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Metabolism Dynamics and Metabolite of SYP-Z048 in Botrytis Cinerea by High Performance Liquid Chromatography-Mass Spectrometry
Alternative TitleMetabolism Dynamics and Metabolite of SYP-Z048 in Botrytis Cinerea by High Performance Liquid Chromatography-Mass Spectrometry
Liu PengFei1; Liu YiXiang1; Han Ping3; Li JianQiang1; Liu JunLi4; Liu XiLi1
2011
Source PublicationCHINESE JOURNAL OF ANALYTICAL CHEMISTRY
ISSN0253-3820
Volume39Issue:3Pages:317-322
AbstractHigh performance liquid chromatography-mass spectrometry was used to study the metabolism of a new fungicide 5-(4-chloro phenyl)-2, 3-dimethyl-3-(pyri-dine-3)-oxazoline (SYP-Z048) in Botrytis cinerea. The fungus was cultivated in liquid culture medium with SYP-Z048 and the concentration was determined by high performance liquid chromatography. The linear equation was y = 8466. 1x-46. 3 (r = 0. 9995) in the concentration range of 0. 02 - 0. 31 mg/L. Impact factors of metabolism were studied. The results show that SYP-Z048, at the concentration of 0. 05, 0. 10 and 0. 21 mg/L, was reduced to 46. 2%, 41. 8% and 31. 4% respectively after cultivated with B. cinerea for 44 h, but the degradation rate was less than 0. 6% without fungi at 0. 21 mg/L. The results indicated that SYP-Z048 was metabolized accelerated at lower dosage. The metabolic rate that affected by young mycelium after cultivated for 24 h was obviously higher than that of the mature one. There was a positive correlation between the quantity reduced after a definite period and the amount of B. cinerea put in medium. HPLC-MS was adopted to analyze the metabolic products of SYP-Z048. There were four possible metabolites peaks in chromatogram, of which the compound M-2 is chlorine with a molecule weight of 257. The study showed that SYP-Z048 metabolized in Botrytis cinerea and the metabolize rate was affected by the age and amount of mycelium.
Keyword5-(4-chloro pheny1)-2 3-dimethyl-3-(pyri-dine-3)-oxazoline Botrytis. cinerea Metabolism High performance liquid chromatography-mass spectrometry
Indexed ByCSCD
Language英语
CSCD IDCSCD:4170734
Citation statistics
Cited Times:2[CSCD]   [CSCD Record]
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/149756
Collection中国科学院金属研究所
Affiliation1.China Agr Univ, Coll Agr & Biotechnology, Beijing 100094, Peoples R China
2.Yunnan Agr University, Coll Plant Protect, Kunming 650201, Peoples R China
3.Beijing Res Ctr Agrifood & Farmland Monitoring, Beijing 100097, Peoples R China
4.中国科学院金属研究所
Recommended Citation
GB/T 7714
Liu PengFei,Liu YiXiang,Han Ping,et al. Metabolism Dynamics and Metabolite of SYP-Z048 in Botrytis Cinerea by High Performance Liquid Chromatography-Mass Spectrometry[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2011,39(3):317-322.
APA Liu PengFei,Liu YiXiang,Han Ping,Li JianQiang,Liu JunLi,&Liu XiLi.(2011).Metabolism Dynamics and Metabolite of SYP-Z048 in Botrytis Cinerea by High Performance Liquid Chromatography-Mass Spectrometry.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,39(3),317-322.
MLA Liu PengFei,et al."Metabolism Dynamics and Metabolite of SYP-Z048 in Botrytis Cinerea by High Performance Liquid Chromatography-Mass Spectrometry".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 39.3(2011):317-322.
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