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Effects of transferred NK4 gene on proliferation, migration, invasion and apoptosis of human prostate cancer DU145 cells
Alternative TitleEffects of transferred NK4 gene on proliferation, migration, invasion and apoptosis of human prostate cancer DU145 cells
Yue Dan; Wang Yong; Ma Ping; Li YinYan; Chen Hong; Wang Ping; Ren ChangShan
2010
Source PublicationASIAN JOURNAL OF ANDROLOGY
ISSN1008-682X
Volume12Issue:3Pages:381-389
AbstractWe investigated the ability of NK4, an antagonist of human hepatocyte growth factor (HGF), to inhibit the influence of HGF on proliferation, migration, invasion and apoptosis of human prostate cancer cells. Expression vector pBudCE4.1-EGFP-NK4 containing NK4 cDNA was used to transfect human prostate cancer DU145 cells, and the effects of the autocrine NK4 on tumor cell proliferation, migration, invasion and apoptosis were assessed in vitro. In vivo, we subcutaneously implanted DU145 cells, mock-transfected clone (DU145/empty vector) cells and NK4-transfected clone (DU145/NK4) cells into nude mice, and then evaluated tumor growth, cell proliferation and cell apoptosis in vivo. We found that DU145/NK4 cells expressed NK4 protein. In the in vitro study, autocrine NK4 attenuated the HGF-induced tumor cell proliferation, migration and invasion, and stimulated apoptosis. Furthermore, autocrine NK4 effectively inhibited the HGF-induced phosphorylation of c-Met, extracellular signal-regulated kinase-1 (ERK1). and protein kinase B 1/2 (Akt1/2). Histological examination revealed that autocrine NK4 inhibited proliferation and accelerated apoptosis of prostate cancer cells. These results show that genetic modification of DU145 cells with NK4 cDNA yields a significant effect on their proliferation, migration, invasion and apoptosis. Molecular targeting of HGF/c-Met by NK4 could be applied as a novel therapeutic approach to prostate cancer.
Other AbstractWe investigated the ability of NK4, an antagonist of human hepatocyte growth factor (HGF), to inhibit the influence of HGF on proliferation, migration, invasion and apoptosis of human prostate cancer cells. Expression vector pBudCE4.1-EGFP-NK4 containing NK4 cDNA was used to transfect human prostate cancer DU145 cells, and the effects of the autocrine NK4 on tumor cell proliferation, migration, invasion and apoptosis were assessed in vitro . In vivo , we subcutaneously implanted DU145 cells, mock-transfected clone (DU145 in vivo . We found that DU145 in vitro study, autocrine NK4 attenuated the HGF-induced tumor cell proliferation, migration and invasion, and stimulated apoptosis. Furthermore, autocrine NK4 effectively inhibited the HGF-induced phosphorylation of c-Met, extracellular signal-regulated kinase-1 (ERK1). and protein kinase B 1
KeywordHEPATOCYTE GROWTH-FACTOR RECEPTOR C-MET NUDE-MICE HGF ANGIOGENESIS METASTASIS ANTAGONIST BENIGN EXPRESSION CARCINOMA hepatocyte growth factor human prostate cancer NK4 DU145 cells
Indexed ByCSCD
Language英语
CSCD IDCSCD:3891332
Citation statistics
Cited Times:6[CSCD]   [CSCD Record]
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/148566
Collection中国科学院金属研究所
Affiliation中国科学院金属研究所
Recommended Citation
GB/T 7714
Yue Dan,Wang Yong,Ma Ping,et al. Effects of transferred NK4 gene on proliferation, migration, invasion and apoptosis of human prostate cancer DU145 cells[J]. ASIAN JOURNAL OF ANDROLOGY,2010,12(3):381-389.
APA Yue Dan.,Wang Yong.,Ma Ping.,Li YinYan.,Chen Hong.,...&Ren ChangShan.(2010).Effects of transferred NK4 gene on proliferation, migration, invasion and apoptosis of human prostate cancer DU145 cells.ASIAN JOURNAL OF ANDROLOGY,12(3),381-389.
MLA Yue Dan,et al."Effects of transferred NK4 gene on proliferation, migration, invasion and apoptosis of human prostate cancer DU145 cells".ASIAN JOURNAL OF ANDROLOGY 12.3(2010):381-389.
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