Effect of dl-praeruptorin A on desmin and vimentin content in rat ischemia/reperfusion myocardiocytes

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	Effect of dl-praeruptorin A on desmin and vimentin content in rat ischemia/reperfusion myocardiocytes
其他题名	Effect of dl-praeruptorin A on desmin and vimentin content in rat ischemia/reperfusion myocardiocytes
	Chang He 1; Chu Xiaoyu 2; Zou Jun 3; Chang Tianhui 2
	2007
发表期刊	CHINESE MEDICAL JOURNAL
ISSN	0366-6999
卷号	120 期号:24 页码:2256-2259
摘要	Background Intermediate filament (IF) proteins have been thought to play a role in nuclear centration, organelle movement and maintenance of cell shape. di-praeruptorin A (Pd-la), a novel Ca2+-influx blocker and K+-channel opener isolated from Chinese traditional herbal medicine Qian-Hu, has been demonstrated to inhibit expression of apoptosis related proteins and reduce the level of proinflammatory factors in ischemia/reperfusion myocardiocytes. Morphologically, whether Pd-la effects myocardiocyte IFs remains unclear. The purpose of this study was, for the first time, to evaluate the in vivo effects of Pd-la on IF desmin and vimentin content in order to further explore its cardioprotection against ischemia and elucidate its mechanism of action.
其他摘要	Background Intermediate filament (IF) proteins have been thought to play a role in nuclear centration, organelle movement and maintenance of cell shape. dl-praeruptorin A (Pd-Ia), a novel Ca2+-influx blocker and K+-channel opener isolated from Chinese traditional herbal medicine Qian-Hu, has been demonstrated to inhibit expression of apoptosis related proteins and reduce the level of proinflammatory factors in ischemia/reperfusion myocardiocytes. Morphologically, whether Pd-Ia effects myocardiocyte IFs remains unclear. The purpose of this study was, for the first time, to evaluate the in vivo effects of Pd-Ia on IF desmin and vimentin content in order to further explore its cardioprotection against ischemia and elucidate its mechanism of action. Methods Rats underwent a 30 minutes coronary occlusion followed by 120 minutes reperfusion. Assessment of desmin and vimentin content of myocardiocytes was performed by immunohistochemistry, Western blot, Hematoxylin-Eosin staining and densitometry.Results Pretreatment with i.v. infusion of Pd-Ia prior to ischemia significantly increased desmin and vimentin content and alleviated defects caused by the ischemia/reperfusion insult, e.g. with Pd-Ia at a dose of 0.5 or 1.0 mg/kg, integrated density values of desmin were increased from 61?478 ± 10?074 to 177 408 ± 10?395 and 195?784 ± 20?057, and vimentin from 59?189 ± 19?853 to 164?781 ± 19?543 and 185?696 ± 20?957 (P＜0.01, vs placebo), respectively. The recovery of desmin seemed to be stronger and appeared earlier than that of vimentin. Conclusion Pd-Ia protectively increased IF desmin and vimentin content in ischemia/reperfusion myocardiocytes, which might be partially responsible for its cardioprotection against ischemia.
关键词	NECROSIS-FACTOR-ALPHA FACTOR-KAPPA-B PROTEIN DYSFUNCTION EXPRESSION HEART dl-praeruptorin A desmin vimentin intermediate filament ischemia myocardiocyte
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:3189357
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/148010
专题	中国科学院金属研究所
作者单位	1.新潟大学 2.中国科学院金属研究所 3.厦门大学
推荐引用方式 GB/T 7714	Chang He,Chu Xiaoyu,Zou Jun,et al. Effect of dl-praeruptorin A on desmin and vimentin content in rat ischemia/reperfusion myocardiocytes[J]. CHINESE MEDICAL JOURNAL,2007,120(24):2256-2259.
APA	Chang He,Chu Xiaoyu,Zou Jun,&Chang Tianhui.(2007).Effect of dl-praeruptorin A on desmin and vimentin content in rat ischemia/reperfusion myocardiocytes.CHINESE MEDICAL JOURNAL,120(24),2256-2259.
MLA	Chang He,et al."Effect of dl-praeruptorin A on desmin and vimentin content in rat ischemia/reperfusion myocardiocytes".CHINESE MEDICAL JOURNAL 120.24(2007):2256-2259.