| Biological functions of anti-CEA immunotoxins | |
| 其他题名 | Biological Functions of Anti-CEA Immunotoxins |
| He D1; Yang H1; Chao K1; Lin Q1; Huang HL1 | |
| 2004 | |
| 发表期刊 | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
 |
| ISSN | 1000-3282 |
| 卷号 | 31期号:4页码:368-374 |
| 摘要 | In order to reveal the events during the course of killing target cells of immunotoxins, two forms of Pseudomonas exotoxin A based immunotoxins against carcinoembryonic antigen (CEA) were constructed and named CEA (Fv)/PE38/KDEL and PE35/CEA (Fv)/KDEL. Antigen binding activity of the immunotoxins were assessed by flow cytometry, internalization of immunotoxins was detected by optical confocal microscopy and flow cytometry quantitatively. Cell apoptosis and necrosis were analyzed by staining the cells with FITC-annexin V/PI. The MTT method was used to assay the cytotoxicity of the immunotoxins. The biological functions of the two forms of immunotoxins in the different functional phases were compared. And the interaction between different functional phases was analyzed. The results revealed the whole functional process from the cell binding to the cytotoxicity of the immunotoxins, which provided more details about the biological functions of immunotoxins and could be valuable in the mechanism research and the optimization of immunotoxins. |
| 关键词 | APOPTOSIS CELLS carcinoembryonic antigen (CEA) immunotoxins antigen binding activity endocytosis apoptosis cytotoxicity |
| 收录类别 | CSCD |
| 语种 | 英语 |
| CSCD记录号 | CSCD:1595897 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.imr.ac.cn/handle/321006/150398 |
| 专题 | 中国科学院金属研究所 |
| 作者单位 | 1.中国科学院 2.中国科学院金属研究所 3.NW Sci Tech University Agr & Forestry |
| 推荐引用方式 GB/T 7714 | He D,Yang H,Chao K,et al. Biological functions of anti-CEA immunotoxins[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2004,31(4):368-374. |
| APA | He D,Yang H,Chao K,Lin Q,&Huang HL.(2004).Biological functions of anti-CEA immunotoxins.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,31(4),368-374. |
| MLA | He D,et al."Biological functions of anti-CEA immunotoxins".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 31.4(2004):368-374. |
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