Metabolism Dynamics and Metabolite of SYP-Z048 in Botrytis Cinerea by High Performance Liquid Chromatography-Mass Spectrometry

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	Metabolism Dynamics and Metabolite of SYP-Z048 in Botrytis Cinerea by High Performance Liquid Chromatography-Mass Spectrometry
其他题名	Metabolism Dynamics and Metabolite of SYP-Z048 in Botrytis Cinerea by High Performance Liquid Chromatography-Mass Spectrometry
	Liu PengFei 1; Liu YiXiang 1; Han Ping 3; Li JianQiang 1; Liu JunLi 4; Liu XiLi 1
	2011
发表期刊	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
ISSN	0253-3820
卷号	39 期号:3 页码:317-322
摘要	High performance liquid chromatography-mass spectrometry was used to study the metabolism of a new fungicide 5-(4-chloro phenyl)-2, 3-dimethyl-3-(pyri-dine-3)-oxazoline (SYP-Z048) in Botrytis cinerea. The fungus was cultivated in liquid culture medium with SYP-Z048 and the concentration was determined by high performance liquid chromatography. The linear equation was y = 8466. 1x-46. 3 (r = 0. 9995) in the concentration range of 0. 02 - 0. 31 mg/L. Impact factors of metabolism were studied. The results show that SYP-Z048, at the concentration of 0. 05, 0. 10 and 0. 21 mg/L, was reduced to 46. 2%, 41. 8% and 31. 4% respectively after cultivated with B. cinerea for 44 h, but the degradation rate was less than 0. 6% without fungi at 0. 21 mg/L. The results indicated that SYP-Z048 was metabolized accelerated at lower dosage. The metabolic rate that affected by young mycelium after cultivated for 24 h was obviously higher than that of the mature one. There was a positive correlation between the quantity reduced after a definite period and the amount of B. cinerea put in medium. HPLC-MS was adopted to analyze the metabolic products of SYP-Z048. There were four possible metabolites peaks in chromatogram, of which the compound M-2 is chlorine with a molecule weight of 257. The study showed that SYP-Z048 metabolized in Botrytis cinerea and the metabolize rate was affected by the age and amount of mycelium.
关键词	5-(4-chloro pheny1)-2 3-dimethyl-3-(pyri-dine-3)-oxazoline Botrytis. cinerea Metabolism High performance liquid chromatography-mass spectrometry
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:4170734
引用统计	被引频次：2[CSCD] [CSCD记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/149753
专题	中国科学院金属研究所
作者单位	1.China Agr Univ, Coll Agr & Biotechnology, Beijing 100094, Peoples R China 2.Yunnan Agr University, Coll Plant Protect, Kunming 650201, Peoples R China 3.Beijing Res Ctr Agrifood & Farmland Monitoring, Beijing 100097, Peoples R China 4.中国科学院金属研究所
推荐引用方式 GB/T 7714	Liu PengFei,Liu YiXiang,Han Ping,et al. Metabolism Dynamics and Metabolite of SYP-Z048 in Botrytis Cinerea by High Performance Liquid Chromatography-Mass Spectrometry[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2011,39(3):317-322.
APA	Liu PengFei,Liu YiXiang,Han Ping,Li JianQiang,Liu JunLi,&Liu XiLi.(2011).Metabolism Dynamics and Metabolite of SYP-Z048 in Botrytis Cinerea by High Performance Liquid Chromatography-Mass Spectrometry.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,39(3),317-322.
MLA	Liu PengFei,et al."Metabolism Dynamics and Metabolite of SYP-Z048 in Botrytis Cinerea by High Performance Liquid Chromatography-Mass Spectrometry".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 39.3(2011):317-322.