Nitric oxide induced by polyamines involves antioxidant systems against chilling stress in tomato (Lycopersicon esculentum Mill.) seedling

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	Nitric oxide induced by polyamines involves antioxidant systems against chilling stress in tomato (Lycopersicon esculentum Mill.) seedling
其他题名	Nitric oxide induced by polyamines involves antioxidant systems against chilling stress in tomato ( Lycopersicon esculentum Mill.) seedling * #
	Diao Qiannan; Song Yongjun; Shi Dongmei; Qi Hongyan
	2016
发表期刊	JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
ISSN	1673-1581
卷号	17 期号:12 页码:916-930
摘要	Polyamines (PAs) and nitric oxide (NO) are vital signals in modulating plant response to abiotic stress. However, to our knowledge, studies on the relationship between NO and PAs in response to cold stress in tomato are limited. Accordingly, in this study, we investigated the effects of putrescine (Put) and spermidine (Spd) on NO generation and the function of Spd-induced NO in the tolerance of tomato seedling under chilling stress. Spd increased NO release via the nitric oxide synthase (NOS)-like and nitrate reductase (NR) enzymatic pathways in the seedlings, whereas Put had no such effect. Moreover, H2O2 might act as an upstream signal to stimulate NO production. Both exogenous NO donor (sodium nitroprusside (SNP)) and Spd enhanced chilling tolerance in tomato, thereby protecting the photosynthetic system from damage. Compared to chilling treatment alone, Spd enhanced the gene expressions of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX), and their enzyme activities in tomato leaves. However, a scavenger or inhibitor of NO abolished Spd-induced chilling tolerance and blocked the increased expression and activity due to Spd of these antioxidant enzymes in tomato leaves under chilling stress. The results showed that NO induced by Spd plays a crucial role in tomato's response to chilling stress.
其他摘要	Polyamines (PAs) and nitric oxide (NO) are vital signals in modulating plant response to abiotic stress. However, to our knowledge, studies on the relationship between NO and PAs in response to cold stress in tomato are limited. Accordingly, in this study, we investigated the effects of putrescine (Put) and spermidine (Spd) on NO generation and the function of Spd-induced NO in the tolerance of tomato seedling under chilling stress. Spd increased NO release via the nitric oxide synthase (NOS)-like and nitrate reductase (NR) enzymatic pathways in the seedlings, whereas Put had no such effect. Moreover, H 2 O 2 might act as an upstream signal to stimulate NO production. Both exogenous NO donor (sodium nitroprusside (SNP)) and Spd enhanced chilling tolerance in tomato, thereby protecting the photosynthetic system from damage. Compared to chilling treatment alone, Spd enhanced the gene expressions of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX), and their enzyme activities in tomato leaves. However, a scavenger or inhibitor of NO abolished Spd-induced chilling tolerance and blocked the increased expression and activity due to Spd of these antioxidant enzymes in tomato leaves under chilling stress. The results showed that NO induced by Spd plays a crucial role in tomato’s response to chilling stress.
关键词	INDUCED OXIDATIVE DAMAGE HYDROGEN-PEROXIDE ABIOTIC STRESS COLD-ACCLIMATION LOW-TEMPERATURE CELL-DEATH CHLOROPHYLL FLUORESCENCE PHYSIOLOGICAL STRATEGIES ARABIDOPSIS-THALIANA ASCORBATE PEROXIDASE Antioxidant enzymes Chilling tolerance Hydrogen peroxide Nitric oxide Spermidine Tomato
收录类别	CSCD
语种	英语
资助项目	[China Agriculture Research System] ; [Liaoning Innovative Research Team in University, China]
CSCD记录号	CSCD:5879956
引用统计	被引频次：5[CSCD] [CSCD记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/149303
专题	中国科学院金属研究所
作者单位	中国科学院金属研究所
推荐引用方式 GB/T 7714	Diao Qiannan,Song Yongjun,Shi Dongmei,et al. Nitric oxide induced by polyamines involves antioxidant systems against chilling stress in tomato (Lycopersicon esculentum Mill.) seedling[J]. JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B,2016,17(12):916-930.
APA	Diao Qiannan,Song Yongjun,Shi Dongmei,&Qi Hongyan.(2016).Nitric oxide induced by polyamines involves antioxidant systems against chilling stress in tomato (Lycopersicon esculentum Mill.) seedling.JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B,17(12),916-930.
MLA	Diao Qiannan,et al."Nitric oxide induced by polyamines involves antioxidant systems against chilling stress in tomato (Lycopersicon esculentum Mill.) seedling".JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B 17.12(2016):916-930.