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Changes in intracellular calcium in brain cells of aged rats
Alternative TitleChanges in intracellular calcium in brain cells of aged rats
Li Yu; Cao Yunpeng
2008
Source PublicationNEURAL REGENERATION RESEARCH
ISSN1673-5374
Volume3Issue:9Pages:1026-1029
AbstractBACKGROUND: Studies have shown that voltage-dependent calcium influx, and enhancement of certain calcium-dependent processes in neurons, is related to aging.
Other AbstractBACKGROUND:Studies have shown that voltage-dependent calcium influx,and enhancement of certain calcium-dependent processes in neurons,is related to aging.OBJECTIVE:To observe changes in intracellular calcium(Ca~(2+)_i) in neurons of aged rats,and to compare with young rats.DESIGN,TIME AND SETTING:A randomized control experiment of neurophysiology was performed at the Central Laboratory of School of Pharmaceutical Science,China Medical University from June to August 2004.MATERIALS:Ten male,healthy,Wistar rats,19 months old,were selected for the aged group.Ten male,3-month-old,Wistar rats were selected for the young control group.Fura-2/AM was provided by the Institute of Pharmaceutical Research of Chinese Academy of Medical Sciences,and the F-2000 fluorospectrophotometer was a product of Hitachi,Japan.METHODS:Fluorescence Fura-2 spectrophotometer was used to measure Ca~(2+)_i in acutely dissociated brain cells of aged and young rats.The concentration of extracellular potassium was controlled by adding different volumes of chloridated potassium solution of high concentration.MAIN OUTCOME MEASURES:Ca~(2+)_i in neurons of young and aged rats in the presence of 1 mmol/L extracellular calcium concentration and 0 mmol/L(resting state),5,10,20,and 40 mmol/L extracellular potassium.Absolute increase of Ca~(2+)_i in neurons of young and aged rats when extracellular potassium was 5,10,20,40 mmol/L.RESULTS:In the presence of 1 mmol/L extracellular Ca2+ and 0 mmol/L(resting state),5,10,20,and 40 mmol/L extracellular potassium,Ca~(2+)_i in the neurons of aged rats was significantly less than that in young rats(P 0.05).CONCLUSION:The overload of Ca~(2+)_i in neurons of aged rats is greater than that of young rats under the same circumstances.
KeywordHIPPOCAMPAL CA1 NEURONS SYNAPTIC PLASTICITY ALZHEIMERS-DISEASE SLOW AFTERHYPERPOLARIZATION ENDOPLASMIC-RETICULUM PYRAMIDAL NEURONS AMYLOID-BETA ESTROGEN CA2+ DYSREGULATION aged rats neuron intracellular calcium
Indexed ByCSCD
Language英语
CSCD IDCSCD:3427070
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Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/149590
Collection中国科学院金属研究所
Affiliation中国科学院金属研究所
Recommended Citation
GB/T 7714
Li Yu,Cao Yunpeng. Changes in intracellular calcium in brain cells of aged rats[J]. NEURAL REGENERATION RESEARCH,2008,3(9):1026-1029.
APA Li Yu,&Cao Yunpeng.(2008).Changes in intracellular calcium in brain cells of aged rats.NEURAL REGENERATION RESEARCH,3(9),1026-1029.
MLA Li Yu,et al."Changes in intracellular calcium in brain cells of aged rats".NEURAL REGENERATION RESEARCH 3.9(2008):1026-1029.
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