Akt Gene Therapy Enhances Angiogenesis in Ischemic Hind Limb

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	Akt Gene Therapy Enhances Angiogenesis in Ischemic Hind Limb
其他题名	Akt Gene Therapy Enhances Angiogenesis in Ischemic Hind Limb
	Huo Xin; Zhang Qiang; Liu Ying
	2010
发表期刊	CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
ISSN	1005-9040
卷号	26 期号:3 页码:409-414
摘要	Both bone marrow-derived mesenchymal stem cells(MSCs) therapy and gene therapy have been applied to animal studies and clinical trials. The goal of this study was to compare the effects of three types of MSCs transplantation. In the first part of the study, MSCs were transduced with the pEGFP-C1 and transfected with the pEGFP-C1/Akt in a model of bilateral hind limb ischemia in rats. Following gene transduction, the production of Akt and VEGF protein was more in the culture media of GFP-Akt/MSCs group than that in those of the GFP/MSCs group. In the second part of the study, Sprague-Dawley rats(n=10/group) underwent surgery to create bilateral hind limb ischemia and were randomized into two groups consisting of a GFP/Akt-MSCs group(MSCs suspension, 1x10(7) MSCs/100 mu L transfected pEGFP-C1/Akt) and a GFP-MSCs group(MSCs suspension, 1x10(7) MSCs/100 mu L). These PEGFP-C1/Akt and PEGFP-C1 transfected MSCs suspensions were slowly infused into the adductor muscles of the rat's left hind limb, while the rat's right hind limb in the control group received an equal volume of PBS. Endpoints includes angiographic analysis, evaluation of capillary density, immunohistochemistry for von Willebrand factor immunodetection of Akt and VEGF protein, RT-PCR of VEGF and Akt mRNA levels in vitro and in vivo. Our data indicate that the tissue perfusion can improve capillary density and the mature of vasculature in the GFP-Akt/MSCs group compared to that in the GFP-MSCs group or control group in the rat model of bilateral hind limb ischemia. Transplantation of MSCs transfected with Akt gene may become the future therapy for hind limb ischemia.
其他摘要	Both bone marrow-derived mesenchymal stem cells(MSCs) therapy and gene therapy have been applied to animal studies and clinical trials. The goal of this study was to compare the effects of three types of MSCs transplantation. In the first part of the study, MSCs were transduced with the pEGFP-C1 and transfected with the pEGFP-C1/Akt in a model of bilateral hind limb ischemia in rats. Following gene transduction, the production of Akt and VEGF protein was more in the culture media of GFP-Akt/MSCs group than that in those of the GFP/MSCs group. In the second part of the study, Sprague-Dawley rats(n=10/group) underwent surgery to create bilateral hind limb ischemia and were randomized into two groups consisting of a GFP/Akt-MSCs group(MSCs suspension, 1×10~7 MSCs/100 μL transfected pEGFP-C1/Akt) and a GFP-MSCs group(MSCs suspension, 1×10~7 MSCs/100 μL). These PEGFP-C1/Akt and PEGFP-C1 transfected MSCs suspensions were slowly infused into the adductor muscles of the rat’s left hind limb, while the rat’s right hind limb in the control group received an equal volume of PBS. Endpoints includes angiographic analysis, evaluation of capillary density, immunohistochemistry for von Willebrand factor (vWF), immunodetection of Akt and VEGF protein, RT-PCR of VEGF and Akt mRNA levels in vitro and in vivo. Our data indicate that the tissue perfusion can improve capillary density and the mature of vasculature in the GFP-Akt/MSCs group compared to that in the GFP-MSCs group or control group in the rat model of bilateral hind limb ischemia. Transplantation of MSCs transfected with Akt gene may become the future therapy for hind limb ischemia.
关键词	MESENCHYMAL STEM-CELLS PROMOTES ANGIOGENESIS DISEASE Bone mesenchymal stem cell pEGFP-C1/Akt Hindlimb Ischemia Angiogenesis
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:3893166
引用统计	被引频次：1[CSCD] [CSCD记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/147777
专题	中国科学院金属研究所
作者单位	中国科学院金属研究所
推荐引用方式 GB/T 7714	Huo Xin,Zhang Qiang,Liu Ying. Akt Gene Therapy Enhances Angiogenesis in Ischemic Hind Limb[J]. CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,2010,26(3):409-414.
APA	Huo Xin,Zhang Qiang,&Liu Ying.(2010).Akt Gene Therapy Enhances Angiogenesis in Ischemic Hind Limb.CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,26(3),409-414.
MLA	Huo Xin,et al."Akt Gene Therapy Enhances Angiogenesis in Ischemic Hind Limb".CHEMICAL RESEARCH IN CHINESE UNIVERSITIES 26.3(2010):409-414.