Lipopolysaccharide-activated microglial-induced neuroglial cell differentiation in bone marrow mesenchymal stem cells

IMR OpenIR

	Lipopolysaccharide-activated microglial-induced neuroglial cell differentiation in bone marrow mesenchymal stem cells
其他题名	Lipopolysaccharide-activated microglial-induced neuroglial cell differentiation in bone marrow mesenchymal stem cells
	Luo Xiaoguang; Ge Chunlin; Ren Yan; Yu Hongmei; Wu Zhe; Wang Qiushuang; Zhang Chaodong
	2008
发表期刊	NEURAL REGENERATION RESEARCH
ISSN	1673-5374
卷号	3 期号:3 页码:241-244
摘要	BACKGROUND: Microglia are very sensitive to environmental changes, often becoming activated by pathological conditions. Activated microglia can exert it dual role in injury and repair in various diseases of the central nervous system, including cerebral ischemia, Parkinson's disease, and mesenchymalAlzheimer's disease.
其他摘要	BACKGROUND: Microglia are very sensitive to environmental changes, often becoming activated by pathological conditions. Activated microglia can exert a dual role in injury and repair in various diseases of the central nervous system, including cerebral ischemia, Parkinson's disease, and Alzheimer's disease. OBJECTIVE: An immortal microglial cell line, BV2, was treated with varying concentrations of lipopolysaccharide (LPS) to induce a pathological situation. Supernatant was harvested and incubated with bone marrow mesenchymal stem cells and, concomitantly, bone marrow mesenchymal stem cell differentiation was observed. DESIGN: A controlled observation, in vitro experiment. SETTING: Department of Neurology, First Affiliated Hospital of China Medical University. MATERIALS: Five male 2-3-week-old Sprague Dawley rats were purchased from Animal Laboratory Center of China Medical University and included in this study. The protocol was performed in accordance with ethical guidelines for the use and care of animals. The microglial cell line BV2 was produced by Cell Research Institute of Chinese Academy of Sciences. LPS was produced by Sigma Company, USA. METHODS: This study was performed in the Central Laboratory of China Medical University from September 2006 to March 2007. Rat femoral and tibial bone marrow was collected for separation and primary culture of bone marrow mesenchymal stem cells. Bone marrow mesenchymal stem cell cultures were divided into 5 groups: control group, non-activated group, as well as low-, medium-, and high-dose LPS groups. In the control group, bone marrow mesenchymal stem cells were cultured with Dulbecco's modified Eagle's medium (DMEM) supplemented with fetal bovine serum (volume fraction 0.1). In the non-activated group, bone marrow mesenchymal stem cells were incubated with non-activated BV2 supernatant. In the low-, medium-, and high-dose LPS groups, bone marrow mesenchymal stem cells were incubated with LPS (0.01, 0.1 and 1 μg/L, respectively)-activated BV2 supernatant. MAIN OUTCOME MEASURES: Expression of glial fibrillary acidic protein (GFAP) and neuron-specific enolase (NSE) in bone marrow mesenchymal stem cells was detected by immunofluorescence staining.RESULTS: GFAP-positive cells were detected in each group; however, the greatest number were found in the high-dose LPS group. The number of GFAP-positive cells was significantly greater in the high- and medium-dose groups, compared to the control, non-activated, and low-dose LPS groups (P 0.05). NSE-positive cells were also detected in each group. However, there was no significant difference between any two groups (P > 0.05).CONCLUSION: Microglia, when activated to some ertent, could induce neuroglial cell differentiation from bone marrow mesenchymal stem cells; however, they did not exhibit the capacity to markedly promote neuronal cell differentiation. When microglia are activated, the capacity to induce bone marrow mesenchymal stem cell differentiation reaches a peak level, but is not increased with greater activation rates.
关键词	CENTRAL-NERVOUS-SYSTEM BRAIN DISEASE INJURY BETA MANIFESTATIONS INFILTRATION MODULATION SENESCENCE PHENOTYPE BV2 bone marrow mesenchymal stem cells microglia lipopolysaccharide
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:3299701
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/147545
专题	中国科学院金属研究所
作者单位	中国科学院金属研究所
推荐引用方式 GB/T 7714	Luo Xiaoguang,Ge Chunlin,Ren Yan,et al. Lipopolysaccharide-activated microglial-induced neuroglial cell differentiation in bone marrow mesenchymal stem cells[J]. NEURAL REGENERATION RESEARCH,2008,3(3):241-244.
APA	Luo Xiaoguang.,Ge Chunlin.,Ren Yan.,Yu Hongmei.,Wu Zhe.,...&Zhang Chaodong.(2008).Lipopolysaccharide-activated microglial-induced neuroglial cell differentiation in bone marrow mesenchymal stem cells.NEURAL REGENERATION RESEARCH,3(3),241-244.
MLA	Luo Xiaoguang,et al."Lipopolysaccharide-activated microglial-induced neuroglial cell differentiation in bone marrow mesenchymal stem cells".NEURAL REGENERATION RESEARCH 3.3(2008):241-244.