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Biological functions of anti-CEA immunotoxins
Alternative TitleBiological Functions of Anti-CEA Immunotoxins
He D1; Yang H1; Chao K1; Lin Q1; Huang HL1
2004
Source PublicationPROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
ISSN1000-3282
Volume31Issue:4Pages:368-374
AbstractIn order to reveal the events during the course of killing target cells of immunotoxins, two forms of Pseudomonas exotoxin A based immunotoxins against carcinoembryonic antigen (CEA) were constructed and named CEA (Fv)/PE38/KDEL and PE35/CEA (Fv)/KDEL. Antigen binding activity of the immunotoxins were assessed by flow cytometry, internalization of immunotoxins was detected by optical confocal microscopy and flow cytometry quantitatively. Cell apoptosis and necrosis were analyzed by staining the cells with FITC-annexin V/PI. The MTT method was used to assay the cytotoxicity of the immunotoxins. The biological functions of the two forms of immunotoxins in the different functional phases were compared. And the interaction between different functional phases was analyzed. The results revealed the whole functional process from the cell binding to the cytotoxicity of the immunotoxins, which provided more details about the biological functions of immunotoxins and could be valuable in the mechanism research and the optimization of immunotoxins.
KeywordAPOPTOSIS CELLS carcinoembryonic antigen (CEA) immunotoxins antigen binding activity endocytosis apoptosis cytotoxicity
Indexed ByCSCD
Language英语
CSCD IDCSCD:1595897
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Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/150400
Collection中国科学院金属研究所
Affiliation1.中国科学院
2.中国科学院金属研究所
3.NW Sci Tech University Agr & Forestry
Recommended Citation
GB/T 7714
He D,Yang H,Chao K,et al. Biological functions of anti-CEA immunotoxins[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2004,31(4):368-374.
APA He D,Yang H,Chao K,Lin Q,&Huang HL.(2004).Biological functions of anti-CEA immunotoxins.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,31(4),368-374.
MLA He D,et al."Biological functions of anti-CEA immunotoxins".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 31.4(2004):368-374.
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