Experimental study on enhancement of the metastatic potential of portal vein tumor thrombus-originated hepatocellular carcinoma cells using portal vein serum

IMR OpenIR

	Experimental study on enhancement of the metastatic potential of portal vein tumor thrombus-originated hepatocellular carcinoma cells using portal vein serum
其他题名	Experimental study on enhancement of the metastatic potential of portal vein tumor thrombus-originated hepatocellular carcinoma cells using portal vein serum
	Tang Yufu 1; Yu Hongming 1; Zhang Long 1; Wang Kang 1; Guo Weixing 1; Shi Jie 1; Liu Shupeng 3; Wu Mengchao 1; Wang Hongyang 4; Cheng Shuqun 1
	2014
发表期刊	CHINESE JOURNAL OF CANCER RESEARCH
ISSN	1000-9604
卷号	26 期号:5 页码:588-595
摘要	Objective: Portal vein metastasis of hepatocellular carcinoma (HCC) results in a poor prognosis and seriously affects the survival rate of patients. The mechanism underlying the formation of portal vein tumor thrombus (PVTT) is complex and is not yet fully understood. This study was conducted to investigate the impact of portal vein blood on the proliferation, metastasis, invasion and apoptosis of PVTT cells and to explore its possible mechanisms, which was expected to lay a foundation for ascertaining the mechanism underlying the portal vein metastasis of HCC.
其他摘要	Objective Portal vein metastasis of hepatocellular carcinoma (HCC) results in a poor prognosis and seriously affects the survival rate of patients. The mechanism underlying the formation of portal vein tumor thrombus (PVTT) is complex and is not yet fully understood. This study was conducted to investigate the impact of portal vein blood on the proliferation, metastasis, invasion and apoptosis of PVTT cells and to explore its possible mechanisms, which was expected to lay a foundation for ascertaining the mechanism underlying the portal vein metastasis of HCC. Methods Peripheral blood and portal vein blood were collected from patients with HCC, and the sera from these two sources were used to culture the PVTT-originated HCC cell line CSQT-2. The cells were collected after 24 h, and flow cytometry was performed to detect cell proliferation, cell cycle stages and apoptosis. Transwell migration and invasion assays were applied to detect the metastasis and invasion of the cells in each group. The changes in the expression of MMP-2 and MMP-9 in cells were detected via Western blotting. The contents of IL-12, IFN-γ, IL-1β, IL-2 and TNF-α in the two groups of sera were quantified using corresponding kits. Results Compared with the group of cells cultured with peripheral serum, the cells cultured with portal vein serum showed significantly lower apoptosis (P<0.01), significantly enhanced cell metastasis and invasion (P<0.01), whereas cell proliferation and the stages of the cell cycle did not differ significantly (P>0.05). A significantly increased expression level of MMP-2 has been observed in tumor cells treated portal vein serum. In addition, compared with peripheral serum, the content of IL-12 was significantly decreased in portal vein serum (P<0.05), while the contents of IFN-γ, IL-1β, IL-2, and TNF-α did not differ significantly (P>0.05). Conclusions Portal vein serum from HCC patients could inhibit the apoptosis of PVTT-originated HCC cells and promote cell metastasis and invasion. This effect may be related to the lower level of IL-12 in portal vein serum.
关键词	EXPRESSION METALLOPROTEINASES MICROENVIRONMENT HEPATECTOMY MANAGEMENT SURVIVAL INVASION MATRIX CANCER IL-12 Hepatocellular carcinoma (HCC) portal vein portal vein tumor thrombus (PVTT) metastasis
收录类别	CSCD
语种	英语
资助项目	[China National Funds for the Science Fund for Creative Research Groups] ; [State Key Project on Infections Diseases of China] ; [China National Funds for the Distinguished Young Scientists] ; [National Natural Science Foundation] ; [New Excellent Talents Program of Shanghai Municipal Health Bureau] ; [Shanghai Science and Technology Committee] ; [Program for New Century Excellent Talents in University] ; [SMMU Innovation Alliance for Liver Cancer Diagnosis and Treatment] ; [Chang Jiang Scholars Program]
CSCD记录号	CSCD:5281615
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/146802
专题	中国科学院金属研究所
作者单位	1.Second Mil Med University, Eastern Hepatobiliary Surg Hosp, Shanghai 200438, Peoples R China 2.中国科学院金属研究所 3.Second Mil Med University, Changhai Hosp, Shanghai 200433, Peoples R China 4.第二军医大学
推荐引用方式 GB/T 7714	Tang Yufu,Yu Hongming,Zhang Long,et al. Experimental study on enhancement of the metastatic potential of portal vein tumor thrombus-originated hepatocellular carcinoma cells using portal vein serum[J]. CHINESE JOURNAL OF CANCER RESEARCH,2014,26(5):588-595.
APA	Tang Yufu.,Yu Hongming.,Zhang Long.,Wang Kang.,Guo Weixing.,...&Cheng Shuqun.(2014).Experimental study on enhancement of the metastatic potential of portal vein tumor thrombus-originated hepatocellular carcinoma cells using portal vein serum.CHINESE JOURNAL OF CANCER RESEARCH,26(5),588-595.
MLA	Tang Yufu,et al."Experimental study on enhancement of the metastatic potential of portal vein tumor thrombus-originated hepatocellular carcinoma cells using portal vein serum".CHINESE JOURNAL OF CANCER RESEARCH 26.5(2014):588-595.