| Nitric oxide induced by polyamines involves antioxidant systems against chilling stress in tomato (Lycopersicon esculentum Mill.) seedling | |
| Alternative Title | Nitric oxide induced by polyamines involves antioxidant systems against chilling stress in tomato ( Lycopersicon esculentum Mill.) seedling * # |
| Diao Qiannan; Song Yongjun; Shi Dongmei; Qi Hongyan | |
| 2016 | |
| Source Publication | JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
 |
| ISSN | 1673-1581 |
| Volume | 17Issue:12Pages:916-930 |
| Abstract | Polyamines (PAs) and nitric oxide (NO) are vital signals in modulating plant response to abiotic stress. However, to our knowledge, studies on the relationship between NO and PAs in response to cold stress in tomato are limited. Accordingly, in this study, we investigated the effects of putrescine (Put) and spermidine (Spd) on NO generation and the function of Spd-induced NO in the tolerance of tomato seedling under chilling stress. Spd increased NO release via the nitric oxide synthase (NOS)-like and nitrate reductase (NR) enzymatic pathways in the seedlings, whereas Put had no such effect. Moreover, H2O2 might act as an upstream signal to stimulate NO production. Both exogenous NO donor (sodium nitroprusside (SNP)) and Spd enhanced chilling tolerance in tomato, thereby protecting the photosynthetic system from damage. Compared to chilling treatment alone, Spd enhanced the gene expressions of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX), and their enzyme activities in tomato leaves. However, a scavenger or inhibitor of NO abolished Spd-induced chilling tolerance and blocked the increased expression and activity due to Spd of these antioxidant enzymes in tomato leaves under chilling stress. The results showed that NO induced by Spd plays a crucial role in tomato's response to chilling stress. |
| Other Abstract | Polyamines (PAs) and nitric oxide (NO) are vital signals in modulating plant response to abiotic stress. However, to our knowledge, studies on the relationship between NO and PAs in response to cold stress in tomato are limited. Accordingly, in this study, we investigated the effects of putrescine (Put) and spermidine (Spd) on NO generation and the function of Spd-induced NO in the tolerance of tomato seedling under chilling stress. Spd increased NO release via the nitric oxide synthase (NOS)-like and nitrate reductase (NR) enzymatic pathways in the seedlings, whereas Put had no such effect. Moreover, H 2 O 2 might act as an upstream signal to stimulate NO production. Both exogenous NO donor (sodium nitroprusside (SNP)) and Spd enhanced chilling tolerance in tomato, thereby protecting the photosynthetic system from damage. Compared to chilling treatment alone, Spd enhanced the gene expressions of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX), and their enzyme activities in tomato leaves. However, a scavenger or inhibitor of NO abolished Spd-induced chilling tolerance and blocked the increased expression and activity due to Spd of these antioxidant enzymes in tomato leaves under chilling stress. The results showed that NO induced by Spd plays a crucial role in tomato’s response to chilling stress. |
| Keyword | INDUCED OXIDATIVE DAMAGE HYDROGEN-PEROXIDE ABIOTIC STRESS COLD-ACCLIMATION LOW-TEMPERATURE CELL-DEATH CHLOROPHYLL FLUORESCENCE PHYSIOLOGICAL STRATEGIES ARABIDOPSIS-THALIANA ASCORBATE PEROXIDASE Antioxidant enzymes Chilling tolerance Hydrogen peroxide Nitric oxide Spermidine Tomato |
| Indexed By | CSCD |
| Language | 英语 |
| Funding Project | [China Agriculture Research System] ; [Liaoning Innovative Research Team in University, China] |
| CSCD ID | CSCD:5879956 |
| Citation statistics |
Cited Times:5[CSCD]
[CSCD Record]
|
| Document Type | 期刊论文 |
| Identifier | http://ir.imr.ac.cn/handle/321006/149303 |
| Collection | 中国科学院金属研究所 |
| Affiliation | 中国科学院金属研究所 |
| Recommended Citation GB/T 7714 | Diao Qiannan,Song Yongjun,Shi Dongmei,et al. Nitric oxide induced by polyamines involves antioxidant systems against chilling stress in tomato (Lycopersicon esculentum Mill.) seedling[J]. JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B,2016,17(12):916-930. |
| APA | Diao Qiannan,Song Yongjun,Shi Dongmei,&Qi Hongyan.(2016).Nitric oxide induced by polyamines involves antioxidant systems against chilling stress in tomato (Lycopersicon esculentum Mill.) seedling.JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B,17(12),916-930. |
| MLA | Diao Qiannan,et al."Nitric oxide induced by polyamines involves antioxidant systems against chilling stress in tomato (Lycopersicon esculentum Mill.) seedling".JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B 17.12(2016):916-930. |
| Files in This Item: | There are no files associated with this item. | |||||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment