Combined early fluid resuscitation and hydrogen inhalation attenuates lung and intestine injury

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	Combined early fluid resuscitation and hydrogen inhalation attenuates lung and intestine injury
其他题名	Combined early fluid resuscitation and hydrogen inhalation attenuates lung and intestine injury
	Liu Wei; Shan LiPing; Dong XueSong; Liu XiaoWei; Ma Tao; Liu Zhi
	2013
发表期刊	WORLD JOURNAL OF GASTROENTEROLOGY
ISSN	1007-9327
卷号	19 期号:4 页码:492-502
摘要	AIM: To study the effects of combined early fluid resuscitation and hydrogen inhalation on septic shock-induced lung and intestine injuries.
其他摘要	AIM:To study the effects of combined early fluid resuscitation and hydrogen inhalation on septic shockinduced lung and intestine injuries.METHODS:Wistar male rats were randomly divided into four groups:control group(Group A,n = 15);septic shock group(Group B,n = 15);early fluid resuscitation-treated septic shock group(Group C,n = 15);and early fluid resuscitation and inhalation of 2% hydrogentreated septic shock group(Group D,n = 15).The activity of hydroxyl radicals,myeloperoxidase(MPO),superoxide dismutase(SOD),diamine oxidase(DAO),and the concentration of malonaldehyde(MDA) in the lung and intestinal tissue were assessed according to the corresponding kits.Hematoxylin and eosin staining was carried out to detect the pathology of the lung and intestine.The expression levels of interleukin(IL)-6,IL-8,and tumor necrosis factor(TNF)-α in lung and intestine tissue were detected by enzyme-linked immunosorbent assay method.The expression levels of Fas and Bcl2 in lung tissues were determined by immunohistochemistry and Western blotting.RESULTS:Septic shock elicited a significant increase in the levels of MDA(10.17 ± 1.12 nmol/mg protein vs 2.98 ± 0.64 nmol/mg protein) and MPO(6.79 ± 1.02 U/g wet tissue vs 1.69 ± 0.14 U/g wet tissue) in lung tissues.These effects were not significantly decreased by Group C pretreatment,but were significantly reduced by Group D pretreatment(MDA:4.45 ± 1.13 nmol/mg protein vs 9.56 ± 1.37 nmol/mg protein;MPO:2.58 ± 0.21 U/g wet tissue vs 6.02 ± 1.16 U/g wet tissue).The activity of SOD(250.32 ± 8.56 U/mg protein vs 365.78 ± 10.26 U/mg protein) in lung tissues was decreased after septic shock,and was not significantly increased by Group C pretreatment,but was significantly enhanced by Group D pretreatment(331.15 ± 9.64 U/mg protein vs 262.98 ± 5.47 U/mg protein).Histological evidence of lung hemorrhage,neutrophil infiltration and overexpression of IL-6,IL-8,and TNF-α was observed in lung tissues,all of which were attenuated by Group C and further alleviated by Group D pretreatment.Septic shock also elicited a significant increase in the levels of MDA,MPO and DAO(6.54 ± 0.68 kU/L vs 4.32 ± 0.33 kU/L) in intestinal tissues,all of which were further increased by Group C,but significantly reduced by Group D pretreatment.Increased Chiu scoring and overexpression of IL-6,IL-8 and TNF-α were observed in intestinal tissues,all of which were attenuated by Group C and further attenuated by Group D pretreatment.CONCLUSION:Combined early fluid resuscitation and hydrogen inhalation may protect the lung and intestine of the septic shock rats from the damage induced by oxidative stress and the inflammatory reaction.
关键词	OXIDATIVE STRESS POSTPARTUM HEMORRHAGE MOLECULAR-HYDROGEN GENE-EXPRESSION ANTIOXIDANT SEPSIS SHOCK INFLAMMATION LENGTH MODEL Early fluid resuscitation Inhalation of hydrogen gas Septic shock Lung Intestine Oxidative damage
收录类别	CSCD
语种	英语
资助项目	[National Natural Science Foundation of China]
CSCD记录号	CSCD:5008999
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/149911
专题	中国科学院金属研究所
作者单位	中国科学院金属研究所
推荐引用方式 GB/T 7714	Liu Wei,Shan LiPing,Dong XueSong,et al. Combined early fluid resuscitation and hydrogen inhalation attenuates lung and intestine injury[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2013,19(4):492-502.
APA	Liu Wei,Shan LiPing,Dong XueSong,Liu XiaoWei,Ma Tao,&Liu Zhi.(2013).Combined early fluid resuscitation and hydrogen inhalation attenuates lung and intestine injury.WORLD JOURNAL OF GASTROENTEROLOGY,19(4),492-502.
MLA	Liu Wei,et al."Combined early fluid resuscitation and hydrogen inhalation attenuates lung and intestine injury".WORLD JOURNAL OF GASTROENTEROLOGY 19.4(2013):492-502.