Generation and characterization of c305, a murine neutralizing scFv antibody that can inhibit BLyS binding to its receptor BCMA

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	Generation and characterization of c305, a murine neutralizing scFv antibody that can inhibit BLyS binding to its receptor BCMA
其他题名	Generation and Characterization of C305, a Murine Neutralizing scFv Antibody That Can Inhibit BLyS Binding to Its Receptor BCMA
	Liu MY 1; Han W 1; Ding YL 1; Zhou TH 1; Tian RY 1; Yang SL 1; Liu H 1; Gong Y 1
	2005
发表期刊	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
ISSN	1672-9145
卷号	37 期号:6 页码:415-420
摘要	B-lymphocyte stimulator (BLyS) is a member of the tumor necrosis factor (TNF) family and a key regulator of B cell response. Neutralizing single-chain fragment variable (scFv) antibody against BLyS binding to its receptor BCMA has the potential to play a prominent role in autoimmune disease therapy. A phage display scFv library constructed on pIII protein of M13 filamentous phage was screened using BLyS. After five rounds of panning, their binding activity was characterized by phage-ELISA. Nucleotide sequencing revealed that at least two different scFv gene fragments (C305 and D416) were obtained. The two different scFv gene fragments were expressed to obtain the soluble scFv antibodies, then the soluble scFv antibodies were characterized by means of competitive ELISA and in vitro neutralization assay. The results indicated that C305 is the neutralizing scFv antibody that can inhibit BLyS binding to its receptor BCMA.
其他摘要	B-lymphocyte stimulator (BLyS) is a member of the tumor necrosis factor (TNF) family and a key regulator of B cell response. Neutralizing single-chain fragment variable (scFv) antibody against BLyS binding to its receptor BCMA has the potential to play a prominent role in autoimmune disease therapy. A phage display scFv library constructed on pIII protein of M13 filamentous phage was screened using BLyS. After five rounds of panning, their binding activity was characterized by phage-ELISA. Nucleotide sequencing revealed that at least two different scFv gene fragments (C305 and D416) were obtained. The two different scFv gene fragments were expressed to obtain the soluble scFv antibodies, then the soluble scFv antibodies were characterized by means of competitive ELISA and in vitro neutralization assay. The results indicated that C305 is the neutralizing scFv antibody that can inhibit BLyS binding to its receptor BCMA.
关键词	B-LYMPHOCYTE STIMULATOR NECROSIS-FACTOR FAMILY AUTOIMMUNE-DISEASE SJOGRENS-SYNDROME CELL MATURATION TNF FAMILY BAFF MEMBER TACI MICE phage display neutralizing scFv antibody BLyS
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:2147594
引用统计	被引频次：1[CSCD] [CSCD记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/149987
专题	中国科学院金属研究所
作者单位	1.中国科学院 2.暨南大学 3.中国科学院金属研究所
推荐引用方式 GB/T 7714	Liu MY,Han W,Ding YL,et al. Generation and characterization of c305, a murine neutralizing scFv antibody that can inhibit BLyS binding to its receptor BCMA[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2005,37(6):415-420.
APA	Liu MY.,Han W.,Ding YL.,Zhou TH.,Tian RY.,...&Gong Y.(2005).Generation and characterization of c305, a murine neutralizing scFv antibody that can inhibit BLyS binding to its receptor BCMA.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,37(6),415-420.
MLA	Liu MY,et al."Generation and characterization of c305, a murine neutralizing scFv antibody that can inhibit BLyS binding to its receptor BCMA".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 37.6(2005):415-420.