High-quality total RNA extraction from Magnolia sieboldii K. Koch seeds: a comparative evaluation

IMR OpenIR

	High-quality total RNA extraction from Magnolia sieboldii K. Koch seeds: a comparative evaluation
	Wei Jun 1; Zhang Xiaolin 1; Hou Zhe 2; Lu Xiujun 1
	2019
发表期刊	JOURNAL OF FORESTRY RESEARCH
ISSN	1007-662X
卷号	30 期号:1 页码:371-379
摘要	Magnolia sieboldii K. Koch seed is characterized with having deep dormancy. The inner molecular regulation mechanism has not been investigated because of the absence of a protocol for total RNA (ribonucleic acid) extraction. The extraction of high-quality RNA is important and can be a limiting factor in plant molecular biology experiments. Sufficient high-quality total RNA is required to elucidate the molecular regulation mechanism of germination. However, M. sieboldii seeds with large amounts of secondary metabolites also contain recalcitrant tissues for RNA isolation. We found two simple and low-cost RNA extraction methods for M. sieboldii seeds by evaluating and selecting eight types of methods and further optimizing these methods. The two methods were not only suitable for extracting M. sieboldii seed RNA but also applicable to RNAs from several other tissues. Total RNA extracted through these approaches was applicable for general molecular biology experiments such as qRT-PCR (quantitative real-time polymerase chain reactions). The protocols also meet the strict harsh requirements for transcriptome sequencing and small RNA sequencing. This study provides a powerful approach for future studies at the transcription level.
关键词	ESTABLISHMENT Seed dormancy RNA isolation qRT-PCR Small RNA Magnolia sieboldii
收录类别	CSCD
语种	英语
资助项目	[National Natural Science Foundation of China]
CSCD记录号	CSCD:6424041
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/147711
专题	中国科学院金属研究所
作者单位	1.中国科学院金属研究所 2.Chinese Acad Forestry, Res Institute Forestry, 1 Xiangshan Rd, Beijing 100091, Peoples R China Res Institute Forestry
推荐引用方式 GB/T 7714	Wei Jun,Zhang Xiaolin,Hou Zhe,et al. High-quality total RNA extraction from Magnolia sieboldii K. Koch seeds: a comparative evaluation[J]. JOURNAL OF FORESTRY RESEARCH,2019,30(1):371-379.
APA	Wei Jun,Zhang Xiaolin,Hou Zhe,&Lu Xiujun.(2019).High-quality total RNA extraction from Magnolia sieboldii K. Koch seeds: a comparative evaluation.JOURNAL OF FORESTRY RESEARCH,30(1),371-379.
MLA	Wei Jun,et al."High-quality total RNA extraction from Magnolia sieboldii K. Koch seeds: a comparative evaluation".JOURNAL OF FORESTRY RESEARCH 30.1(2019):371-379.