, 31.3%, and 22.5%, respectively; increased contents of kinsenosides, isorhamnetin-3-O-beta-D-rutinoside, and isorhamnetin-3-O-beta-D-glucopyranoside by 85.5%, 226.1%, and 196.0%, respectively; some hyphae in epidermal cells dyed red and/or reddish brown by safranine; and, significantly reduced number of starch grains in cortical cells. Moreover, F-23 fungus significantly improved the kinsenoside and flavonoid contents of A. formosanus. These findings supported the reports that endophytes can alter the production of secondary metabolites in their plant hosts, although further physiological, genetic and ecological analyses are warranted.
其他摘要	Anoectochilus formosanus , commonly known as “Jewel Orchid”, is a Chinese folk medicine used to treat hypertension, diabetes, and heart disease. The existence of A. formosanus is currently threatened by habitat loss, human and animal consumption, etc. The highly potent medicinal activity of A. formosanus is due to its secondary metabolites, especially kinsenosides and flavonoids. This orchid also has a unique mycorrhizal relationship. Most adult orchids rely on endophytes for mineral nutrition and have complex interactions with them, which are related to plant growth, yield and changes in secondary metabolites. This study investigated the promoting role of F-23 fungus (genus Mycena ) on the biomass and contents of kinsenosides and flavonoids of A. formosanus in pot culture. The following were observed after 10 weeks of symbiotic cultivation: increased shoot height, shoot dry weight, and leaf numbers by 16.6%, 31.3%, and 22.5%, respectively; increased contents of kinsenosides, isorhamnetin-3- O -β- D -rutinoside, and isorhamnetin-3- O -β- D -glucopyranoside by 85.5%, 226.1%, and 196.0%, respectively; some hyphae in epidermal cells dyed red and A. formosanus . These findings supported the reports that endophytes can alter the production of secondary metabolites in their plant hosts, although further physiological, genetic and ecological analyses are warranted.
关键词	DIABETIC RATS CONSERVATION METABOLISM CULTURE Anoectochilus formosanus Endophyte Secondary metabolite Kinsenoside Flavonoid
收录类别	CSCD
语种	英语
资助项目	[National Natural Science Foundation of China] ; [National High-Tech R&D Program (863) of China]
CSCD记录号	CSCD:4932468
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/146562
专题	中国科学院金属研究所
作者单位	1.Chinese Acad Med Sci, Institute Med Plant Dev, Beijing 100193, Peoples R China Institute Med Plant Dev 2.Peking Union Med Coll, Beijing 100193, Peoples R China 3.山西大学 4.Beijing Chao Yang Hosp, Department Pharmaceut Affairs, Beijing 100020, Peoples R China 5.中国科学院金属研究所
推荐引用方式 GB/T 7714	Zhang Fusheng,Lv Yali,Zhao Yue,et al. Promoting role of an endophyte on the growth and contents of kinsenosides and flavonoids of Anoectochilus formosanus Hayata, a rare and threatened medicinal Orchidaceae plant[J]. JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B,2013,14(9):785-792.
APA	Zhang Fusheng,Lv Yali,Zhao Yue,&Guo Shunxing.(2013).Promoting role of an endophyte on the growth and contents of kinsenosides and flavonoids of Anoectochilus formosanus Hayata, a rare and threatened medicinal Orchidaceae plant.JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B,14(9),785-792.
MLA	Zhang Fusheng,et al."Promoting role of an endophyte on the growth and contents of kinsenosides and flavonoids of Anoectochilus formosanus Hayata, a rare and threatened medicinal Orchidaceae plant".JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B 14.9(2013):785-792.